##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062553_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2130709
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214414075314835	32.0	32.0	32.0	32.0	32.0
2	30.87335905560074	32.0	32.0	32.0	32.0	32.0
3	30.8615376384105	32.0	32.0	32.0	32.0	32.0
4	30.938291432570097	32.0	32.0	32.0	32.0	32.0
5	30.72676982168846	32.0	32.0	32.0	32.0	32.0
6	34.38036306224829	36.0	36.0	36.0	32.0	36.0
7	34.272458134827424	36.0	36.0	36.0	32.0	36.0
8	34.26705195312922	36.0	36.0	36.0	32.0	36.0
9	34.43646926914938	36.0	36.0	36.0	32.0	36.0
10	34.112198803309134	36.0	36.0	36.0	32.0	36.0
11	34.4139781640759	36.0	36.0	36.0	32.0	36.0
12	34.19721979866795	36.0	36.0	36.0	32.0	36.0
13	34.317430489100104	36.0	36.0	36.0	32.0	36.0
14	34.22177594406369	36.0	36.0	36.0	32.0	36.0
15	34.15562613195889	36.0	36.0	36.0	32.0	36.0
16	34.15814360384267	36.0	36.0	36.0	32.0	36.0
17	34.08444935465143	36.0	36.0	36.0	32.0	36.0
18	34.08711701128591	36.0	36.0	36.0	32.0	36.0
19	34.085011608811904	36.0	36.0	36.0	32.0	36.0
20	34.07751692042414	36.0	36.0	36.0	32.0	36.0
21	34.046255964563905	36.0	36.0	36.0	32.0	36.0
22	34.00556481434114	36.0	36.0	36.0	32.0	36.0
23	33.956909648384645	36.0	36.0	36.0	32.0	36.0
24	33.924657003842384	36.0	36.0	36.0	32.0	36.0
25	33.45918189673015	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	87.0
5	197.0
6	369.0
7	96.0
8	321.0
9	405.0
10	236.0
11	97.0
12	137.0
13	122.0
14	356.0
15	501.0
16	680.0
17	894.0
18	1237.0
19	1661.0
20	2630.0
21	3714.0
22	5706.0
23	8202.0
24	11916.0
25	16778.0
26	23809.0
27	31288.0
28	42819.0
29	57035.0
30	74469.0
31	102655.0
32	149584.0
33	217715.0
34	486233.0
35	888750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.85636370463549	17.05414640557235	10.96380226423596	26.125687625556203
2	16.937573980882544	19.653922007605303	36.49143112467838	26.917072886833772
3	19.28350632076065	23.280787835152385	28.221513396708342	29.214192447378622
4	12.784881010999309	14.909780149990121	35.282835627182976	37.0225032118276
5	14.775885146977147	36.16321831849187	33.60340138322041	15.457495151310566
6	35.11234820760307	34.61472363946776	16.51659985030882	13.756328302620346
7	30.593058310175735	30.170844113728258	20.636740337329886	18.59935723876612
8	28.546095709364767	32.620317841186505	19.414984693247224	19.4186017562015
9	27.008772745765132	14.417951760703323	18.49060291134519	40.082672582186355
10	15.8073269694784	26.57853126989684	31.6650259730801	25.94911578754467
11	37.72658860479639	21.025627531258504	21.706409490724536	19.54137437322057
12	24.42814135719677	23.69877897594138	28.474504743205227	23.398574923656625
13	29.58016370171309	19.088790722786435	25.14990797123684	26.181137604263633
14	23.458900086369827	19.53640361305942	24.741665875648124	32.263030424922626
15	25.422056412015394	26.81365616204711	22.319301592373147	25.44498583356435
16	26.19563230715089	25.593746020902042	23.475679518340616	24.734942153606454
17	24.235315788781186	25.75213761966253	25.20229616722774	24.810250424328544
18	25.35371378918672	24.44343563506352	26.09570562065039	24.107144955099365
19	25.517601457216816	25.524838666624056	24.937543822712808	24.020016053446323
20	25.34370005793925	24.24022281002081	25.310195851587174	25.105881280452763
21	26.510654375662607	24.41699812777732	24.12911569433897	24.943231802221103
22	25.43514586334697	24.561728409568957	24.721963050615628	25.281162676468444
23	24.151573529197506	24.278187159761117	25.328506913226423	26.241732397814953
24	24.544635111243533	25.396150299785607	24.885259984942575	25.173954604028282
25	25.02544750482868	24.572467820537522	24.783378996292136	25.61870567834166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	231.0
1	231.0
2	592.0
3	953.0
4	953.0
5	953.0
6	2591.0
7	4229.0
8	4229.0
9	4229.0
10	4523.0
11	4817.0
12	4817.0
13	4817.0
14	5449.0
15	6081.0
16	6081.0
17	6081.0
18	9804.5
19	13528.0
20	13528.0
21	13528.0
22	21538.5
23	29549.0
24	29549.0
25	29549.0
26	43536.5
27	57524.0
28	57524.0
29	57524.0
30	70778.5
31	84033.0
32	84033.0
33	84033.0
34	105636.5
35	127240.0
36	127240.0
37	127240.0
38	145425.5
39	163611.0
40	163611.0
41	163611.0
42	189545.0
43	215479.0
44	215479.0
45	215479.0
46	253804.5
47	292130.0
48	292130.0
49	292130.0
50	290110.0
51	288090.0
52	288090.0
53	288090.0
