##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062551_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2152558
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32886918726464	32.0	32.0	32.0	32.0	32.0
2	31.424897261769484	32.0	32.0	32.0	32.0	32.0
3	31.498711765257895	32.0	32.0	32.0	32.0	32.0
4	31.594149844046015	32.0	32.0	32.0	32.0	32.0
5	31.536456160530864	32.0	32.0	32.0	32.0	32.0
6	35.12332443539268	36.0	36.0	36.0	36.0	36.0
7	35.13895374712319	36.0	36.0	36.0	36.0	36.0
8	35.08219615917434	36.0	36.0	36.0	36.0	36.0
9	35.21339448228573	36.0	36.0	36.0	36.0	36.0
10	35.07850148520969	36.0	36.0	36.0	36.0	36.0
11	35.21566805633112	36.0	36.0	36.0	36.0	36.0
12	35.121130766279	36.0	36.0	36.0	36.0	36.0
13	35.17189873629421	36.0	36.0	36.0	36.0	36.0
14	35.13537382035699	36.0	36.0	36.0	36.0	36.0
15	35.0929224671298	36.0	36.0	36.0	36.0	36.0
16	35.107647273615854	36.0	36.0	36.0	36.0	36.0
17	35.07819766064375	36.0	36.0	36.0	36.0	36.0
18	35.09209043380016	36.0	36.0	36.0	36.0	36.0
19	35.076757049055125	36.0	36.0	36.0	36.0	36.0
20	35.0775407677749	36.0	36.0	36.0	36.0	36.0
21	35.07781207289188	36.0	36.0	36.0	36.0	36.0
22	35.05866415678462	36.0	36.0	36.0	36.0	36.0
23	35.01719582004294	36.0	36.0	36.0	36.0	36.0
24	34.9922232060646	36.0	36.0	36.0	36.0	36.0
25	34.966680572602456	36.0	36.0	36.0	32.0	36.0
26	34.924045716770465	36.0	36.0	36.0	32.0	36.0
27	34.913967010412726	36.0	36.0	36.0	32.0	36.0
28	34.89704296005032	36.0	36.0	36.0	32.0	36.0
29	34.87409026841553	36.0	36.0	36.0	32.0	36.0
30	34.86568073891621	36.0	36.0	36.0	32.0	36.0
31	34.86946832559216	36.0	36.0	36.0	32.0	36.0
32	34.8350218670066	36.0	36.0	36.0	32.0	36.0
33	34.81356692827789	36.0	36.0	36.0	32.0	36.0
34	34.81198508936809	36.0	36.0	36.0	32.0	36.0
35	34.78910812159301	36.0	36.0	36.0	32.0	36.0
36	34.765989116205	36.0	36.0	36.0	32.0	36.0
37	34.76669896931929	36.0	36.0	36.0	32.0	36.0
38	34.73448938425817	36.0	36.0	36.0	32.0	36.0
39	34.737549928968235	36.0	36.0	36.0	32.0	36.0
40	34.73447823473281	36.0	36.0	36.0	32.0	36.0
41	34.70250139601349	36.0	36.0	36.0	32.0	36.0
42	34.68342827463883	36.0	36.0	36.0	32.0	36.0
43	34.67461457484537	36.0	36.0	36.0	32.0	36.0
44	34.64702042871783	36.0	36.0	36.0	32.0	36.0
45	34.61683866358072	36.0	36.0	36.0	32.0	36.0
46	34.62856796425462	36.0	36.0	36.0	32.0	36.0
47	34.613782764506226	36.0	36.0	36.0	32.0	36.0
48	34.5708761389937	36.0	36.0	36.0	32.0	36.0
49	34.56568138930519	36.0	36.0	36.0	32.0	36.0
50	34.04745516729398	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	16.0
21	43.0
22	158.0
23	476.0
24	1265.0
25	2929.0
26	6287.0
27	11550.0
28	19552.0
29	30376.0
30	44730.0
31	65762.0
32	98083.0
33	165865.0
34	376338.0
35	1329127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08841611870839	17.792459177263417	11.196655379159868	26.92246932486832
2	17.14362171157468	20.440618562640015	35.1737117382138	27.24204798757151
3	18.49122782566683	23.679226218267214	28.432297582628934	29.397248373437023
4	12.807483575324941	15.508507568218963	35.451143153667694	36.23286570278841
