FastQCFastQC Report
Thu 2 Feb 2017
SRR4062550_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062550_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229396
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32350.2631373454932341No Hit
TATCAACGCAGAGTACTTTTTTTTT22290.1813085450090939No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21920.17829893703900127No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16440.13372420277925093No Hit
GTACATGGAAGCAGTGGTATCAACG14020.11403973983972618No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13460.10948465750661299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTC1055.456968E-1215.38322710
GGCCCGT400.005274513714.2515286
CGCATCG1800.013.72313513
ACAATAC1051.378794E-913.56514553
ATCGCCA1800.013.19585916
AGAACCG1450.013.1032515
GCATCGC1850.012.83869214
TCTAGTA604.111182E-412.6592562
TCGCCAG1850.012.32614817
TCGCGTA853.934889E-612.2959389
GATTGTG550.00306535112.0922057
AACCGCG1209.986252E-911.8762747
CCGCATC2250.011.82397212
GTTATAT1052.744382E-711.7531091
CAAGACG1950.011.6907034
TTTGCGC907.4446016E-611.61282917
GTATTAC907.519231E-611.6024291
CGTCTTA752.0702899E-411.40168815
TTCGAAG752.0844361E-411.3933322
CTATTAT752.0875913E-411.3914771
Produced by FastQC (version 0.11.5)