##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062554_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1000827
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.164830685023485	32.0	32.0	32.0	32.0	32.0
2	31.339462264707088	32.0	32.0	32.0	32.0	32.0
3	31.452108106595844	32.0	32.0	32.0	32.0	32.0
4	31.5745498472763	32.0	32.0	32.0	32.0	32.0
5	31.480724440887386	32.0	32.0	32.0	32.0	32.0
6	35.08159152380981	36.0	36.0	36.0	36.0	36.0
7	35.10380914983309	36.0	36.0	36.0	36.0	36.0
8	35.038672018240916	36.0	36.0	36.0	36.0	36.0
9	35.16325798564587	36.0	36.0	36.0	36.0	36.0
10	35.03925253815095	36.0	36.0	36.0	36.0	36.0
11	35.17960246875834	36.0	36.0	36.0	36.0	36.0
12	35.08377571748164	36.0	36.0	36.0	36.0	36.0
13	35.13253339488243	36.0	36.0	36.0	36.0	36.0
14	35.08368079598172	36.0	36.0	36.0	36.0	36.0
15	35.04163057151735	36.0	36.0	36.0	36.0	36.0
16	35.06649201110682	36.0	36.0	36.0	36.0	36.0
17	35.037731795804866	36.0	36.0	36.0	36.0	36.0
18	35.052221812561015	36.0	36.0	36.0	36.0	36.0
19	35.02795488131316	36.0	36.0	36.0	36.0	36.0
20	35.01409134645648	36.0	36.0	36.0	36.0	36.0
21	35.00462217745924	36.0	36.0	36.0	36.0	36.0
22	34.98583571386463	36.0	36.0	36.0	36.0	36.0
23	34.94786811307049	36.0	36.0	36.0	32.0	36.0
24	34.92227327999744	36.0	36.0	36.0	32.0	36.0
25	34.904553933896665	36.0	36.0	36.0	32.0	36.0
26	34.86048637776559	36.0	36.0	36.0	32.0	36.0
27	34.83606257624944	36.0	36.0	36.0	32.0	36.0
28	34.82059836515202	36.0	36.0	36.0	32.0	36.0
29	34.78831906013727	36.0	36.0	36.0	32.0	36.0
30	34.77031494953673	36.0	36.0	36.0	32.0	36.0
31	34.774953113774906	36.0	36.0	36.0	32.0	36.0
32	34.72078091418397	36.0	36.0	36.0	32.0	36.0
33	34.70340928052501	36.0	36.0	36.0	32.0	36.0
34	34.679854760113386	36.0	36.0	36.0	32.0	36.0
35	34.63135387034922	36.0	36.0	36.0	32.0	36.0
36	34.597177134509764	36.0	36.0	36.0	32.0	36.0
37	34.58214856313828	36.0	36.0	36.0	32.0	36.0
38	34.53639739935074	36.0	36.0	36.0	32.0	36.0
39	34.507542262548874	36.0	36.0	36.0	32.0	36.0
40	34.47609127251763	36.0	36.0	36.0	32.0	36.0
41	34.452357900016686	36.0	36.0	36.0	32.0	36.0
42	34.39372538910321	36.0	36.0	36.0	32.0	36.0
43	34.4003868800502	36.0	36.0	36.0	32.0	36.0
44	34.349816701587784	36.0	36.0	36.0	32.0	36.0
45	34.27515144975106	36.0	36.0	36.0	32.0	36.0
46	34.28815669441372	36.0	36.0	36.0	32.0	36.0
47	34.22702924681288	36.0	36.0	36.0	32.0	36.0
48	34.18533372900611	36.0	36.0	36.0	32.0	36.0
49	34.17497729377804	36.0	36.0	36.0	32.0	36.0
50	33.63197635555396	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	8.0
21	26.0
22	113.0
23	261.0
24	778.0
25	1802.0
26	3778.0
27	7003.0
28	11564.0
29	17500.0
30	24771.0
31	35126.0
32	51819.0
33	82404.0
34	177143.0
35	586727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.009405397411015	18.357712885554342	11.496029787089464	26.136851929945177
2	16.045984132376745	21.11313175722936	36.71286396610643	26.128020144287454
3	18.608715288324724	24.922563188438236	28.757334015443398	27.71138750779364
4	12.231109334798791	15.869807408887668	36.60060450128644	35.298478755027105
5	13.817586673407764	37.291397305825384	33.724443609578486	15.166572411188358
6	33.399612317899326	36.091804720129495	16.965188545392778	13.543394416578405
7	29.380631598299807	31.079927979488943	21.19649169785757	18.342948724353683
8	28.06828752621582	32.703753995445766	20.06890301720477	19.159055461133644
9	27.611165566076856	14.076658603335042	18.785564338292232	39.52661149229587
10	15.48339523214302	27.05452590707485	32.28579964369466	25.176279217087465
11	37.03697042545815	21.589345611179557	22.36280595947152	19.01087800389078
12	24.050909897514757	24.295607532570564	29.325947441465907	22.327535128448773
13	29.480519610282297	19.815512571103696	25.654883411418755	25.04908440719525
14	23.27954781395786	19.88955134104096	25.636398698276526	31.194502146724655