54	268116.5
55	248143.0
56	248143.0
57	248143.0
58	229160.0
59	210177.0
60	210177.0
61	210177.0
62	186388.0
63	162599.0
64	162599.0
65	162599.0
66	134158.0
67	105717.0
68	105717.0
69	105717.0
70	79804.5
71	53892.0
72	53892.0
73	53892.0
74	41903.5
75	29915.0
76	29915.0
77	29915.0
78	24543.5
79	19172.0
80	19172.0
81	19172.0
82	13428.5
83	7685.0
84	7685.0
85	7685.0
86	5609.0
87	3533.0
88	3533.0
89	3533.0
90	2405.0
91	1277.0
92	1277.0
93	1277.0
94	746.5
95	216.0
96	216.0
97	216.0
98	552.0
99	888.0
100	888.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008870286838794036
2	0.004270878848308239
3	0.005209533540244116
4	0.014173685848231738
5	0.035293416416788966
6	0.04651033998542269
7	0.06842792704212541
8	0.08964152307987623
9	0.09719769334996003
10	0.11939687681424352
11	0.12052326244456658
12	0.132491109766749
13	0.12061712791376016
14	0.12441867941610045
15	0.11446893968158017
16	0.11263856303230521
17	0.12394935207013252
18	0.13084846405586123
19	0.13220951335916822
20	0.12286989917440626
21	0.1293935492833606
22	0.15037248164812747
23	0.13967181816005844
24	0.13399295727384639
25	0.13136472413642597
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2130709.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.59588699891767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.69877008474204	41.12128735245071
2	12.111101298195344	12.497660280282643
3	3.46441317226418	5.362484116611137
4	1.4916171590357232	3.078452415330152
5	0.8012771000338733	2.0671301354084086
6	0.5072600242428305	1.570351853394079
7	0.33916657773800235	1.2249720293145716
8	0.2368641444254425	0.9776972503896358
9	0.18634782010748308	0.8653302961884303
>10	0.9747143887580847	9.060180918063681
>50	0.08707568144598839	3.1622164172410065
>100	0.08326620846855359	9.46498728758983
>500	0.012570025099713734	4.5652156650754385
>1k	0.005374141165819641	4.454349230169295
>5k	1.8217427680744544E-4	0.5276847524909615
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6028	0.28291052414947326	No Hit
TCCATGTACTCTGCGTTGATACCAC	5200	0.24405021990332795	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3644	0.17102288487071676	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2782	0.13056686764828046	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2502	0.11742570196117817	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2486	0.11667477820762949	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2469	0.11587692171948398	No Hit
CATGTACTCTGCGTTGATACCACTG	2463	0.11559532531190322	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2353	0.1104327245062559	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2352	0.11038579177165911	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2342	0.10991646442569117	No Hit
GAACTACGACGGTATCTGATCGTCT	2237	0.10498852729302782	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2135	0.10020138836415483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.386546919358768E-5
2	0.0	0.0	0.0	0.0	9.386546919358768E-5
3	0.0	0.0	0.0	0.0	9.386546919358768E-5
4	0.0	0.0	0.0	0.0	9.386546919358768E-5
5	0.0	0.0	0.0	0.0	9.386546919358768E-5
6	0.0	0.0	0.0	0.0	2.346636729839692E-4
7	0.0	0.0	0.0	0.0	2.346636729839692E-4
8	0.0	0.0	0.0	0.0	2.346636729839692E-4
9	0.0	0.0	0.0	4.693273459679384E-5	2.346636729839692E-4
10	4.693273459679384E-5	0.0	0.0	4.693273459679384E-5	2.346636729839692E-4
11	4.693273459679384E-5	0.0	0.0	4.693273459679384E-5	2.346636729839692E-4
12	4.693273459679384E-5	0.0	0.0	9.386546919358768E-5	4.693273459679384E-4
13	4.693273459679384E-5	0.0	0.0	9.386546919358768E-5	4.693273459679384E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGCC	50	0.0014971828	13.303159	19
CGTCTTA	175	0.0	13.031666	15
AACCGTC	55	0.0030690292	12.090939	7
CTAAGAC	150	1.4551915E-11	12.027145	3
TAAATCC	280	0.0	11.871403	4
GCGTTAT	200	0.0	11.868056	1
AAATCGC	170	1.8189894E-12	11.737531	14
CGGTCCA	405	0.0	11.7295265	10
CGAGCCG	455	0.0	11.695085	15
CGTTATT	195	0.0	11.685471	2
CGGTTCT	410	0.0	11.587574	12
CGACCAT	410	0.0	11.586484	10
ACGGTAT	345	0.0	11.566062	9
CGCATCG	380	0.0	11.502192	13
AACGGAC	100	1.9249837E-6	11.402707	15
CAAATCG	175	1.8189894E-12	11.402171	13
GGTTCTA	410	0.0	11.355823	13
ATCGCCA	405	0.0	11.262198	16
TTAGAGT	245	0.0	11.241491	4
CCGACCA	410	0.0	11.122763	9
>>END_MODULE