##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062552_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	944820
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046098727800004	32.0	32.0	32.0	32.0	32.0
2	30.8576638936517	32.0	32.0	32.0	32.0	32.0
3	30.855901653224954	32.0	32.0	32.0	32.0	32.0
4	30.87057852289325	32.0	32.0	32.0	32.0	32.0
5	30.80374251180119	32.0	32.0	32.0	32.0	32.0
6	34.41212082724752	36.0	36.0	36.0	32.0	36.0
7	34.21497216401008	36.0	36.0	36.0	32.0	36.0
8	34.21372430727546	36.0	36.0	36.0	32.0	36.0
9	34.27009377447556	36.0	36.0	36.0	32.0	36.0
10	34.072960987278	36.0	36.0	36.0	32.0	36.0
11	34.34538642281069	36.0	36.0	36.0	32.0	36.0
12	34.163033170339325	36.0	36.0	36.0	32.0	36.0
13	34.250919751910416	36.0	36.0	36.0	32.0	36.0
14	34.20498401811985	36.0	36.0	36.0	32.0	36.0
15	34.176281196418365	36.0	36.0	36.0	32.0	36.0
16	34.19116657141043	36.0	36.0	36.0	32.0	36.0
17	34.123759022882666	36.0	36.0	36.0	32.0	36.0
18	34.18916619038547	36.0	36.0	36.0	32.0	36.0
19	34.07277153320209	36.0	36.0	36.0	32.0	36.0
20	34.0107449037912	36.0	36.0	36.0	32.0	36.0
21	33.928498549988355	36.0	36.0	36.0	32.0	36.0
22	33.908366673017085	36.0	36.0	36.0	32.0	36.0
23	33.93476852733854	36.0	36.0	36.0	32.0	36.0
24	33.90617895472153	36.0	36.0	36.0	32.0	36.0
25	33.4807402468195	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	28.0
5	97.0
6	180.0
7	34.0
8	138.0
9	178.0
10	113.0
11	29.0
12	68.0
13	42.0
14	280.0
15	305.0
16	455.0
17	669.0
18	917.0
19	1242.0
20	1831.0
21	2422.0
22	3641.0
23	4851.0
24	6376.0
25	8407.0
26	11186.0
27	13903.0
28	18320.0
29	23781.0
30	31066.0
31	41879.0
32	60074.0
33	88650.0
34	203635.0
35	420017.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.68840269067314	19.187823169214973	12.416578018407083	24.7071961217048
2	12.679841361740321	21.31265009933414	45.04985758755111	20.957650951374433
3	19.65934818480975	26.862529464624984	29.60762169273807	23.87050065782719
4	10.888014049186975	17.24031710175181	38.46631825981469	33.40535058924653
5	11.287964212922523	40.043304481325606	35.21924878900977	13.449482516742107
6	29.965955959824857	38.07955610383697	19.217040021601818	12.737447914736357
7	26.30563857932966	31.961481676047	22.721023879073137	19.011855865550206
8	24.170577454791996	40.21695604733201	20.4736379334089	15.138828564467092
9	27.12611772682968	14.659596558884605	20.776793660211045	37.437492054074674
10	16.222829052846187	28.647950992952214	33.36671254984365	21.762507404357947
11	35.264179074427496	20.18836554529685	27.100117940398246	17.44733743987742
12	25.912952979092985	23.601385816891156	32.98988192577253	17.495779278243333
13	30.983255816910766	21.71148631249662	26.572184585517622	20.733073285074987
14	20.496476447835533	24.840036030307846	26.41413659725534	28.24935092460128
15	21.532861797663514	36.199528464330186	23.417944846221094	18.84966489178521
16	20.040053404539385	26.82412528874478	33.263080933307904	19.87274037340793
17	19.281775098767316	29.739029621716938	30.437607033250252	20.541588246265494
18	20.29659633817027	25.622422030327307	36.48656382342842	17.594417808074
19	24.554099927825078	25.33859153974231	26.98695658164298	23.12035195078963
20	23.895935993217822	27.52058496264505	29.541991204366024	19.0414878397711
21	23.397093843360892	25.87286968925892	25.610571619030864	25.11946484834932
22	23.176175398389375	30.77432711337441	26.878257634140056	19.171239854096154
23	19.698349187842815	29.668936643790246	29.423673123294204	21.209041045072734
24	23.74357599273368	27.62558940829713	29.763833938683522	18.867000660285672
25	22.04278454584475	28.413198878720596	29.948864148203885	19.59515242723077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	403.0
1	403.0
2	863.5
3	1324.0
4	1324.0
5	1324.0
6	3268.5
7	5213.0
8	5213.0
9	5213.0
10	4805.5
11	4398.0
12	4398.0
13	4398.0
14	3826.5
15	3255.0
16	3255.0
17	3255.0
18	5697.5
19	8140.0
20	8140.0
21	8140.0
22	16550.5
23	24961.0
24	24961.0
25	24961.0
26	43008.0
27	61055.0
28	61055.0
29	61055.0
30	84177.0
31	107299.0