15	25.078460113486145	27.218989895356536	23.095400104113896	24.607149887043413
16	25.360327009563093	26.151772484155604	24.436690856661542	24.051209649619764
17	23.494370155881086	25.843727237574527	26.1330879362767	24.52881467026769
18	24.291211168363763	25.071565815070933	27.331796604208318	23.30542641235698
19	24.915195133624493	25.836633104422642	25.985709817980528	23.262461943972333
20	24.936177780975132	24.60285344020495	26.48359806440074	23.977370714419173
21	26.032770898466968	24.742537921139217	25.05507944929543	24.16961173109838
22	24.961856544637584	25.065770607707428	25.626007291969543	24.34636555568545
23	23.782232094058216	24.75442808797125	26.137384383115165	25.325955434855373
24	23.951492116020052	25.819147564963774	25.896183855951126	24.333176463065044
25	24.516824586067322	24.934579103081752	25.995301885340822	24.553294425510103
26	23.60987463367795	25.496214630500575	26.65645511162269	24.237455624198788
27	24.778907843213663	25.502909094179117	25.81305260549526	23.905130457111966
28	23.914023102893907	25.06916779823086	26.52006790384352	24.49674119503171
29	23.66842621152307	25.641394566693343	26.530159558045497	24.16001966373809
30	24.250644716819192	25.47653090893831	26.445129877591235	23.82769449665127
31	23.915521863418952	25.447754706857427	25.87839856438725	24.758324865336366
32	23.898535910801765	25.57984546779813	25.916167329618407	24.605451291781698
33	23.78732787984337	24.950266129910563	26.289658452459815	24.97274753778625
34	23.938902527609667	25.658180684573857	26.63787048111212	23.765046306704356
35	25.009916798807385	25.224339471257267	26.076234953693294	23.68950877624205
36	23.862365823463996	25.827640541272366	26.013886515851393	24.296107119412248
37	24.801988755299366	25.56086116781422	25.67446721561269	23.962682861273727
38	23.91941864078407	25.42667214213845	26.002396018492707	24.65151319858477
39	24.162117928473155	25.364023952191538	25.794767727089695	24.679090392245612
40	24.708865768009854	25.45414941843096	26.107209337877574	23.72977547568161
41	23.41083923595187	25.706740525585342	26.666746600561336	24.215673637901457
42	24.89840901574398	25.900080633316247	25.89478501279442	23.306725338145355
43	23.956587901805207	24.87023231787312	26.241198528816668	24.931981251505007
44	23.647843233645773	25.404690321104447	26.284462749306325	24.663003695943456
45	23.93500575024455	25.66587432193576	26.09212181525878	24.306998112560912
46	23.62616116471678	25.19456409549303	26.184745215706613	24.994529524083582
47	23.817103255607613	25.140508799223042	26.578019977478622	24.46436796769072
48	24.291411003100436	26.119599091551287	25.44485710317567	24.144132802172603
49	23.294735253944985	25.69025415980984	26.534855674357306	24.48015491188787
50	23.200812927708785	26.537353608565716	25.606923074617292	24.65491038910821
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	40.5
2	51.0
3	153.5
4	256.0
5	288.0
6	320.0
7	271.5
8	223.0
9	314.0
10	405.0
11	599.5
12	794.0
13	1410.5
14	2027.0
15	2711.0
16	3395.0
17	4004.5
18	4614.0
19	5039.0
20	5464.0
21	6186.5
22	6909.0
23	7324.0
24	7739.0
25	8948.5
26	10158.0
27	11975.0
28	13792.0
29	15741.0
30	17690.0
31	18733.5
32	19777.0
33	22580.5
34	25384.0
35	28636.0
36	31888.0
37	37242.0
38	42596.0
39	45221.0
40	47846.0
41	51635.5
42	55425.0
43	56146.0
44	56867.0
45	61754.0
46	66641.0
47	69809.5
48	72978.0
49	73133.5
50	73289.0
51	68621.0
52	63953.0
53	61634.0
54	59315.0
55	60126.0
56	60937.0
57	59181.0
58	57425.0
59	52604.5
60	47784.0
61	42204.5
62	36625.0
63	32708.5
64	28792.0
65	24890.0
66	20988.0
67	19315.0
68	17642.0
69	16397.5
70	15153.0
71	11931.0
72	8709.0
73	7641.0
74	6573.0
75	4911.0
76	3249.0
77	2919.0
78	2589.0
79	2242.5
80	1896.0
81	1477.5
82	1059.0
83	904.0
84	749.0
85	626.0
86	503.0
87	357.0
88	211.0