5	14.976832215438563	36.025928221214016	33.13095396268068	15.86628560066674
6	34.987946836933176	35.01052474115548	16.26426740043307	13.737261021478275
7	30.685366287629083	30.07023739673328	20.736500822045596	18.507895493592038
8	28.718635557618217	31.817229462448616	19.42220345384582	20.041931526087346
9	27.815650031265125	13.831961786860097	18.345010912597942	40.00737726927683
10	16.282395178201934	26.007475756750804	31.046782479264206	26.663346585783053
11	38.23980584959848	20.821413406746764	21.733862688020487	19.204918055634273
12	24.800771918805438	23.49762468653574	28.05030108364095	23.65130231101787
13	29.56059720574312	18.907736748556832	25.08838321661948	26.443282829080562
14	23.890459629891506	19.227124193633806	24.31493135144326	32.56748482503143
15	25.687437922694766	26.576751938856003	21.644712941532816	26.09109719691641
16	26.67365989673681	25.3450081252166	22.842636528260797	25.138695449785793
17	24.95458891235451	25.24549861141953	24.462894844180738	25.33701763204522
18	25.818630671043476	24.28259772791256	25.268029943908598	24.63074165713537
19	26.15966677785221	24.59552773955452	24.74799749878981	24.496807983803457
20	26.251092885766607	23.794016235567174	24.215654119424425	25.739236759241795
21	27.388809035575346	23.81004367826558	23.764098342530144	25.037048943628932
22	26.534058548015892	23.81784834601437	24.1808583090444	25.467234796925332
23	25.02394825133632	23.596019247797273	24.917423827836462	26.462608673029948
24	25.308679255100213	24.59664269209006	24.433441514700185	25.661236538109545
25	25.409350177788475	24.017146111742402	24.661495764574056	25.91200794589507
26	25.333719230794244	24.732062968802698	24.85271012441941	25.081507675983644
27	26.220245865616633	24.182159087002532	24.01203591262117	25.585559134759666
28	25.08842967297513	24.170777279868883	25.116582224497552	25.624210822658437
29	25.144409581530443	24.46359168951545	24.81972611190964	25.57227261704447
30	25.0661306222643	24.64658327441119	25.07584000059464	25.211446102729866
31	25.750293371885913	24.248173568377716	24.03145466928185	25.97007839045452
32	25.480010294728412	24.22183281472555	24.1066210527196	26.191535837826436
33	25.167544846642926	23.781798214031863	24.765093437668114	26.2855635016571
34	25.75400988033772	24.0647174199255	24.850573132059626	25.330699567677158
35	26.372622712140625	23.882422680364478	24.667720916230827	25.077233691264066
36	25.17836917750881	24.406403915713305	24.522916455677386	25.892310451100503
37	26.313112120556102	24.2066415864288	24.131475202991044	25.34877109002405
38	25.267751205774715	23.85859056991728	24.937353604409264	25.936304619898742
39	25.707414155623216	23.984905400922997	24.16910485106557	26.138575592388218
40	25.94726831983157	23.972362184898156	24.953520416174616	25.126849079095663
41	24.90204677411712	24.487748994452183	25.204338280315792	25.405865951114908
42	26.21680809529871	24.41560227413152	24.694804971573355	24.672784658996413
