##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2060994
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29065829400765	32.0	32.0	32.0	32.0	32.0
2	31.42090369986521	32.0	32.0	32.0	32.0	32.0
3	31.494802507916084	32.0	32.0	32.0	32.0	32.0
4	31.596529150497286	32.0	32.0	32.0	32.0	32.0
5	31.53101561673639	32.0	32.0	32.0	32.0	32.0
6	35.12390962807267	36.0	36.0	36.0	36.0	36.0
7	35.13842058734766	36.0	36.0	36.0	36.0	36.0
8	35.08023167461914	36.0	36.0	36.0	36.0	36.0
9	35.202759930402514	36.0	36.0	36.0	36.0	36.0
10	35.08197888979784	36.0	36.0	36.0	36.0	36.0
11	35.21948389951645	36.0	36.0	36.0	36.0	36.0
12	35.12441181294074	36.0	36.0	36.0	36.0	36.0
13	35.177135401655704	36.0	36.0	36.0	36.0	36.0
14	35.138485119316215	36.0	36.0	36.0	36.0	36.0
15	35.090626658786974	36.0	36.0	36.0	36.0	36.0
16	35.10637294431716	36.0	36.0	36.0	36.0	36.0
17	35.077415072532965	36.0	36.0	36.0	36.0	36.0
18	35.09029963211926	36.0	36.0	36.0	36.0	36.0
19	35.07564602322957	36.0	36.0	36.0	36.0	36.0
20	35.07417488842762	36.0	36.0	36.0	36.0	36.0
21	35.07348590049268	36.0	36.0	36.0	36.0	36.0
22	35.05388564935172	36.0	36.0	36.0	36.0	36.0
23	35.021134947505914	36.0	36.0	36.0	36.0	36.0
24	34.99052350467784	36.0	36.0	36.0	36.0	36.0
25	34.975206623600066	36.0	36.0	36.0	36.0	36.0
26	34.928351562401446	36.0	36.0	36.0	32.0	36.0
27	34.914322409478146	36.0	36.0	36.0	32.0	36.0
28	34.90511471649117	36.0	36.0	36.0	32.0	36.0
29	34.878476599155555	36.0	36.0	36.0	32.0	36.0
30	34.86891713415954	36.0	36.0	36.0	32.0	36.0
31	34.86967938771292	36.0	36.0	36.0	32.0	36.0
32	34.83004851057305	36.0	36.0	36.0	32.0	36.0
33	34.821402682395	36.0	36.0	36.0	32.0	36.0
34	34.8145768498113	36.0	36.0	36.0	32.0	36.0
35	34.781043515895725	36.0	36.0	36.0	32.0	36.0
36	34.758242867276664	36.0	36.0	36.0	32.0	36.0
37	34.751428194356706	36.0	36.0	36.0	32.0	36.0
38	34.72676291148834	36.0	36.0	36.0	32.0	36.0
39	34.718664877238844	36.0	36.0	36.0	32.0	36.0
40	34.714453317185786	36.0	36.0	36.0	32.0	36.0
41	34.68132852400347	36.0	36.0	36.0	32.0	36.0
42	34.66747113286113	36.0	36.0	36.0	32.0	36.0
43	34.65953175991779	36.0	36.0	36.0	32.0	36.0
44	34.626499155261975	36.0	36.0	36.0	32.0	36.0
45	34.592145828663256	36.0	36.0	36.0	32.0	36.0
46	34.5944946952781	36.0	36.0	36.0	32.0	36.0
47	34.57346066994858	36.0	36.0	36.0	32.0	36.0
48	34.52962308478336	36.0	36.0	36.0	32.0	36.0
49	34.5235352456145	36.0	36.0	36.0	32.0	36.0
50	34.00146288635484	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	12.0
21	47.0
22	155.0
23	453.0
24	1222.0
25	2994.0
26	6047.0
27	11016.0
28	19393.0
29	29863.0
30	43467.0
31	63277.0
32	94642.0
33	158360.0
34	359813.0
35	1270230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.709400498282605	18.147716431194006	11.357912298767687	26.784970771755706
2	16.835847923194944	20.553193595438124	35.78202984512637	26.82892863624057
3	18.456523679950937	23.7831055457005	28.763056869922327	28.997313904426235
4	12.736943283054883	15.778201630382307	35.964820772939596	35.52003431362321
5	14.668067932269574	36.38326943212838	33.41295510806921	15.53570752753283
6	34.4007052951968	35.45923033739758	16.54013307255137	13.599931294854247
7	30.295042101044444	30.50401893455294	20.734412618377345	18.466526346025265
8	28.319247896888587	32.393495565731875	19.49758223459166	19.789674302787876
9	27.75345294552046	14.004844264466563	18.543479505520153	39.698223284492826
10	16.150604999335272	26.267422418502917	31.49203733732364	26.08993524483817
11	37.68914417024018	21.028736619320583	22.096522357658486	19.185596852780744
12	24.65247351520674	23.851694861799693	28.296783008587116	23.199048614406447
13	29.48310378390233	19.235815339588566	25.296822795214347	25.984258081294755
