##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062554_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1000827
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.097338501059625	32.0	32.0	32.0	32.0	32.0
2	30.778368289424645	32.0	32.0	32.0	32.0	32.0
3	30.776437885868386	32.0	32.0	32.0	32.0	32.0
4	30.84355837722204	32.0	32.0	32.0	32.0	32.0
5	30.628542195604236	32.0	32.0	32.0	32.0	32.0
6	34.26957106472947	36.0	36.0	36.0	32.0	36.0
7	34.13116352776254	36.0	36.0	36.0	32.0	36.0
8	34.11185549550522	36.0	36.0	36.0	32.0	36.0
9	34.2765782697709	36.0	36.0	36.0	32.0	36.0
10	33.95336956337109	36.0	36.0	36.0	32.0	36.0
11	34.296884476537905	36.0	36.0	36.0	32.0	36.0
12	34.070064057024844	36.0	36.0	36.0	32.0	36.0
13	34.19398057806194	36.0	36.0	36.0	32.0	36.0
14	34.092916158337054	36.0	36.0	36.0	32.0	36.0
15	34.033415365492736	36.0	36.0	36.0	32.0	36.0
16	34.03702238248968	36.0	36.0	36.0	32.0	36.0
17	33.953893130381175	36.0	36.0	36.0	32.0	36.0
18	33.98678792638488	36.0	36.0	36.0	32.0	36.0
19	33.9633932737626	36.0	36.0	36.0	32.0	36.0
20	33.9481498800492	36.0	36.0	36.0	32.0	36.0
21	33.91309786806311	36.0	36.0	36.0	32.0	36.0
22	33.87354957450189	36.0	36.0	36.0	32.0	36.0
23	33.83837366497906	36.0	36.0	36.0	32.0	36.0
24	33.82166048677743	36.0	36.0	36.0	32.0	36.0
25	33.34688112930606	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	42.0
5	110.0
6	189.0
7	53.0
8	136.0
9	170.0
10	136.0
11	47.0
12	77.0
13	63.0
14	256.0
15	286.0
16	434.0
17	572.0
18	709.0
19	1077.0
20	1529.0
21	2285.0
22	3306.0
23	4719.0
24	6592.0
25	9114.0
26	12311.0
27	16057.0
28	21338.0
29	28373.0
30	36594.0
31	49561.0
32	71624.0
33	103299.0
34	224881.0
35	404884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.5903661171279	17.66931343295514	11.028481550246923	25.711838899670035
2	16.553005208942235	20.626631853785902	37.004473515131714	25.815889422140152
3	19.195688971224502	24.749046245835757	28.362320665239782	27.69294411769996
4	12.560360403575254	15.43157688556307	36.38023456612462	35.62782814473705
5	14.073463969709207	37.044948402496516	33.95739457596488	14.924193051829402
6	33.291481273035714	35.70653841809209	17.415921843276696	13.586058465595507
7	29.413917399499056	31.005534363548193	21.246656634487035	18.333891602465716
8	28.082139721133785	32.498754882959	20.204399213526074	19.214706182381143
9	27.457787486172542	14.345453490721349	18.93977127928771	39.2569877438184
10	15.503920173914262	27.008632686156993	32.28128410831425	25.206163031614498
11	36.9363565060106	21.703175771933473	22.276423414915243	19.084044307140687
12	24.024746674778143	24.394165740283988	29.351405383392354	22.22968220154552
13	29.245101805608915	19.901415834293765	25.686007331284415	25.16747502881291
14	23.225187441096374	19.952897107994485	25.764821703347828	31.057093747561314
15	25.001300478576116	27.080865758599167	23.374801927109175	24.543031835715542
16	25.292211040195273	26.186650927352396	24.43138392589184	24.089754106560495
17	23.35649433480903	25.956229208874216	26.16582876866512	24.521447687651634
18	24.168492670969034	25.21406773725549	27.249086997848813	23.36835259392666
19	24.793708445271577	25.960667777294173	26.040312776718842	23.205311000715405
20	24.71527016319442	24.78640259958541	26.46136638307068	24.03696085414949
21	25.850165890908215	24.83621024321328	24.948972036676096	24.3646518292024
22	24.88361247658239	25.1861399772629	25.65169007093334	24.278557475221366
23	23.716651757393098	24.994046296796608	26.010578579876682	25.278723365933608
24	23.6958620793164	26.109874276451723	25.83431307314779	24.359950571084084
25	24.227964572018617	25.250241136000707	25.891903033310786	24.62989125866989
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	217.0
1	217.0
2	531.0
3	845.0
4	845.0
5	845.0
6	2305.0
7	3765.0
8	3765.0
9	3765.0
10	3831.0
11	3897.0
12	3897.0
13	3897.0
14	3916.0
15	3935.0
16	3935.0
17	3935.0
18	6466.0
19	8997.0
20	8997.0
21	8997.0
22	13808.5
23	18620.0
24	18620.0
25	18620.0
26	26732.5
