##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062550_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1229396 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.145647130786173 32.0 32.0 32.0 32.0 32.0 2 30.82616748387013 32.0 32.0 32.0 32.0 32.0 3 30.822167145492582 32.0 32.0 32.0 32.0 32.0 4 30.88539982235179 32.0 32.0 32.0 32.0 32.0 5 30.695670068879352 32.0 32.0 32.0 32.0 32.0 6 34.34480590468816 36.0 36.0 36.0 32.0 36.0 7 34.21595808022801 36.0 36.0 36.0 32.0 36.0 8 34.20189507693209 36.0 36.0 36.0 32.0 36.0 9 34.35543714149062 36.0 36.0 36.0 32.0 36.0 10 34.03469671285737 36.0 36.0 36.0 32.0 36.0 11 34.36093252296249 36.0 36.0 36.0 32.0 36.0 12 34.134512394704394 36.0 36.0 36.0 32.0 36.0 13 34.26167809233152 36.0 36.0 36.0 32.0 36.0 14 34.17077898415157 36.0 36.0 36.0 32.0 36.0 15 34.11491008592837 36.0 36.0 36.0 32.0 36.0 16 34.1195009581941 36.0 36.0 36.0 32.0 36.0 17 34.04435511421869 36.0 36.0 36.0 32.0 36.0 18 34.057633179219714 36.0 36.0 36.0 32.0 36.0 19 34.032523287858425 36.0 36.0 36.0 32.0 36.0 20 34.026959580151555 36.0 36.0 36.0 32.0 36.0 21 33.9983617971752 36.0 36.0 36.0 32.0 36.0 22 33.962223726122424 36.0 36.0 36.0 32.0 36.0 23 33.93358445936053 36.0 36.0 36.0 32.0 36.0 24 33.90075126322194 36.0 36.0 36.0 32.0 36.0 25 33.460434229491554 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 56.0 5 123.0 6 211.0 7 45.0 8 189.0 9 223.0 10 132.0 11 49.0 12 90.0 13 74.0 14 242.0 15 320.0 16 538.0 17 608.0 18 787.0 19 1122.0 20 1750.0 21 2494.0 22 3833.0 23 5516.0 24 7529.0 25 10728.0 26 14555.0 27 18844.0 28 25280.0 29 33561.0 30 43263.0 31 59003.0 32 84622.0 33 121734.0 34 274950.0 35 516919.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.72243454266502 17.467301838320832 11.066613301835067 25.743650317179085 2 16.81910469906097 20.209508749324847 37.138671430151824 25.832715121462364 3 19.373154482523 24.022109604418667 28.20560793277639 28.39912798028194 4 12.700912705224443 15.328143520638985 35.85811293984801 36.11283083428856 5 14.546771253743001 36.5796283036063 33.52053769040489 15.353062752245803 6 33.86639475136188 35.18490186472485 17.147021436930242 13.801681946983027 7 30.032118694284726 30.491515325159046 20.913453711963847 18.562912268592378 8 27.953262650908446 32.850312339464516 19.85433656459968 19.342088445027358 9 27.515003676114375 14.192406930798674 18.838803809480385 39.45378558360657 10 15.980295309752746 26.745721054358917 31.733216280067854 25.54076735582048 11 37.15368859968808 21.29285246008445 22.382594744707447 19.170864195520014 12 24.810166584269094 23.842327700162976 28.896298787981955 22.45120692758597 13 29.636928970595662 19.782981544310108 25.249732473121085 25.330357011973152 14 23.26617405489312 20.076115115764853 25.389432329133943 31.268278500208087 15 24.994075631586536 27.599331910399123 22.590614334609956 24.81597812340439 16 25.20836505802463 25.90233165284081 24.491597401656172 24.39770588747839 17 23.60209952805411 26.109072844176417 25.652030507494533 24.63679712027494 18 24.40861449850428 24.919838708232444 27.017999946246935 23.653546847016337 19 25.16655969923146 25.192297041508795 25.713396775339064 23.927746483920675 20 25.41063510096383 24.55495697992125 25.660245212412914 24.37416270670201 21 26.393108290152906 24.401650439731746 24.662932055377368 24.54230921473798 22 25.485295519290034 24.994643807059745 25.139485039725436 24.38057563392478 23 24.032089377174806 24.739674968396088 26.00244685980894 25.22578879462017 24 24.456832044934192 25.388617073005847 25.821033743812876 24.33351713824709 25 24.425754976844498 25.11585895261878 25.715149056920346 24.74323701361638 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 253.0 1 253.0 2 586.0 3 919.0 4 919.0 5 919.0 6 2259.5 7 3600.0 8 3600.0 9 3600.0 10 3766.0 11 3932.0 12 3932.0 13 3932.0 14 4090.0 15 4248.0 16 4248.0 17 4248.0 18 6691.0 19 9134.0 20 9134.0 21 9134.0 22 14582.0 23 20030.0 24 20030.0 25 20030.0 26 29425.5 27 38821.0 28 38821.0 29 38821.0 30 48213.0 31 57605.0 32 57605.0 