##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062551_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2152558
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.213616543665722	32.0	32.0	32.0	32.0	32.0
2	30.825609809352407	32.0	32.0	32.0	32.0	32.0
3	30.79734065237731	32.0	32.0	32.0	32.0	32.0
4	30.900601052329367	32.0	32.0	32.0	32.0	32.0
5	30.655875010104257	32.0	32.0	32.0	32.0	32.0
6	34.31846668010804	36.0	36.0	36.0	32.0	36.0
7	34.20553220865593	36.0	36.0	36.0	32.0	36.0
8	34.21011652183123	36.0	36.0	36.0	32.0	36.0
9	34.38333694144362	36.0	36.0	36.0	32.0	36.0
10	34.02278591331802	36.0	36.0	36.0	32.0	36.0
11	34.361036032478566	36.0	36.0	36.0	32.0	36.0
12	34.1015415147931	36.0	36.0	36.0	32.0	36.0
13	34.24605330030596	36.0	36.0	36.0	32.0	36.0
14	34.149502127236524	36.0	36.0	36.0	32.0	36.0
15	34.074027273597274	36.0	36.0	36.0	32.0	36.0
16	34.08600558033744	36.0	36.0	36.0	32.0	36.0
17	34.00312233166307	36.0	36.0	36.0	32.0	36.0
18	34.00706136605843	36.0	36.0	36.0	32.0	36.0
19	34.00214674819448	36.0	36.0	36.0	32.0	36.0
20	34.01267561663844	36.0	36.0	36.0	32.0	36.0
21	33.99279787118396	36.0	36.0	36.0	32.0	36.0
22	33.93945157343031	36.0	36.0	36.0	32.0	36.0
23	33.901283496193834	36.0	36.0	36.0	32.0	36.0
24	33.86303644315275	36.0	36.0	36.0	32.0	36.0
25	33.416299584029794	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	9.0
4	69.0
5	216.0
6	355.0
7	80.0
8	315.0
9	358.0
10	214.0
11	78.0
12	141.0
13	139.0
14	268.0
15	385.0
16	575.0
17	732.0
18	1113.0
19	1461.0
20	2398.0
21	3591.0
22	5615.0
23	8758.0
24	12639.0
25	18260.0
26	25482.0
27	33758.0
28	45888.0
29	61543.0
30	79257.0
31	109086.0
32	156503.0
33	226215.0
34	496134.0
35	860921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44061587494802	17.252071539246	10.753373583257641	26.553939002548336
2	17.850029849082112	19.892589762113268	35.46893256430329	26.788447824501333
3	19.20384673457299	23.367751077248283	28.112521632074706	29.31588055610402
4	13.143618341026073	14.887563968059414	35.24973446343959	36.719083227474925
5	15.242912910121758	35.81434148155033	33.18296310065991	15.759782507668
6	34.76746304562431	34.77996553217833	16.538279918124978	13.91429150407237
7	30.69840328305978	29.97405125783064	20.707114845730032	18.620430613379547
8	28.695237763131892	31.687089537492298	19.52231172484337	20.095360974532436
9	27.677764344834	13.955640286431695	18.42355621686971	39.943039151864596
10	16.303075909899317	25.968919059702493	31.008293459738212	26.71971157065998
11	37.98158515389656	20.946713033221975	21.788901327511624	19.28280048536984
12	24.80828681018836	23.469620332064085	28.115624241217663	23.606468616529895
13	29.521489352993925	18.952945181778606	25.089066121944775	26.436499343282694
14	23.797413129514222	19.363807906099083	24.332805739133427	32.505973225253264
15	25.603474936693555	26.572476287696112	21.728771703821177	26.09527707178916
16	26.50503795323209	25.269476783932127	22.96419346707728	25.2612917957585
17	24.802317723243416	25.21119364700487	24.599623914148612	25.386864715603096
18	25.584816810425025	24.3224813759126	25.379179721513147	24.713522092149233
19	26.038660173843798	24.61935911205422	24.75518184664032	24.586798867461663
20	26.054836277651965	23.904676724929804	24.292012410741403	25.748474586676824
21	27.23003144361546	23.772582655776137	23.841144622025414	25.156241278582993
22	26.481428237787785	23.85554231183291	24.219356987800577	25.443672462578732
23	24.94782929876809	23.679543968062134	24.997046063728735	26.37558066944104
24	25.152735280244936	24.60133463702171	24.48657966934568	25.759350413387676
25	25.143684042093984	24.214086896982867	24.681839155738803	25.960389905184343
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	144.0
1	144.0
2	447.0
3	750.0
4	750.0
5	750.0
6	1999.0
7	3248.0
8	3248.0
9	3248.0
10	3586.5
11	3925.0
12	3925.0
13	3925.0
14	5010.0
15	6095.0
16	6095.0
17	6095.0
18	9721.5
19	13348.0
20	13348.0
21	13348.0
22	21495.0
23	29642.0
24	29642.0
25	29642.0
26	43404.5
27	57167.0
28	57167.0
29	57167.0
30	69810.0
31	82453.0
32	82453.0
33	82453.0
34	104727.5
35	127002.0
36	127002.0
37	127002.0
38	146166.0
39	165330.0
