FastQCFastQC Report
Thu 2 Feb 2017
SRR4062551_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062551_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152558
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26870.12482822762499314No Hit
GCGCAAGACGGACCAGAGCGAAAGC24560.11409680947040685No Hit
GATTAAGAGGGACGGCCGGGGGCAT24150.1121920988888569No Hit
GAATAACGCCGCCGCATCGCCAGTC23940.11121651542025812No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23770.11042675737424962No Hit
GAATAGGACCGCGGTTCTATTTTGT23300.10824330865881429No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG23280.1081503959475192No Hit
GAACTACGACGGTATCTGATCGTCT22780.1058275781651412No Hit
GTATCTGATCGTCTTCGAACCTCCG21640.10053155362131938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG3300.012.66762913
AAGACGG4750.011.9983995
CGCCAGT3650.011.97629718
TCGACGC1052.7196802E-711.76252314
TCGCCAG3900.011.69537317
GTGTTAG1552.5465852E-1111.6379231
GTATCAA23950.011.6149061
ATCGCCA3850.011.59909216
CAAGACG4800.011.4771014
CGTTATT2000.011.394772
TTTTCAA3950.011.30754919
CTCGACG1104.966096E-711.22812513
CGACCAT3550.010.97266410
CATCGCC4250.010.95428315
GCGCGCT2000.010.92633815
CCGACCA3500.010.8579669
ACGGTAT3600.010.8202669
GCATCGC4050.010.7906914
CGGTCCA3700.010.78460210
TAATACC1158.8410525E-710.7371984
Produced by FastQC (version 0.11.5)