14	23.585658182411013	19.591566011351805	24.71360906436409	32.109166741873096
15	25.582704267940613	26.73428452484578	21.956444317644788	25.72656688956882
16	26.238892495562823	25.540588667409995	23.28182420715441	24.93869462987277
17	24.52792196386792	25.436900835228048	24.906331605041064	25.12884559586297
18	25.268098791165816	24.57323990268773	25.827003863184462	24.331657442961987
19	25.95912457775229	24.763196787569495	25.050630909163246	24.227047725514968
20	25.854514860305272	24.271152657407058	24.700654150375982	25.17367833191169
21	26.955439947908634	23.996430848415862	24.090172023790462	24.957957179885046
22	26.048595968741296	24.352181520179098	24.491628796590383	25.107593714489223
23	24.891727001631253	24.012248458753398	25.154076139959653	25.9419483996557
24	24.962954768427274	24.96906832334301	24.754802779629635	25.313174128600085
25	25.060334964585053	24.36178853504668	25.09842338211562	25.47945311825265
26	24.744079798388544	24.9221492153786	25.475474455529714	24.858296530703146
27	25.630448220615875	24.660624921761055	24.509290177458062	25.199636680165007
28	24.770523349412954	24.50603931889176	25.47945311825265	25.243984213442637
29	24.911765876077272	24.733453857701672	25.027438216705143	25.32734204951591
30	24.908223895848312	24.79895623179883	25.388234997287718	24.90458487506514
31	25.290563679467287	24.523846260590766	24.532434349639058	25.653155710302894
32	25.014046620223056	24.66237165173698	24.660527881206836	25.66305384683313
33	24.922391816764144	24.166639980514258	25.100703835139743	25.81026436758186
34	25.208079208381974	24.40647571026408	25.426371935095393	24.959073146258554
35	25.92050243717352	24.314141622925636	24.92156697205329	24.84378896784755
36	24.893764853269833	24.638790797062	24.911135112474856	25.556309237193314
37	25.859560969124608	24.449270594674218	24.48114841673484	25.210020019466334
38	24.84291560285959	24.402060365047156	25.24209192263539	25.512932109457864
39	25.18629360396003	24.446504938879006	24.53971239120541	25.827489065955554
40	25.49449440415644	24.452812574903177	25.282848955406955	24.769844065533427
41	24.6242347139293	24.707495509448353	25.580181213530945	25.088088563091404
42	25.5912438367118	24.886292730595045	25.06766152642851	24.45480190626465
43	24.812638949943572	23.97095770293363	25.415260791637433	25.801142555485363
44	24.62253650423048	24.351017033528482	25.205750235080743	25.820696227160294
45	24.697112170147026	24.730542641075132	25.274503467744204	25.297841721033638
46	24.644552062575723	24.292391378710533	25.278470405741704	25.78458615297204
47	24.77493869462987	24.098954193947193	25.628750010917063	25.497357100505873
48	25.179791886827424	24.90642864559528	24.952765510234382	24.96101395734291
49	24.333792335154786	24.72583617419556	25.394154471095014	25.54621701955464
50	24.06984202768179	25.085080305910644	25.286342415358803	25.558735251048763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	18.0
2	29.0
3	85.5
4	142.0
5	167.5
6	193.0
7	201.0
8	209.0
9	302.0
10	395.0
11	564.5
12	734.0
13	1316.0
14	1898.0
15	2583.0
16	3268.0
17	4169.0
18	5070.0
19	5537.0
20	6004.0
21	7333.5
22	8663.0
23	9260.5
24	9858.0
25	12579.5
26	15301.0
27	19512.5
28	23724.0
29	26724.5
30	29725.0
31	31092.5
32	32460.0
33	37355.5
34	42251.0
35	49191.0
36	56131.0
37	68275.0
38	80419.0
39	85505.0
40	90591.0
41	99454.0
42	108317.0
43	108474.5
44	108632.0
45	118299.5
46	127967.0
47	135074.0
48	142181.0
49	145905.5
50	149630.0
51	145016.5
52	140403.0
53	138163.0
54	135923.0
55	137420.5
56	138918.0
57	137659.0
58	136400.0
59	125288.0
60	114176.0
61	101136.0
62	88096.0
63	78773.5
64	69451.0
65	59817.0
66	50183.0
67	46027.5
68	41872.0
69	38423.5
70	34975.0
71	28369.5
72	21764.0
73	19280.5
74	16797.0
75	12900.0
76	9003.0