40	165330.0
41	165330.0
42	188949.5
43	212569.0
44	212569.0
45	212569.0
46	240305.5
47	268042.0
48	268042.0
49	268042.0
50	274736.5
51	281431.0
52	281431.0
53	281431.0
54	265109.5
55	248788.0
56	248788.0
57	248788.0
58	231252.5
59	213717.0
60	213717.0
61	213717.0
62	192519.0
63	171321.0
64	171321.0
65	171321.0
66	145418.0
67	119515.0
68	119515.0
69	119515.0
70	92148.0
71	64781.0
72	64781.0
73	64781.0
74	52180.0
75	39579.0
76	39579.0
77	39579.0
78	32009.0
79	24439.0
80	24439.0
81	24439.0
82	17689.5
83	10940.0
84	10940.0
85	10940.0
86	8125.5
87	5311.0
88	5311.0
89	5311.0
90	3566.0
91	1821.0
92	1821.0
93	1821.0
94	1087.0
95	353.0
96	353.0
97	353.0
98	600.0
99	847.0
100	847.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008036949527027843
2	0.0029267504057962665
3	0.0038558775187474623
4	0.014494382962038655
5	0.03521391758085032
6	0.04580596666849395
7	0.06476015977269833
8	0.0828316821195991
9	0.09096154435792207
10	0.11288894422357028
11	0.11358578955828368
12	0.12691876362913335
13	0.11474719844947268
14	0.11832433783433478
15	0.10698898705633018
16	0.10592049087643632
17	0.11693064716490797
18	0.12329516788862366
19	0.12529279118146874
20	0.11544404378418607
21	0.1244101204241651
22	0.14457217877520606
23	0.13398012968756243
24	0.128219541587265
25	0.12552507295970652
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2152558.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.33387734430278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.98716282221477	41.573850827786515
2	11.44574647471477	11.751090912939882
3	3.0618890246835146	4.7153590690491125
4	1.3391033616721206	2.7496547087768066
5	0.7295040387129529	1.8724135422732116
6	0.46343289038735447	1.4273884291476102
7	0.3159109181446909	1.135185262663595
8	0.24050025146996465	0.9876648328186513
9	0.18619027619628434	0.8602081920865726
>10	1.0185646007840727	9.469645897228983
>50	0.09694904007684496	3.4716239256041193
>100	0.0950132930750936	10.46630765822559
>500	0.014594182202023543	5.203655226964737
>1k	0.005438825665350388	4.315951514434724
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2687	0.12482822762499314	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2456	0.11409680947040685	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2415	0.1121920988888569	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2394	0.11121651542025812	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2377	0.11042675737424962	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2330	0.10824330865881429	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2328	0.1081503959475192	No Hit
GAACTACGACGGTATCTGATCGTCT	2278	0.1058275781651412	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2164	0.10053155362131938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6456355647559786E-5
2	0.0	0.0	0.0	0.0	4.6456355647559786E-5
3	0.0	0.0	0.0	0.0	4.6456355647559786E-5
4	0.0	0.0	0.0	0.0	4.6456355647559786E-5
5	0.0	0.0	0.0	4.6456355647559786E-5	4.6456355647559786E-5
6	0.0	0.0	0.0	4.6456355647559786E-5	4.6456355647559786E-5
7	0.0	0.0	0.0	9.291271129511957E-5	4.6456355647559786E-5
8	0.0	0.0	0.0	9.291271129511957E-5	4.6456355647559786E-5
9	0.0	0.0	0.0	9.291271129511957E-5	4.6456355647559786E-5
10	0.0	0.0	0.0	9.291271129511957E-5	4.6456355647559786E-5
11	0.0	0.0	0.0	9.291271129511957E-5	4.6456355647559786E-5
12	0.0	0.0	0.0	9.291271129511957E-5	1.8582542259023915E-4
13	0.0	0.0	0.0	9.291271129511957E-5	2.3228177823779895E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	330	0.0	12.667629	13
AAGACGG	475	0.0	11.998399	5
CGCCAGT	365	0.0	11.976297	18
TCGACGC	105	2.7196802E-7	11.762523	14
TCGCCAG	390	0.0	11.695373	17
GTGTTAG	155	2.5465852E-11	11.637923	1
GTATCAA	2395	0.0	11.614906	1
ATCGCCA	385	0.0	11.599092	16
CAAGACG	480	0.0	11.477101	4
CGTTATT	200	0.0	11.39477	2
TTTTCAA	395	0.0	11.307549	19
CTCGACG	110	4.966096E-7	11.228125	13
CGACCAT	355	0.0	10.972664	10
CATCGCC	425	0.0	10.954283	15
GCGCGCT	200	0.0	10.926338	15
CCGACCA	350	0.0	10.857966	9
ACGGTAT	360	0.0	10.820266	9
GCATCGC	405	0.0	10.79069	14
CGGTCCA	370	0.0	10.784602	10
TAATACC	115	8.8410525E-7	10.737198	4
>>END_MODULE