FastQCFastQC Report
Thu 2 Feb 2017
SRR4062554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000827
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA12030.12020059410867212No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11700.1169033209535714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA300.00574492929.33293528
ATGCGTA602.8725635E-421.999722
CATTACG550.004483435319.9997312
CTATACT1452.9467628E-1019.7248544
CGAGCCG2700.019.5552915
TATGGCG2200.018.99974316
GTATTAG2800.018.8625411
GATATAC1901.8189894E-1218.5316181
GGCGCAT600.007411814818.33308415
GTGTATA2750.017.6050381
TGGCGAG2500.017.5997618
AAGGCGT1408.364259E-817.2863436
CATTCCG1154.2719566E-617.2171579
ATTGTCG1451.2487726E-716.68942843
GAGCGAA2900.016.68942816
TGCGTAT800.00198820916.49977523
CGTCGTA1753.623427E-916.34263410
CTAGGTG1753.623427E-916.34263427
ATAACGC2301.8189894E-1216.261463
ACGATTA953.3459405E-416.21030640
Produced by FastQC (version 0.11.5)