##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062552_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	944820
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.155434897652462	32.0	32.0	32.0	32.0	32.0
2	31.469964649351198	32.0	32.0	32.0	32.0	32.0
3	31.539401155775703	32.0	32.0	32.0	32.0	32.0
4	31.64045955843441	32.0	32.0	32.0	32.0	32.0
5	31.591653436633433	32.0	32.0	32.0	32.0	32.0
6	35.19456298554222	36.0	36.0	36.0	36.0	36.0
7	35.200943036768905	36.0	36.0	36.0	36.0	36.0
8	35.15534281662116	36.0	36.0	36.0	36.0	36.0
9	35.236510658114774	36.0	36.0	36.0	36.0	36.0
10	35.158833428589574	36.0	36.0	36.0	36.0	36.0
11	35.26035223640482	36.0	36.0	36.0	36.0	36.0
12	35.179996189750426	36.0	36.0	36.0	36.0	36.0
13	35.19840922080396	36.0	36.0	36.0	36.0	36.0
14	35.1606115450562	36.0	36.0	36.0	36.0	36.0
15	35.13849410469719	36.0	36.0	36.0	36.0	36.0
16	35.15540737918334	36.0	36.0	36.0	36.0	36.0
17	35.149569230117905	36.0	36.0	36.0	36.0	36.0
18	35.16724561292098	36.0	36.0	36.0	36.0	36.0
19	35.100913401494466	36.0	36.0	36.0	36.0	36.0
20	35.057637433585235	36.0	36.0	36.0	36.0	36.0
21	35.013967739886965	36.0	36.0	36.0	36.0	36.0
22	35.00109756355708	36.0	36.0	36.0	36.0	36.0
23	34.98672974746513	36.0	36.0	36.0	36.0	36.0
24	34.972531275798566	36.0	36.0	36.0	36.0	36.0
25	34.92831650473106	36.0	36.0	36.0	36.0	36.0
26	34.896199276052585	36.0	36.0	36.0	36.0	36.0
27	34.856176837916216	36.0	36.0	36.0	32.0	36.0
28	34.793565970237715	36.0	36.0	36.0	32.0	36.0
29	34.74767680616414	36.0	36.0	36.0	32.0	36.0
30	34.699593573379055	36.0	36.0	36.0	32.0	36.0
31	34.69479265891916	36.0	36.0	36.0	32.0	36.0
32	34.637739463601534	36.0	36.0	36.0	32.0	36.0
33	34.618274380305245	36.0	36.0	36.0	32.0	36.0
34	34.549987299168095	36.0	36.0	36.0	32.0	36.0
35	34.491450223322964	36.0	36.0	36.0	32.0	36.0
36	34.445281640947485	36.0	36.0	36.0	32.0	36.0
37	34.34600347156072	36.0	36.0	36.0	32.0	36.0
38	34.28482568108211	36.0	36.0	36.0	32.0	36.0
39	34.12267627696281	36.0	36.0	36.0	32.0	36.0
40	34.13627463432188	36.0	36.0	36.0	32.0	36.0
41	34.09531127622193	36.0	36.0	36.0	32.0	36.0
42	34.020311805423255	36.0	36.0	36.0	32.0	36.0
43	34.016611629728416	36.0	36.0	36.0	32.0	36.0
44	33.908366673017085	36.0	36.0	36.0	32.0	36.0
45	33.815605088799984	36.0	36.0	36.0	32.0	36.0
46	33.723456319722274	36.0	36.0	36.0	27.0	36.0
47	33.68889735611016	36.0	36.0	36.0	27.0	36.0
48	33.59171376558498	36.0	36.0	36.0	21.0	36.0
49	33.53462670138227	36.0	36.0	36.0	21.0	36.0
50	33.04941682013505	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	29.0
22	108.0
23	281.0
24	801.0
25	1955.0
26	4201.0
27	8088.0
28	13854.0
29	19789.0
30	27241.0
31	36666.0
32	52351.0
33	78688.0
34	157929.0
35	542834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.928995698922456	20.021341305270646	12.912230706284994	25.137432289521904
2	12.27091203339617	21.718729988706354	44.4738928363141	21.536465141583374
3	19.259960944735212	26.90727814439864	30.072448045384547	23.760312865481602
4	10.708211219738848	17.65093134437674	38.595833902226566	33.04502353365784
5	11.03173091170805	39.96539023306027	35.317097436548764	13.685781418682923
6	30.37362009292874	38.15486711613975	18.70735915157545	12.76415363935606
7	26.236214305370336	31.913380326411378	22.798839990686055	19.051565377532228
8	24.134755826506638	40.17791748692873	20.531000613873545	15.156326072691096
9	26.828602273448908	14.419571981964818	20.834232975593235	37.91759276899304
10	16.363222624415233	28.516860354353213	33.28030736013209	21.83960966109947
11	35.22046527380876	19.942211214834572	27.273237230371922	17.564086280984736
12	25.7442687496031	23.546601469062892	33.08132766029509	17.627802121038926
13	31.190808831311784	21.41519019495777	26.742342456764252	20.651658516966194
14	20.602336953070427	24.795622446603584	26.489278381067294	28.1127622192587
15	21.65428335555979	36.03882220952139	23.384983383078257	18.921911051840564
16	20.14172011600093	26.864164602781482	32.98384877542813	20.010266505789463
17	19.38559725662031	29.750746173874386	30.21443237865413	20.649224190851168
18	20.515865455854023	25.189877436972118	36.592790161088885	17.701466946084967
