##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062550_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1229396
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25530097706516	32.0	32.0	32.0	32.0	32.0
2	31.415023312260654	32.0	32.0	32.0	32.0	32.0
3	31.49847323401085	32.0	32.0	32.0	32.0	32.0
4	31.604489521683817	32.0	32.0	32.0	32.0	32.0
5	31.538557145134686	32.0	32.0	32.0	32.0	32.0
6	35.13340127997814	36.0	36.0	36.0	36.0	36.0
7	35.14943191616046	36.0	36.0	36.0	36.0	36.0
8	35.09298712538515	36.0	36.0	36.0	36.0	36.0
9	35.20771012757484	36.0	36.0	36.0	36.0	36.0
10	35.08994335429756	36.0	36.0	36.0	36.0	36.0
11	35.2175263300027	36.0	36.0	36.0	36.0	36.0
12	35.1327416064474	36.0	36.0	36.0	36.0	36.0
13	35.16179896469486	36.0	36.0	36.0	36.0	36.0
14	35.12838987600415	36.0	36.0	36.0	36.0	36.0
15	35.09484657506613	36.0	36.0	36.0	36.0	36.0
16	35.11233239737237	36.0	36.0	36.0	36.0	36.0
17	35.08590153213448	36.0	36.0	36.0	36.0	36.0
18	35.09361751624375	36.0	36.0	36.0	36.0	36.0
19	35.07371587348584	36.0	36.0	36.0	36.0	36.0
20	35.065313373396364	36.0	36.0	36.0	36.0	36.0
21	35.06122274677972	36.0	36.0	36.0	36.0	36.0
22	35.04327002853434	36.0	36.0	36.0	36.0	36.0
23	35.013318735379	36.0	36.0	36.0	36.0	36.0
24	34.98659667023482	36.0	36.0	36.0	36.0	36.0
25	34.96036346303388	36.0	36.0	36.0	36.0	36.0
26	34.914488903494075	36.0	36.0	36.0	32.0	36.0
27	34.900208720379766	36.0	36.0	36.0	32.0	36.0
28	34.88640031364996	36.0	36.0	36.0	32.0	36.0
29	34.86274642182015	36.0	36.0	36.0	32.0	36.0
30	34.84129686447654	36.0	36.0	36.0	32.0	36.0
31	34.837564950593624	36.0	36.0	36.0	32.0	36.0
32	34.796116141584974	36.0	36.0	36.0	32.0	36.0
33	34.78118523242308	36.0	36.0	36.0	32.0	36.0
34	34.75603873772161	36.0	36.0	36.0	32.0	36.0
35	34.726785348252314	36.0	36.0	36.0	32.0	36.0
36	34.699249875548645	36.0	36.0	36.0	32.0	36.0
37	34.68270109875093	36.0	36.0	36.0	32.0	36.0
38	34.642894559604876	36.0	36.0	36.0	32.0	36.0
39	34.62839719667219	36.0	36.0	36.0	32.0	36.0
40	34.62170447927275	36.0	36.0	36.0	32.0	36.0
41	34.585412674191225	36.0	36.0	36.0	32.0	36.0
42	34.557817822735714	36.0	36.0	36.0	32.0	36.0
43	34.53758186947086	36.0	36.0	36.0	32.0	36.0
44	34.50261266508107	36.0	36.0	36.0	32.0	36.0
45	34.456265515749195	36.0	36.0	36.0	32.0	36.0
46	34.441898298026025	36.0	36.0	36.0	32.0	36.0
47	34.39503056785608	36.0	36.0	36.0	32.0	36.0
48	34.358642780682544	36.0	36.0	36.0	32.0	36.0
49	34.338602045231966	36.0	36.0	36.0	32.0	36.0
50	33.81955773404176	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	9.0
21	33.0
22	106.0
23	286.0
24	839.0
25	1937.0
26	4169.0
27	7667.0
28	12714.0
29	19740.0
30	28318.0
31	39444.0
32	58383.0
33	95158.0
34	207811.0
35	752781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04541475621622	18.10155189862708	11.538930885353448	26.31410245980326
2	16.106274306603424	20.622737111653585	36.90601595806554	26.364972623677446
3	18.687362075570995	24.380280578857594	28.61865303602527	28.31370430954614
4	12.435954270189809	15.83046945855482	35.956303710361595	35.77727256089378
5	14.233493520395383	36.92788979303658	33.436175162437486	15.402441524130547
6	34.08950780468362	35.42203857197472	16.799957702600476	13.68849592074118
7	29.98377250598872	30.622297959565476	20.911505252583588	18.482424281862215
8	28.124786480515635	32.97407832789435	19.573839511434883	19.32729568015513
9	27.61600005205808	13.988332481966752	18.746929386462945	39.64873807951221
10	16.011765126940382	26.844645663398936	31.67506645539761	25.468522754263066
11	37.43114505008964	21.207975298439234	22.25466814598388	19.10621150548725
12	24.717910258370775	23.9246752063615	28.84790580089735	22.509508734370375
13	29.67733748930369	19.625165528438355	25.35667921483395	25.340817767424
14	23.28281530117228	19.952887434154658	25.390354287796608	31.37394297687645
15	25.040751718730174	27.631292114176393	22.53871006575587	24.789246101337568
16	25.328128609496048	25.741827694249857	24.423050018057648	24.506993678196448
17	23.75459168567329	25.959088853388167	25.639094319486965	24.647225141451575
18	24.540994114182897	24.669837871605242	27.149429475937776	23.63973853827408
