FastQCFastQC Report
Thu 2 Feb 2017
SRR4062551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152558
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24760.11502593658335802No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT24040.11168107897673372No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23870.1108913209307252No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23600.1096369993282411No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG21800.10127485531168035No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA21590.10029927184308157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30000.024.5727881
TAGGTCG550.004484893819.9998745
AACGCAG41050.017.6856386
CGTACTA2500.016.7198933
TCAACGC43450.016.7087554
GGTATCA14100.016.6991961
TATCAAC44700.016.5371912
CAACGCA44050.016.4811675
GTCTAGA3350.016.4221
ATCAACG44850.016.187193
GTTTCGA850.002981077915.52931325
ATACCGT6350.015.2439996
CGTCGTA5800.015.172317510
GTCTTAG3650.015.0722491
GTACTAT2051.8062565E-915.0281351
TCTAGCG7200.014.97212728
CGCAATA7100.014.87314636
ACGCAGA49000.014.8162347
ACCGTCG6250.014.7839068
CTAGCGG7400.014.56747529
Produced by FastQC (version 0.11.5)