19	24.751381215469614	24.9757625791156	27.418450075146588	22.8544061302682
20	24.107449037911984	27.302237463220507	29.65612497618594	18.93418852268157
21	23.432823183251834	25.40991934971741	26.155352342245088	25.001905124785672
22	23.307931669524354	30.170402828051905	27.355686797485234	19.165978704938507
23	19.698037721470758	29.222074045850004	29.67623462670138	21.40365360597786
24	23.81988103554116	27.29874473444677	29.887280116847652	18.99409411316441
25	22.299908977371352	27.923519823881797	29.97597425964734	19.80059693909951
26	17.622721788277133	30.607734806629832	32.26445248830465	19.505090916788383
27	20.331280032175442	29.067441417412844	30.33636036493724	20.26491818547448
28	18.600050803327616	30.106263626934233	28.548506593848565	22.745178975889587
29	20.467602294616963	26.842361508012107	28.32719459791283	24.3628415994581
30	18.95694417984378	28.254905696323107	30.417010647530745	22.371139476302364
31	22.713426896128365	26.24203551999323	28.795114413327404	22.249423170551005
32	20.129230964628185	29.601193878199023	28.986367773755852	21.283207383416947
33	18.892804978726108	29.12364259859021	31.879193920535126	20.104358502148557
34	20.60868736902267	29.511864693804114	31.44048601850088	18.43896191867234
35	20.361021146885122	30.044241231133974	30.568150547194172	19.026587074786733
36	18.272898541521137	30.077898435680872	30.92705488876188	20.72214813403611
37	20.05662454224085	29.563408903283168	27.777248576448425	22.602717978027563
38	20.843229398192246	31.059355221100315	27.410723735738024	20.686691644969414
39	23.029889291081897	26.43519400520734	28.877352299908978	21.657564403801782
40	24.898816705827564	27.274507313562374	29.34304946974027	18.483626510869797
41	22.097965750089966	25.714950995956904	32.50100548252578	19.686077771427364
42	22.60853919265045	30.827988399906857	29.574627971465468	16.988844435977224
43	18.932389238161768	30.045828835122034	30.615143625240787	20.406638301475414
44	18.21966088778815	30.606993924768737	29.289494295209668	21.883850892233443
45	18.81734086916026	30.957431045066784	28.92318113503101	21.30204695074194
46	19.6334959394339	26.92716806076084	29.523750051597187	23.915585948208072
47	23.102520165237998	27.109425191491702	29.075304370466725	20.712750272803575
48	20.119070299104592	29.2228149277111	27.029910459135074	23.628204314049235
49	21.42101140958066	28.15308736055545	28.695836243940644	21.730064985923246
50	19.37162634152537	32.36373065769141	26.116297284138778	22.148345716644442
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22.0
1	32.5
2	43.0
3	135.5
4	228.0
5	261.0
6	294.0
7	304.5
8	315.0
9	405.5
10	496.0
11	732.5
12	969.0
13	1720.0
14	2471.0
15	3546.0
16	4621.0
17	5651.5
18	6682.0
19	7104.0
20	7526.0
21	8035.0
22	8544.0
23	8880.5
24	9217.0
25	9934.0
26	10651.0
27	16624.0
28	22597.0
29	28492.0
30	34387.0
31	41255.0
32	48123.0
33	60437.5
34	72752.0
35	86246.0
36	99740.0
37	97732.0
38	95724.0
39	83931.0
40	72138.0
41	62732.5
42	53327.0
43	46350.0
44	39373.0
45	41297.5
46	43222.0
47	45687.5
48	48153.0
49	46615.5
50	45078.0
51	37783.0
52	30488.0
53	27995.5
54	25503.0
55	27766.5
56	30030.0
57	29923.5
58	29817.0
59	26975.0
60	24133.0
61	21073.5
62	18014.0
63	16078.5
64	14143.0
65	12560.0
66	10977.0
67	10102.5
68	9228.0
69	9384.5
70	9541.0
71	7260.5
72	4980.0
73	4702.5
74	4425.0
75	3089.5
76	1754.0
77	1740.5
78	1727.0
79	1471.5
80	1216.0
81	998.5
82	781.0
83	710.0
84	639.0
85	534.0
86	429.0
87	328.5
88	228.0
89	136.0
90	44.0
91	30.0
92	16.0
93	10.5
94	5.0
95	3.5
96	2.0
97	3.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018310365995639382
2	0.004339450900700662
3	5.292013293537394E-4
4	3.1752079761224357E-4
5	0.0
6	0.0010584026587074787
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.0584026587074788E-4
47	1.0584026587074788E-4
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	944820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.354123538251244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.08581066729595	35.86867627127289
2	12.072908641819234	10.951123800141813
3	3.625563765059841	4.93302800688994
4	1.5207456836482982	2.758883504257891
5	0.8005891157419855	1.815500882937067