32	107299.0
33	107299.0
34	108608.5
35	109918.0
36	109918.0
37	109918.0
38	106404.5
39	102891.0
40	102891.0
41	102891.0
42	105825.5
43	108760.0
44	108760.0
45	108760.0
46	119769.5
47	130779.0
48	130779.0
49	130779.0
50	112236.0
51	93693.0
52	93693.0
53	93693.0
54	71780.5
55	49868.0
56	49868.0
57	49868.0
58	44961.0
59	40054.0
60	40054.0
61	40054.0
62	37064.5
63	34075.0
64	34075.0
65	34075.0
66	29313.0
67	24551.0
68	24551.0
69	24551.0
70	18678.5
71	12806.0
72	12806.0
73	12806.0
74	10832.0
75	8858.0
76	8858.0
77	8858.0
78	7990.5
79	7123.0
80	7123.0
81	7123.0
82	4984.5
83	2846.0
84	2846.0
85	2846.0
86	2171.5
87	1497.0
88	1497.0
89	1497.0
90	1033.5
91	570.0
92	570.0
93	570.0
94	330.0
95	90.0
96	90.0
97	90.0
98	241.5
99	393.0
100	393.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007938019940306091
2	0.003069367710251688
3	0.004551131432442158
4	0.014711796956033953
5	0.03651489172540801
6	0.04815732097119028
7	0.06837281175250312
8	0.08996422599013569
9	0.099489849918503
10	0.11843525750936687
11	0.11885861857284985
12	0.13346457526301306
13	0.12161046548548929
14	0.12383311106877501
15	0.11589509112846892
16	0.1143074871404077
17	0.12573823585444846
18	0.13208865180669332
19	0.1353697000486865
20	0.12383311106877501
21	0.13092440888211512
22	0.15442094790542113
23	0.14235515759615588
24	0.136428102707394
25	0.13282953366778857
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	944820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.424175797889816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75336588921323	24.88664628671609
2	12.712382052281399	8.243770209462227
3	3.9598790594169513	3.8518744428275338
4	1.7226310456531342	2.2341956743663993
5	0.9337547043030723	1.5138113342214716
6	0.6056298935966014	1.1782230083060103
7	0.4197362451143778	0.9526721260234329
8	0.3152968616168682	0.8178592695670617
9	0.2393230889039997	0.6983868516405569
>10	1.74289522786727	11.442777229663669
>50	0.2671102616087163	6.110873591468687
>100	0.27856718116880946	18.660903642891526
>500	0.029460688960273332	6.529373956610788
>1k	0.01865843634150644	10.435981245875283
>5k	0.0013093639537899257	2.4426511303592546
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	6471	0.6848923604496094	No Hit
GTCCTACAGTGGACATTTCTAAATT	5818	0.6157786668360111	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5608	0.593552211003154	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5117	0.5415846404606168	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4438	0.46971909993437905	No Hit
GTACATGGAAGCAGTGGTATCAACG	4321	0.45733578882750153	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3804	0.4026163713723249	No Hit
TCCATGTACTCTGCGTTGATACCAC	3755	0.39743019834465826	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3216	0.34038229504032513	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3107	0.32884570606041363	No Hit
TTGTAGAACAGTGTATATCAATGAG	2833	0.29984547321182875	No Hit
GATATACACTGTTCTACAAATCCCG	2723	0.28820304396604646	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2676	0.2832285514701213	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2531	0.26788171291886287	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2501	0.2647065049427404	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2387	0.25264071463347515	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2375	0.2513706314430262	No Hit
GTGTATATCAATGAGTTACAATGAA	2261	0.23930484113376094	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1900	0.20109650515442093	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1878	0.1987680193052645	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1873	0.19823881797591075	No Hit
GTGTATATCAATGAGTTACAATGAG	1857	0.1965453737219788	No Hit
GTACATGGGGTGGTATCAACGCAAA	1839	0.19464024893630533	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1776	0.18797231218644822	No Hit
GAATATGGCAAGAAAACTGAAAATC	1772	0.18754895112296524	No Hit
GATATACACTGTTCTACAATGCCGG	1609	0.1702969877860333	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1542	0.16320568997269322	No Hit
