FastQCFastQC Report
Thu 2 Feb 2017
SRR4062553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130709
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC27110.1272346434919081No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23870.11202843748254689No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23280.10925940614133606No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22980.10785142410343224No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT22730.1066781057385124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTT851.4315986E-418.11745332
CTAGCGG8050.018.03707529
GTATAGG2200.018.0044561
ATACGAA7850.017.93611340
CGCAATA7750.017.8836836
TTCGTCG750.001291401317.59981235
TCTAGCG8200.017.43883728
CGCCGGT8500.017.3409927
CGAGCCG8150.017.00595315
CGGTCCA8700.016.94234810
TAGCGGC8450.016.92289730
CGAATGC8400.016.76172443
TACGTTG800.001989162516.49982533
TCGAACG5100.016.39198144
CGTCGTA5350.016.03721210
AATACGA8950.015.97748339
CAATACG8900.015.82005538
GGCGTTA6000.015.76649942
GCAATAC8800.015.74983137
ACGATTA1401.5876903E-615.71411832
Produced by FastQC (version 0.11.5)