FastQCFastQC Report
Thu 2 Feb 2017
SRR4062552_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062552_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944820
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT64710.6848923604496094No Hit
GTCCTACAGTGGACATTTCTAAATT58180.6157786668360111No Hit
CTGTAGGACGTGGAATATGGCAAGA56080.593552211003154No Hit
CTTTAGGACGTGAAATATGGCGAGG51170.5415846404606168No Hit
GTATCAACGCAGAGTACTTTTTTTT44380.46971909993437905No Hit
GTACATGGAAGCAGTGGTATCAACG43210.45733578882750153No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38040.4026163713723249No Hit
TCCATGTACTCTGCGTTGATACCAC37550.39743019834465826No Hit
TATCAACGCAGAGTACTTTTTTTTT32160.34038229504032513No Hit
GTCCTACAGTGTGCATTTCTCATTT31070.32884570606041363No Hit
TTGTAGAACAGTGTATATCAATGAG28330.29984547321182875No Hit
GATATACACTGTTCTACAAATCCCG27230.28820304396604646No Hit
CTGAAGGACCTGGAATATGGCGAGA26760.2832285514701213No Hit
GTCCTTCAGTGTGCATTTCTCATTT25310.26788171291886287No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25010.2647065049427404No Hit
CTGTAGGACCTGGAATATGGCGAGA23870.25264071463347515No Hit
ATTTAGAAATGTCCACTGTAGGACG23750.2513706314430262No Hit
GTGTATATCAATGAGTTACAATGAA22610.23930484113376094No Hit
TTTCTAAATTTTCCACCTTTTTCAG19000.20109650515442093No Hit
GAGTACATGGAAGCAGTGGTATCAA18780.1987680193052645No Hit
GTTCTACAGTGTGGTTTTTATCATT18730.19823881797591075No Hit
GTGTATATCAATGAGTTACAATGAG18570.1965453737219788No Hit
GTACATGGGGTGGTATCAACGCAAA18390.19464024893630533No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17760.18797231218644822No Hit
GAATATGGCAAGAAAACTGAAAATC17720.18754895112296524No Hit
GATATACACTGTTCTACAATGCCGG16090.1702969877860333No Hit
GGTATCAACGCAGAGTACTTTTTTT15420.16320568997269322No Hit
CATGTACTCTGCGTTGATACCACTG15420.16320568997269322No Hit
GGAATATGGCGAGAAAACTGAAAAT14990.15865455854025104No Hit
ATTCCAGGTCCTTCAGTGTGCATTT13110.13875658855655046No Hit
CTGTAGAACATATTAGATGAGTGAG12930.13685146377087698No Hit
GTACATGGGAAGCAGTGGTATCAAC12810.13558138058042804No Hit
GAAATATGGCGAGGAAAACTGAAAA12800.13547554031455727No Hit
GTTCTACAAATCCCGTTTCCAACGA12430.1315594504773396No Hit
CCATATTCCAGGTCCTTCAGTGTGC12380.13103024914798586No Hit
GACCTGGAATATGGCGAGAAAACTG11970.1266907982472852No Hit
CCCATGTACTCTGCGTTGATACCAC11890.1258440761203192No Hit
CTGTAGGACATGGAATATGGCAAGA11850.12542071505683622No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11790.12478567346161173No Hit
ACCTGGAATATGGCGAGAAAACTGA11760.12446815266399949No Hit
GCCATATTCCACGTCCTACAGTGGA11650.12330390973942128No Hit
GTCCACTGTAGGACGTGGAATATGG11600.12277470841006752No Hit
ATGTTCTACAGTGTGGTTTTTATCA11580.12256302787832603No Hit
CCATATTTCACGTCCTAAAGTGTGT11540.12213966681484305No Hit
GAATAGGACCGCGGTTCTATTTTGT11480.12150462521961856No Hit
GATTAAGAGGGACGGCCGGGGGCAT11400.12065790309265256No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11390.12055206282678183No Hit
GCTTCCATGTACTCTGCGTTGATAC11210.11864693804110836No Hit
GCGCAAGACGGACCAGAGCGAAAGC11200.11854109777523761No Hit
GTACATGGGTGGTATCAACGCAAAA11020.11663597298956416No Hit
TTCCAGGTCCTTCAGTGTGCATTTC10800.1143074871404077No Hit
ACAGTGGACATTTCTAAATTTTCCA10610.1122965220888635No Hit
ATACACACTTTAGGACGTGAAATAT10470.11081475836667302No Hit
CTATTGGAGCTGGAATTACCGCGGC10290.10890963358099956No Hit
GTCCTATTCCATTATTCCTAGCTGC10270.10869795304925806No Hit
CATGGAAGCAGTGGTATCAACGCAG10230.10827459198577508No Hit
CCACTGTAGGACGTGGAATATGGCA10190.10785123092229207No Hit
GTATCTGATCGTCTTCGAACCTCCG10160.10753371012467983No Hit
GAACTACGACGGTATCTGATCGTCT10160.10753371012467983No Hit
GAATAACGCCGCCGCATCGCCAGTC10070.10658114773184309No Hit
CCTAAAGTGTGTATTTCTCATTTTC10060.10647530746597236No Hit
GCGTTGATACCACTGCTTCCATGTA9780.1035117800215914No Hit
GTAGAACAGTGTATATCAATGAGTT9750.10319425922397917No Hit
GGGTAGGCACACGCTGAGCCAGTCA9710.10277089816049619No Hit
GTATCAACGCAGAGTACATGGGAAG9630.1019241760335302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTGT250.00603113918.99957513
ATGACCT250.006036677418.9965575
AAGTACC504.566351E-617.09694
ACGCGCG402.760145E-416.6246313
GGACAAT350.002168906216.28621311
CCAACGA2300.014.87396219
TAAGCTC456.754565E-414.77901412
ATACGCG456.7681447E-414.77514
GCTCGTA653.3676642E-614.6158329
ACGTTAA400.00527414214.2511928
TCCGGTG400.00527414214.25119212
GCACCGG400.005275996414.2504386
GAAGTAC400.005285275614.2466643
GCGTTAT602.5743979E-514.2451541
CCATTAA602.5743979E-514.2451541
TCCAACG3950.014.19406918
ACGCGTA551.95836E-413.8186046
TACGCGT551.9618834E-413.8156775
CCAACGT1600.013.66028819
CTCGTAG707.253022E-613.57184510
Produced by FastQC (version 0.11.5)