43	25.320664994857285	23.455442315607755	25.01897742128203	26.204915268252932
44	25.049917354143304	23.99131637800236	24.734757437430257	26.224008830424083
45	25.058976343494578	24.429074617269315	24.852292017218584	25.659657022017523
46	24.92589046422464	24.011164396575793	24.998594694588807	26.064350444610756
47	24.950361383990582	23.844003274243946	25.468303293105226	25.737332048660242
48	25.749782351973792	24.463312951381567	24.641380162578663	25.145524534065984
49	24.592786814571316	24.6310668516249	24.897215313129774	25.878931020674006
50	24.564866544827133	24.754826583070002	24.915704942677504	25.764601929425364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	12.5
2	13.0
3	64.0
4	115.0
5	156.5
6	198.0
7	200.5
8	203.0
9	274.5
10	346.0
11	502.0
12	658.0
13	1180.0
14	1702.0
15	2283.0
16	2864.0
17	3474.5
18	4085.0
19	4434.5
20	4784.0
21	5833.5
22	6883.0
23	7711.0
24	8539.0
25	10825.5
26	13112.0
27	16457.0
28	19802.0
29	22568.5
30	25335.0
31	27124.5
32	28914.0
33	33277.0
34	37640.0
35	44163.0
36	50686.0
37	62932.0
38	75178.0
39	82136.5
40	89095.0
41	99290.5
42	109486.0
43	111464.5
44	113443.0
45	124131.0
46	134819.0
47	144077.5
48	153336.0
49	158722.5
50	164109.0
51	158206.5
52	152304.0
53	149208.5
54	146113.0
55	148611.0
56	151109.0
57	149592.5
58	148076.0
59	136662.0
60	125248.0
61	110377.5
62	95507.0
63	85716.5
64	75926.0
65	65487.0
66	55048.0
67	50766.0
68	46484.0
69	42839.5
70	39195.0
71	31103.0
72	23011.0
73	20680.0
74	18349.0
75	14037.5
76	9726.0
77	8652.5
78	7579.0
79	6482.0
80	5385.0
81	4277.0
82	3169.0
83	2749.0
84	2329.0
85	1929.0
86	1529.0
87	1158.5
88	788.0
89	512.0
90	236.0
91	148.0
92	60.0
93	37.0
94	14.0
95	10.5
96	7.0
97	5.5
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015144771941104489
2	0.0039023338743950226
3	6.039326234182772E-4
4	1.8582542259023915E-4
5	0.0
6	5.110199121231576E-4
7	4.6456355647559786E-5
8	4.6456355647559786E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	4.6456355647559786E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2152558.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.15930271188829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53354497225018	44.15799943325456
2	11.350640572556795	12.294855574858891
3	2.9437465531838134	4.782937820428797
4	1.2621472448406632	2.7342805880120507
5	0.6876834349780919	1.8622227662464814
6	0.4419940964715064	1.4362855240600723
7	0.2978215799487853	1.129086637180537
8	0.20967246870432743	0.9084571762325274
9	0.1677687998053457	0.8177617092841115
>10	0.9229662516229914	9.047933289896989
>50	0.08294736879024145	3.12521150209029
>100	0.08217071699719171	9.457886242528375
>500	0.012585751112900227	4.666448650567823
>1k	0.004310188737294597	3.5786330853585664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2476	0.11502593658335802	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2404	0.11168107897673372	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2387	0.1108913209307252	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2360	0.1096369993282411	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2180	0.10127485531168035	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2159	0.10029927184308157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6456355647559786E-5
