FastQCFastQC Report
Thu 2 Feb 2017
SRR4062550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229396
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT2750.020.7999026
TACCGTC2900.020.4826647
ACCGTCG2900.019.7240478
CCGTCGT2850.019.2981579
TAGGACG5900.018.6439824
CGACGGT3700.018.4323467
GACGGTA3650.018.0821088
CGTCGTA3100.017.74185410
TACGACG3650.017.479375
GTGTATA3150.017.4623681
GTCCTAA4800.017.4187131
ACGGTAT3700.017.2431649
ACGACGG3850.016.5713526
ACTATAC800.001988437716.4999243
CGCTTAC800.001988437716.49992418
AGGACGT6500.016.2460785
TGTAGGA6450.016.0322382
TATGGCG6750.015.97029616
CTACGAC4050.015.7530154
GACGTGA3800.015.6315087
Produced by FastQC (version 0.11.5)