89	143.0
90	75.0
91	49.5
92	24.0
93	16.0
94	8.0
95	6.0
96	4.0
97	3.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012489671042048225
2	0.004696116311810133
3	0.0010990910517002439
4	1.998347366727716E-4
5	9.99173683363858E-5
6	6.994215783547006E-4
7	9.99173683363858E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1000827.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.07068824027858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.85547151724718	47.76188350516033
2	12.295182639164057	14.525698010706911
3	3.2166519980220887	5.700295420578961
4	1.2750377234042458	3.01269423415227
5	0.612753071878339	1.8097872838599185
6	0.3363644725437946	1.192156853564414
7	0.23602841121221724	0.975965248619561
8	0.1539129621550673	0.7273395682879822
9	0.13040127427443526	0.6932603716920201
>10	0.6996602887616978	8.105071881186054
>50	0.10201107703201523	4.222283238710319
>100	0.08108490630922335	9.043975108207084
>500	0.005099679370998412	1.9913071932067854
>1k	3.399786247332275E-4	0.23828208206745508
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1203	0.12020059410867212	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1170	0.1169033209535714	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.99173683363858E-5
7	0.0	0.0	0.0	9.99173683363858E-5	9.99173683363858E-5
8	0.0	0.0	0.0	9.99173683363858E-5	9.99173683363858E-5
9	0.0	0.0	0.0	9.99173683363858E-5	9.99173683363858E-5
10	0.0	0.0	0.0	9.99173683363858E-5	9.99173683363858E-5
11	0.0	0.0	0.0	2.9975210500915743E-4	9.99173683363858E-5
12	0.0	0.0	0.0	2.9975210500915743E-4	9.99173683363858E-5
13	0.0	0.0	0.0	3.996694733455432E-4	9.99173683363858E-5
14	0.0	0.0	0.0	3.996694733455432E-4	9.99173683363858E-5
15	0.0	0.0	0.0	4.99586841681929E-4	9.99173683363858E-5
16	0.0	0.0	0.0	4.99586841681929E-4	9.99173683363858E-5
17	0.0	0.0	0.0	4.99586841681929E-4	9.99173683363858E-5
18	0.0	0.0	0.0	5.995042100183149E-4	9.99173683363858E-5
19	0.0	0.0	0.0	8.992563150274723E-4	9.99173683363858E-5
20	0.0	0.0	0.0	0.0010990910517002439	9.99173683363858E-5
21	0.0	0.0	0.0	0.0015986778933821729	9.99173683363858E-5
22	0.0	0.0	0.0	0.003696942628446275	9.99173683363858E-5
23	0.0	0.0	0.0	0.005495455258501219	9.99173683363858E-5
24	0.0	0.0	0.0	0.008692811045265566	9.99173683363858E-5
25	0.0	0.0	0.0	0.011190745253675211	9.99173683363858E-5
26	0.0	0.0	0.0	0.01358876209374847	9.99173683363858E-5
27	0.0	0.0	0.0	0.01788520893221306	9.99173683363858E-5
28	0.0	0.0	0.0	0.028376532607533568	9.99173683363858E-5
29	0.0	0.0	0.0	0.044463228909691684	9.99173683363858E-5
30	0.0	0.0	0.0	0.07773571256570816	9.99173683363858E-5
31	0.0	0.0	0.0	0.1322905956773748	9.99173683363858E-5
32	0.0	0.0	0.0	0.20602961350962753	9.99173683363858E-5
33	0.0	0.0	0.0	0.27687102766012506	9.99173683363858E-5
34	0.0	0.0	0.0	0.3617008733777166	9.99173683363858E-5
35	0.0	0.0	0.0	0.4569221254022923	9.99173683363858E-5
36	0.0	0.0	0.0	0.5892127210796672	9.99173683363858E-5
37	0.0	0.0	0.0	0.7869491930173746	9.99173683363858E-5
38	0.0	0.0	0.0	1.0353437707016298	1.998347366727716E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGCA	30	0.005744929	29.332935	28
ATGCGTA	60	2.8725635E-4	21.9997	22
CATTACG	55	0.0044834353	19.99973	12
CTATACT	145	2.9467628E-10	19.724854	4
CGAGCCG	270	0.0	19.55529	15
TATGGCG	220	0.0	18.999743	16
GTATTAG	280	0.0	18.862541	1
GATATAC	190	1.8189894E-12	18.531618	1
GGCGCAT	60	0.0074118148	18.333084	15
GTGTATA	275	0.0	17.605038	1
TGGCGAG	250	0.0	17.59976	18
AAGGCGT	140	8.364259E-8	17.286343	6
CATTCCG	115	4.2719566E-6	17.217157	9
ATTGTCG	145	1.2487726E-7	16.689428	43
GAGCGAA	290	0.0	16.689428	16
TGCGTAT	80	0.001988209	16.499775	23
CGTCGTA	175	3.623427E-9	16.342634	10
CTAGGTG	175	3.623427E-9	16.342634	27
ATAACGC	230	1.8189894E-12	16.26146	3
ACGATTA	95	3.3459405E-4	16.210306	40
>>END_MODULE