6	0.5159384298808767	1.4039961172169197
7	0.3436632844575069	1.0910582941192861
8	0.2536335554302208	0.9202662085142502
9	0.18884848298793575	0.7708551684701559
>10	1.245784292608738	11.105297797005695
>50	0.16540902018826822	5.299301089542134
>100	0.16048766714799112	14.954859328542296
>500	0.015697333865095097	4.74225635347265
>1k	0.004920059868164134	3.3848971776170482
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	3128	0.33106835164369935	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	2456	0.25994369297855674	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	2410	0.25507504074850235	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2245	0.23761139687982893	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1975	0.20903452509472703	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1659	0.1755890010795707	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1584	0.16765098113926463	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1477	0.1563260726910946	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1457	0.15420926737367965	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1260	0.13335873499714232	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	1200	0.12700831904489746	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	1196	0.12658495798141445	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1188	0.12573823585444846	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1160	0.12277470841006752	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	1150	0.12171630575136005	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1104	0.11684765352130565	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1077	0.11398996634279544	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1061	0.1122965220888635	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1024	0.10838043225164583	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1023	0.10827459198577508	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1021	0.10806291145403359	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	988	0.1045701826802989	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	980	0.10372346055333291	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	974	0.10308841895810843	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	949	0.10044241231133971	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.0584026587074787E-4	0.0
14	0.0	0.0	0.0	1.0584026587074787E-4	0.0
15	0.0	0.0	0.0	2.1168053174149573E-4	0.0
16	0.0	0.0	0.0	4.2336106348299146E-4	0.0
17	0.0	0.0	0.0	6.350415952244872E-4	0.0
18	0.0	0.0	0.0	8.467221269659829E-4	0.0
19	0.0	0.0	0.0	8.467221269659829E-4	0.0
20	0.0	0.0	0.0	0.0012700831904489745	0.0
21	0.0	0.0	0.0	0.0016934442539319658	0.0
22	0.0	0.0	0.0	0.002540166380897949	0.0
23	0.0	0.0	0.0	0.005715374357020385	0.0
24	0.0	0.0	0.0	0.011113227916428527	0.0
25	0.0	0.0	0.0	0.013759234563197222	0.0
26	0.0	0.0	0.0	0.018733727059122372	0.0
27	0.0	0.0	0.0	0.02296733769395229	0.0
28	0.0	0.0	0.0	0.032916322685802586	0.0
29	0.0	0.0	0.0	0.051015008149700475	0.0
30	0.0	0.0	0.0	0.086471497216401	0.0
31	0.0	0.0	0.0	0.15780783641328508	0.0
32	0.0	0.0	0.0	0.24660781947884253	0.0
33	0.0	0.0	0.0	0.33064499058021635	0.0
34	0.0	0.0	0.0	0.42463114667344043	0.0
35	0.0	0.0	0.0	0.5294130098854808	0.0
36	0.0	0.0	0.0	0.6713448064181538	0.0
37	0.0	0.0	0.0	0.8933976842149828	0.0
38	0.0	0.0	0.0	1.1529180161300565	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTAA	20	7.8560493E-4	43.99968	39
TCCGCGA	25	0.0023526172	35.199745	26
CGCTAAG	30	0.0057445895	29.33312	40
CGCCTTA	40	7.0312846E-4	27.499802	33
GGACGTT	75	7.009476E-8	26.39981	6
GCCTTAT	50	8.339946E-5	26.399809	34
CGTGTAA	70	1.1319025E-6	25.142675	9
TCTTCGC	45	0.0013980465	24.44427	44
GACGTTA	45	0.0013980465	24.44427	7
GTGATCG	45	0.0013980465	24.44427	9
GACGTGT	75	2.0636035E-6	23.466497	7
TATAGGA	270	0.0	22.81465	2
ACTGTTC	1315	0.0	22.752687	8
TAGGACA	810	0.0	22.543047	4
TATGTCG	170	0.0	21.999842	16
TCGCCTT	50	0.002580133	21.99984	32
TTCGTCT	50	0.002580133	21.99984	44
TACGGCG	50	0.002580133	21.99984	16
CTACGTG	50	0.002580133	21.99984	44
GAGTTCG	50	0.002580133	21.99984	44
>>END_MODULE