27	34845.0
28	34845.0
29	34845.0
30	42678.0
31	50511.0
32	50511.0
33	50511.0
34	59324.5
35	68138.0
36	68138.0
37	68138.0
38	75814.0
39	83490.0
40	83490.0
41	83490.0
42	93080.5
43	102671.0
44	102671.0
45	102671.0
46	119216.5
47	135762.0
48	135762.0
49	135762.0
50	132082.5
51	128403.0
52	128403.0
53	128403.0
54	116447.0
55	104491.0
56	104491.0
57	104491.0
58	94506.5
59	84522.0
60	84522.0
61	84522.0
62	75839.0
63	67156.0
64	67156.0
65	67156.0
66	56737.0
67	46318.0
68	46318.0
69	46318.0
70	35470.5
71	24623.0
72	24623.0
73	24623.0
74	19517.0
75	14411.0
76	14411.0
77	14411.0
78	11468.0
79	8525.0
80	8525.0
81	8525.0
82	6156.5
83	3788.0
84	3788.0
85	3788.0
86	2777.5
87	1767.0
88	1767.0
89	1767.0
90	1141.5
91	516.0
92	516.0
93	516.0
94	324.0
95	132.0
96	132.0
97	132.0
98	307.0
99	482.0
100	482.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010890993148666053
2	0.004796033680146519
3	0.004496281575137362
4	0.017085869985521973
5	0.039067691019526854
6	0.04776050206479242
7	0.07114116625550669
8	0.09202389623781132
9	0.10041695517806774
10	0.1252963798938278
11	0.12579596673550972
12	0.14188266303766786
13	0.1273946446288919
14	0.13119150462567458
15	0.11940125516198104
16	0.12060026358201767
17	0.13009241357397433
18	0.13758621619920328
19	0.13908497672424905
20	0.12819398357558298
21	0.1373863814625305
22	0.1577695246031532
23	0.14478026671942304
24	0.14108332409097676
25	0.13898505935591265
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1000827.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.75710554065508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92386939038991	43.763997514271125
2	12.698056999391422	13.9061769455386
3	3.429672546122454	5.633968247337433
4	1.3018621995436166	2.851448234391967
5	0.6653193496059777	1.8215480922307257
6	0.37611068751464233	1.235683956672457
7	0.2420516424151795	0.9277833131011819
8	0.18196687302518372	0.7971183416914372
9	0.13653480845265953	0.6728625824773863
>10	0.8220444267243483	8.92261694100111
>50	0.11299744982167569	4.431224166310005
>100	0.10146922047030672	10.824902842771332
>500	0.006216132312900599	2.2878367711330947
>1k	0.0018282742096766469	1.9228320510722163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2960	0.29575541027570196	No Hit
GTACATGGAAGCAGTGGTATCAACG	2952	0.2949560713290109	No Hit
TCCATGTACTCTGCGTTGATACCAC	2454	0.24519722189749077	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2297	0.22951019506867823	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1943	0.19413944667759764	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1712	0.1710585345918925	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1371	0.13698671198918494	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1330	0.13289009988739314	No Hit
CATGTACTCTGCGTTGATACCACTG	1177	0.1176027425319261	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1011	0.10101645938808605	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.998347366727716E-4	0.0
8	0.0	0.0	0.0	1.998347366727716E-4	0.0
9	0.0	0.0	0.0	1.998347366727716E-4	0.0
10	0.0	0.0	0.0	1.998347366727716E-4	0.0
11	0.0	0.0	0.0	1.998347366727716E-4	0.0
12	0.0	0.0	0.0	1.998347366727716E-4	0.0
13	0.0	0.0	0.0	3.996694733455432E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCTAA	45	6.7515095E-4	14.780036	9
GCGTTCC	65	3.3638717E-6	14.617618	9
ACTGCGC	40	0.005272007	14.252178	8
CAAGACG	165	0.0	13.816144	4
CGACATG	50	0.0014977334	13.301366	15
GTACGCC	65	5.456234E-5	13.151905	4
CGGCGTC	95	7.3663614E-8	13.001336	14
GGTTTAC	60	4.0844828E-4	12.668603	8
AAGACGG	180	0.0	12.665432	5
TAGATTA	105	1.9928848E-8	12.665432	5
TTAAGGC	60	4.101767E-4	12.662264	3
GCGCAAG	225	0.0	12.657196	1
GCATATA	60	4.1156405E-4	12.657196	1
ACCCAAT	100	1.438475E-7	12.351268	12
CGCAAGA	200	0.0	12.343235	2
GTATTAT	100	1.4545003E-7	12.340766	1
TAGGACA	85	3.9458337E-6	12.292304	4
TAGTACG	70	1.0957387E-4	12.207596	2
CGTCGTA	125	1.4042598E-9	12.161858	10
CGAACGA	55	0.0030636173	12.092756	16
>>END_MODULE