CATGTACTCTGCGTTGATACCACTG	1542	0.16320568997269322	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1499	0.15865455854025104	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1311	0.13875658855655046	No Hit
CTGTAGAACATATTAGATGAGTGAG	1293	0.13685146377087698	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1281	0.13558138058042804	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1280	0.13547554031455727	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1243	0.1315594504773396	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1238	0.13103024914798586	No Hit
GACCTGGAATATGGCGAGAAAACTG	1197	0.1266907982472852	No Hit
CCCATGTACTCTGCGTTGATACCAC	1189	0.1258440761203192	No Hit
CTGTAGGACATGGAATATGGCAAGA	1185	0.12542071505683622	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1179	0.12478567346161173	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1176	0.12446815266399949	No Hit
GCCATATTCCACGTCCTACAGTGGA	1165	0.12330390973942128	No Hit
GTCCACTGTAGGACGTGGAATATGG	1160	0.12277470841006752	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	1158	0.12256302787832603	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1154	0.12213966681484305	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1148	0.12150462521961856	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1140	0.12065790309265256	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1139	0.12055206282678183	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1121	0.11864693804110836	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1120	0.11854109777523761	No Hit
GTACATGGGTGGTATCAACGCAAAA	1102	0.11663597298956416	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1080	0.1143074871404077	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1061	0.1122965220888635	No Hit
ATACACACTTTAGGACGTGAAATAT	1047	0.11081475836667302	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1029	0.10890963358099956	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1027	0.10869795304925806	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1023	0.10827459198577508	No Hit
CCACTGTAGGACGTGGAATATGGCA	1019	0.10785123092229207	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1016	0.10753371012467983	No Hit
GAACTACGACGGTATCTGATCGTCT	1016	0.10753371012467983	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1007	0.10658114773184309	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	1006	0.10647530746597236	No Hit
GCGTTGATACCACTGCTTCCATGTA	978	0.1035117800215914	No Hit
GTAGAACAGTGTATATCAATGAGTT	975	0.10319425922397917	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	971	0.10277089816049619	No Hit
GTATCAACGCAGAGTACATGGGAAG	963	0.1019241760335302	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.0584026587074787E-4	0.0	0.0	0.0
6	0.0	1.0584026587074787E-4	0.0	0.0	0.0
7	0.0	1.0584026587074787E-4	0.0	0.0	0.0
8	0.0	1.0584026587074787E-4	0.0	1.0584026587074787E-4	0.0
9	0.0	1.0584026587074787E-4	0.0	2.1168053174149573E-4	0.0
10	0.0	1.0584026587074787E-4	0.0	2.1168053174149573E-4	0.0
11	0.0	1.0584026587074787E-4	0.0	2.1168053174149573E-4	0.0
12	0.0	1.0584026587074787E-4	0.0	2.1168053174149573E-4	0.0
13	0.0	2.1168053174149573E-4	0.0	3.175207976122436E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTGT	25	0.006031139	18.999575	13
ATGACCT	25	0.0060366774	18.996557	5
AAGTACC	50	4.566351E-6	17.0969	4
ACGCGCG	40	2.760145E-4	16.62463	13
GGACAAT	35	0.0021689062	16.286213	11
CCAACGA	230	0.0	14.873962	19
TAAGCTC	45	6.754565E-4	14.779014	12
ATACGCG	45	6.7681447E-4	14.7751	4
GCTCGTA	65	3.3676642E-6	14.615832	9
ACGTTAA	40	0.005274142	14.251192	8
TCCGGTG	40	0.005274142	14.251192	12
GCACCGG	40	0.0052759964	14.250438	6
GAAGTAC	40	0.0052852756	14.246664	3
GCGTTAT	60	2.5743979E-5	14.245154	1
CCATTAA	60	2.5743979E-5	14.245154	1
TCCAACG	395	0.0	14.194069	18
ACGCGTA	55	1.95836E-4	13.818604	6
TACGCGT	55	1.9618834E-4	13.815677	5
CCAACGT	160	0.0	13.660288	19
CTCGTAG	70	7.253022E-6	13.571845	10
>>END_MODULE