FastQCFastQC Report
Thu 2 Feb 2017
SRR4062553_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062553_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130709
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG60280.28291052414947326No Hit
TCCATGTACTCTGCGTTGATACCAC52000.24405021990332795No Hit
GTATCAACGCAGAGTACTTTTTTTT36440.17102288487071676No Hit
GAGTACATGGAAGCAGTGGTATCAA27820.13056686764828046No Hit
GTCTTGCGCCGGTCCAAGAATTTCA25020.11742570196117817No Hit
TATCAACGCAGAGTACTTTTTTTTT24860.11667477820762949No Hit
GCGCAAGACGGACCAGAGCGAAAGC24690.11587692171948398No Hit
CATGTACTCTGCGTTGATACCACTG24630.11559532531190322No Hit
GAATAACGCCGCCGCATCGCCAGTC23530.1104327245062559No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG23520.11038579177165911No Hit
GAATAGGACCGCGGTTCTATTTTGT23420.10991646442569117No Hit
GAACTACGACGGTATCTGATCGTCT22370.10498852729302782No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21350.10020138836415483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCC500.001497182813.30315919
CGTCTTA1750.013.03166615
AACCGTC550.003069029212.0909397
CTAAGAC1501.4551915E-1112.0271453
TAAATCC2800.011.8714034
GCGTTAT2000.011.8680561
AAATCGC1701.8189894E-1211.73753114
CGGTCCA4050.011.729526510
CGAGCCG4550.011.69508515
CGTTATT1950.011.6854712
CGGTTCT4100.011.58757412
CGACCAT4100.011.58648410
ACGGTAT3450.011.5660629
CGCATCG3800.011.50219213
AACGGAC1001.9249837E-611.40270715
CAAATCG1751.8189894E-1211.40217113
GGTTCTA4100.011.35582313
ATCGCCA4050.011.26219816
TTAGAGT2450.011.2414914
CCGACCA4100.011.1227639
Produced by FastQC (version 0.11.5)