77	7873.0
78	6743.0
79	5874.5
80	5006.0
81	3913.0
82	2820.0
83	2496.5
84	2173.0
85	1775.5
86	1378.0
87	1051.0
88	724.0
89	499.5
90	275.0
91	171.5
92	68.0
93	50.5
94	33.0
95	21.0
96	9.0
97	6.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015332407566446092
2	0.0042697843855925835
3	4.852027710900662E-4
4	2.426013855450331E-4
5	0.0
6	6.307636024170862E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.4556083132701987E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2060994.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.71429575664122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.35185561918298	44.51109488699553
2	11.639285127624353	12.736705777374285
3	2.962659357288987	4.862994609026707
4	1.2051963651625939	2.6376588147334052
5	0.6491295801607422	1.7758333916649593
6	0.4274602356161155	1.4032911453422212
7	0.2986555646894614	1.143851022705012
8	0.21107331492389658	0.9238982223264599
9	0.16239834957580876	0.7996960196173089
>10	0.8997758538547442	9.062122501263607
>50	0.09057892860288144	3.52930851148296
>100	0.0876774524951796	10.207597440398105
>500	0.011225222522410231	4.120661328559974
>1k	0.0030290282996979986	2.2852863285094784
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	4.852027710900662E-5	0.0
7	0.0	0.0	0.0	9.704055421801324E-5	0.0
8	0.0	0.0	0.0	9.704055421801324E-5	0.0
9	0.0	0.0	0.0	2.4260138554503313E-4	9.704055421801324E-5
10	0.0	0.0	0.0	2.4260138554503313E-4	1.4556083132701987E-4
11	0.0	0.0	0.0	2.4260138554503313E-4	1.4556083132701987E-4
12	0.0	0.0	0.0	2.4260138554503313E-4	1.940811084360265E-4
13	0.0	0.0	0.0	2.4260138554503313E-4	2.9112166265403975E-4
14	0.0	0.0	0.0	2.4260138554503313E-4	3.3964193976304636E-4
15	0.0	0.0	0.0	2.9112166265403975E-4	4.8520277109006626E-4
16	0.0	0.0	0.0	3.3964193976304636E-4	5.337230481990728E-4
17	0.0	0.0	0.0	3.3964193976304636E-4	5.337230481990728E-4
18	0.0	0.0	0.0	4.8520277109006626E-4	5.337230481990728E-4
19	0.0	0.0	0.0	5.337230481990728E-4	5.337230481990728E-4
20	0.0	0.0	0.0	6.792838795260927E-4	6.307636024170862E-4
21	0.0	0.0	0.0	9.218852650711259E-4	6.792838795260927E-4
22	0.0	0.0	0.0	9.704055421801325E-4	7.278041566350994E-4
23	0.0	0.0	0.0	0.0012615272048341723	7.76324433744106E-4
24	0.0	0.0	0.0	0.0017952502530332452	7.76324433744106E-4
25	0.0	0.0	0.0	0.002231932747014305	7.76324433744106E-4
26	0.0	0.0	0.0	0.0029112166265403976	8.248447108531126E-4
27	0.0	0.0	0.0	0.004997588542227683	8.248447108531126E-4
28	0.0	0.0	0.0	0.011741907060379603	8.248447108531126E-4
29	0.0	0.0	0.0	0.026831713241280662	8.248447108531126E-4
30	0.0	0.0	0.0	0.05482791313317749	8.248447108531126E-4
31	0.0	0.0	0.0	0.11766167198934106	8.248447108531126E-4
32	0.0	0.0	0.0	0.19432370982157154	8.733649879621193E-4
33	0.0	0.0	0.0	0.2732662006779253	8.733649879621193E-4
34	0.0	0.0	0.0	0.366134011064564	0.0010674460963981456
35	0.0	0.0	0.0	0.46040890948736385	0.0010674460963981456
36	0.0	0.0	0.0	0.6032526052962793	0.0010674460963981456
37	0.0	0.0	0.0	0.8060188433348181	0.0011159663735071524
38	0.0	0.0	0.0	1.0734140904825535	0.001164486650616159
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	165	0.0	21.333275	2
GTATCAA	3100	0.0	21.22187	1
CGCAATA	610	0.0	19.475357	36
ATACCGT	465	0.0	18.924679	6
TACCGTC	460	0.0	18.65212	7
TTAACGG	360	0.0	18.33328	35
CGTCGTA	460	0.0	17.695602	10
CTAGCGG	710	0.0	17.352066	29
ACCGTCG	475	0.0	17.136795	8
CAATACG	720	0.0	17.111063	38
GTATTAG	610	0.0	16.95283	1
TATTGGG	185	4.2382453E-10	16.648603	2
ATACGAA	715	0.0	16.61534	40
TAACGGC	400	0.0	16.499954	36
TCTAGCG	735	0.0	16.46254	28
TGCGTAG	215	1.2732926E-11	16.37205	13
TAGCGGC	735	0.0	16.163221	30
AATACGA	740	0.0	16.05401	39
GTATATA	275	0.0	16.001898	1
GCAATAC	770	0.0	15.999955	37
>>END_MODULE