33 57605.0 34 68707.0 35 79809.0 36 79809.0 37 79809.0 38 89988.0 39 100167.0 40 100167.0 41 100167.0 42 113417.5 43 126668.0 44 126668.0 45 126668.0 46 144253.5 47 161839.0 48 161839.0 49 161839.0 50 161813.0 51 161787.0 52 161787.0 53 161787.0 54 148876.0 55 135965.0 56 135965.0 57 135965.0 58 125247.5 59 114530.0 60 114530.0 61 114530.0 62 100857.5 63 87185.0 64 87185.0 65 87185.0 66 72294.0 67 57403.0 68 57403.0 69 57403.0 70 43723.5 71 30044.0 72 30044.0 73 30044.0 74 23581.0 75 17118.0 76 17118.0 77 17118.0 78 13947.5 79 10777.0 80 10777.0 81 10777.0 82 7497.0 83 4217.0 84 4217.0 85 4217.0 86 3115.5 87 2014.0 88 2014.0 89 2014.0 90 1344.0 91 674.0 92 674.0 93 674.0 94 400.0 95 126.0 96 126.0 97 126.0 98 328.5 99 531.0 100 531.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009923572225710836 2 0.003578993261731777 3 0.0053684898925976665 4 0.015698765898050752 5 0.035464569593523974 6 0.0473403199619976 7 0.06735014592531617 8 0.08825472020406769 9 0.09736488487029403 10 0.11916420746447848 11 0.12127906711913818 12 0.13087727632105522 13 0.12054700031560214 14 0.12355660828569477 15 0.1152598511789529 16 0.11249426547670564 17 0.12209247467862268 18 0.12681023852363274 19 0.13079593556510677 20 0.12046565955965369 21 0.13030789102941606 22 0.1500736947248893 23 0.13827928511236412 24 0.13380554353519938 25 0.13095861707700368 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1229396.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.30227419464023 #Duplication Level Percentage of deduplicated Percentage of total 1 81.24553414323273 46.55553874565527 2 11.969089651137153 13.717121140993838 3 3.102146983784274 5.332802311706479 4 1.2228784288796375 2.8029486015348737 5 0.6089415377663729 1.7446867482797286 6 0.37217726071248436 1.279596206541413 7 0.24443543798506204 0.9804694543214901 8 0.17763927605828622 0.8143307603543447 9 0.12754535313396875 0.6577774917781403 >10 0.7492821309034716 8.186107474967026 >50 0.08378907755837976 3.399694542719552 >100 0.08799729805893376 10.260719975039214 >500 0.006692346284932676 2.6403464631164044 >1k 0.0018510745043430806 1.6278600829924408 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3235 0.2631373454932341 No Hit TATCAACGCAGAGTACTTTTTTTTT 2229 0.1813085450090939 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2192 0.17829893703900127 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1644 0.13372420277925093 No Hit GTACATGGAAGCAGTGGTATCAACG 1402 0.11403973983972618 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1346 0.10948465750661299 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.134075594844949E-5 2 0.0 0.0 0.0 0.0 8.134075594844949E-5 3 0.0 0.0 0.0 0.0 8.134075594844949E-5 4 0.0 0.0 0.0 8.134075594844949E-5 8.134075594844949E-5 5 0.0 0.0 0.0 8.134075594844949E-5 8.134075594844949E-5 6 0.0 0.0 0.0 8.134075594844949E-5 8.134075594844949E-5 7 0.0 0.0 0.0 8.134075594844949E-5 8.134075594844949E-5 8 0.0 0.0 0.0 8.134075594844949E-5 8.134075594844949E-5 9 0.0 0.0 0.0 1.6268151189689897E-4 8.134075594844949E-5 10 0.0 0.0 0.0 1.6268151189689897E-4 8.134075594844949E-5 11 0.0 0.0 0.0 1.6268151189689897E-4 8.134075594844949E-5 12 0.0 0.0 0.0 4.067037797422474E-4 8.134075594844949E-5 13 0.0 0.0 0.0 4.067037797422474E-4 8.134075594844949E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGTC 105 5.456968E-12 15.383227 10 GGCCCGT 40 0.0052745137 14.251528 6 CGCATCG 180 0.0 13.723135 13 ACAATAC 105 1.378794E-9 13.5651455 3 ATCGCCA 180 0.0 13.195859 16 AGAACCG 145 0.0 13.103251 5 GCATCGC 185 0.0 12.838692 14 TCTAGTA 60 4.111182E-4 12.659256 2 TCGCCAG 185 0.0 12.326148 17 TCGCGTA 85 3.934889E-6 12.295938 9 GATTGTG 55 0.003065351 12.092205 7 AACCGCG 120 9.986252E-9 11.876274 7 CCGCATC 225 0.0 11.823972 12 GTTATAT 105 2.744382E-7 11.753109 1 CAAGACG 195 0.0 11.690703 4 TTTGCGC 90 7.4446016E-6 11.612829 17 GTATTAC 90 7.519231E-6 11.602429 1 CGTCTTA 75 2.0702899E-4 11.401688 15 TTCGAAG 75 2.0844361E-4 11.393332 2 CTATTAT 75 2.0875913E-4 11.391477 1 >>END_MODULE