2	0.0	0.0	0.0	0.0	4.6456355647559786E-5
3	0.0	0.0	0.0	0.0	4.6456355647559786E-5
4	0.0	0.0	0.0	0.0	9.291271129511957E-5
5	0.0	0.0	0.0	4.6456355647559786E-5	9.291271129511957E-5
6	0.0	0.0	0.0	4.6456355647559786E-5	1.3936906694267937E-4
7	0.0	0.0	0.0	4.6456355647559786E-5	1.3936906694267937E-4
8	0.0	0.0	0.0	4.6456355647559786E-5	1.3936906694267937E-4
9	0.0	0.0	0.0	4.6456355647559786E-5	1.3936906694267937E-4
10	0.0	0.0	0.0	4.6456355647559786E-5	1.3936906694267937E-4
11	0.0	0.0	0.0	4.6456355647559786E-5	1.3936906694267937E-4
12	0.0	0.0	0.0	4.6456355647559786E-5	2.3228177823779895E-4
13	0.0	0.0	0.0	4.6456355647559786E-5	2.7873813388535875E-4
14	0.0	0.0	0.0	4.6456355647559786E-5	2.7873813388535875E-4
15	0.0	0.0	0.0	4.6456355647559786E-5	2.7873813388535875E-4
16	0.0	0.0	0.0	4.6456355647559786E-5	3.251944895329185E-4
17	0.0	0.0	0.0	4.6456355647559786E-5	3.251944895329185E-4
18	0.0	0.0	0.0	1.3936906694267937E-4	3.251944895329185E-4
19	0.0	0.0	0.0	1.3936906694267937E-4	3.251944895329185E-4
20	0.0	0.0	0.0	2.7873813388535875E-4	5.574762677707175E-4
21	0.0	0.0	0.0	2.7873813388535875E-4	5.574762677707175E-4
22	0.0	0.0	0.0	3.716508451804783E-4	6.039326234182772E-4
23	0.0	0.0	0.0	6.968453347133968E-4	6.50388979065837E-4
24	0.0	0.0	0.0	0.001300777958131674	6.50388979065837E-4
25	0.0	0.0	0.0	0.0014866033807219132	6.50388979065837E-4
26	0.0	0.0	0.0	0.0018117978702548317	6.968453347133968E-4
27	0.0	0.0	0.0	0.0028802940501487066	6.968453347133968E-4
28	0.0	0.0	0.0	0.008269231305265643	6.968453347133968E-4
29	0.0	0.0	0.0	0.01955812572762267	6.968453347133968E-4
30	0.0	0.0	0.0	0.044272906932124476	6.968453347133968E-4
31	0.0	0.0	0.0	0.10094966082214742	6.968453347133968E-4
32	0.0	0.0	0.0	0.1638051100132958	6.968453347133968E-4
33	0.0	0.0	0.0	0.2318636710369709	6.968453347133968E-4
34	0.0	0.0	0.0	0.3091670468345104	6.968453347133968E-4
35	0.0	0.0	0.0	0.3959010628285045	6.968453347133968E-4
36	0.0	0.0	0.0	0.5171521510686355	6.968453347133968E-4
37	0.0	0.0	0.0	0.6921996991486409	6.968453347133968E-4
38	0.0	0.0	0.0	0.9392545984823638	6.968453347133968E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3000	0.0	24.572788	1
TAGGTCG	55	0.0044848938	19.999874	5
AACGCAG	4105	0.0	17.685638	6
CGTACTA	250	0.0	16.719893	3
TCAACGC	4345	0.0	16.708755	4
GGTATCA	1410	0.0	16.699196	1
TATCAAC	4470	0.0	16.537191	2
CAACGCA	4405	0.0	16.481167	5
GTCTAGA	335	0.0	16.422	1
ATCAACG	4485	0.0	16.18719	3
GTTTCGA	85	0.0029810779	15.529313	25
ATACCGT	635	0.0	15.243999	6
CGTCGTA	580	0.0	15.1723175	10
GTCTTAG	365	0.0	15.072249	1
GTACTAT	205	1.8062565E-9	15.028135	1
TCTAGCG	720	0.0	14.972127	28
CGCAATA	710	0.0	14.873146	36
ACGCAGA	4900	0.0	14.816234	7
ACCGTCG	625	0.0	14.783906	8
CTAGCGG	740	0.0	14.567475	29
>>END_MODULE