FastQCFastQC Report
Thu 2 Feb 2017
SRR4062552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944820
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA31280.33106835164369935No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA24560.25994369297855674No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA24100.25507504074850235No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC22450.23761139687982893No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA19750.20903452509472703No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16590.1755890010795707No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15840.16765098113926463No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA14770.1563260726910946No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG14570.15420926737367965No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA12600.13335873499714232No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC12000.12700831904489746No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA11960.12658495798141445No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11880.12573823585444846No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11600.12277470841006752No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC11500.12171630575136005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.11684765352130565No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10770.11398996634279544No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10610.1122965220888635No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10240.10838043225164583No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10230.10827459198577508No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10210.10806291145403359No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA9880.1045701826802989No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9800.10372346055333291No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT9740.10308841895810843No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG9490.10044241231133971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA207.8560493E-443.9996839
TCCGCGA250.002352617235.19974526
CGCTAAG300.005744589529.3331240
CGCCTTA407.0312846E-427.49980233
GGACGTT757.009476E-826.399816
GCCTTAT508.339946E-526.39980934
CGTGTAA701.1319025E-625.1426759
TCTTCGC450.001398046524.4442744
GACGTTA450.001398046524.444277
GTGATCG450.001398046524.444279
GACGTGT752.0636035E-623.4664977
TATAGGA2700.022.814652
ACTGTTC13150.022.7526878
TAGGACA8100.022.5430474
TATGTCG1700.021.99984216
TCGCCTT500.00258013321.9998432
TTCGTCT500.00258013321.9998444
TACGGCG500.00258013321.9998416
CTACGTG500.00258013321.9998444
GAGTTCG500.00258013321.9998444
Produced by FastQC (version 0.11.5)