19	25.38775138360626	25.014478654558825	25.78632108775366	23.811448874081258
20	25.551164962306693	24.407920637451237	25.72295663886982	24.31795776137225
21	26.35131397857159	24.18699914429525	24.861069988840047	24.600616888293114
22	25.49861883396399	24.790140849653	25.267123042534706	24.444117273848295
23	24.023016180303173	24.56612840777097	26.022046598492267	25.388808813433588
24	24.440131576806824	25.122661859970265	25.931270314853798	24.505936248369117
25	24.644215533481482	24.790140849653	25.78851728816427	24.77712632870125
26	24.036112042010874	25.53180586239096	26.3008013691276	24.13128072647056
27	24.923377007896562	25.19570585881197	25.432976843913597	24.447940289377872
28	24.028710033219564	25.01114368356494	26.08175071335843	24.878395569857066
29	24.152836026796898	25.10281471551884	25.988046162505817	24.756303095178446
30	24.147792899928096	25.180332455937716	26.34448135507192	24.32739328906227
31	24.662354522057985	24.953066383817745	25.38734467982652	24.997234414297754
32	24.263866158666534	25.191720161770498	25.49935090076753	25.04506277879544
33	24.03204500421345	24.81486843946133	26.057917871865534	25.095168684459683
34	24.56759254137804	25.0130958617077	26.292992656556553	24.126318940357706
35	25.047421660717944	24.96762637913252	26.002362135552744	23.982589824596793
36	24.003982443411235	25.36888032822622	25.818613367865197	24.80852386049735
37	24.9370422548959	25.031885576331792	25.363999882869308	24.667072285902996
38	24.150802507898188	25.157719725784045	25.887834351177325	24.803643415140442
39	24.718967688198106	24.819260840282546	25.541160049325036	24.920611422194312
40	25.079469918561635	24.86879736065515	26.00187409101705	24.04985862976616
41	24.060188905771614	25.13486297336253	26.420616302639672	24.384331818226183
42	25.006019215940185	25.44428320899043	25.867905865969952	23.68179170909943
43	24.05571516419445	24.665120107760234	26.153249237837116	25.1259154902082
44	23.69220332586083	25.163088215676645	25.96095969077498	25.183748767687547
45	23.86310025410852	25.388646131921693	25.90182496120046	24.84642865276933
46	23.749505854103727	24.752032302093554	26.189895184131366	25.30856665967135
47	24.312507930723708	24.678541332491726	26.28599735154499	24.72295338523958
48	24.354642442305003	25.6111130994407	25.48576699452414	24.548477463730155
49	23.751338055435355	25.387995405874104	25.86652307311883	24.99414346557171
50	23.428577935831903	26.109406570380898	25.521556927141457	24.940458566645734
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	54.0
2	52.0
3	191.5
4	331.0
5	318.5
6	306.0
7	296.5
8	287.0
9	370.5
10	454.0
11	711.5
12	969.0
13	1641.5
14	2314.0
15	2986.5
16	3659.0
17	4280.5
18	4902.0
19	5123.5
20	5345.0
21	6047.0
22	6749.0
23	7198.0
24	7647.0
25	8683.0
26	9719.0
27	11767.0
28	13815.0
29	16037.0
30	18259.0
31	20382.5
32	22506.0
33	26467.5
34	30429.0
35	34912.0
36	39395.0
37	44611.5
38	49828.0
39	52625.5
40	55423.0
41	59690.5
42	63958.0
43	65117.0
44	66276.0
45	72291.5
46	78307.0
47	82883.0
48	87459.0
49	89907.0
50	92355.0
51	88473.0
52	84591.0
53	83107.5
54	81624.0
55	82271.0
56	82918.0
57	80256.0
58	77594.0
59	71028.5
60	64463.0
61	56778.0
62	49093.0
63	42867.5
64	36642.0
65	31611.0
66	26580.0
67	23410.0
68	20240.0
69	18341.0
70	16442.0
71	12932.5
72	9423.0
73	8364.5
74	7306.0
75	5461.0
76	3616.0
77	3248.0
78	2880.0
79	2508.0
80	2136.0
81	1690.0
82	1244.0
83	1080.5
84	917.0
85	721.5
86	526.0
87	386.5
88	247.0
89	159.5
90	72.0
91	48.0
92	24.0
93	15.5
94	7.0
95	6.5
96	6.0
97	4.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0156174251421023
2	0.003985697041474025
3	7.320668035360454E-4
4	8.134075594844949E-5
5	0.0
6	4.8804453569069686E-4
7	8.134075594844949E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.6268151189689897E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1229396.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.406781485107956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.02297472551338	50.36766885728132
2	11.7038844289673	14.37395747313105
3	2.911243225011897	5.3631022970491955
4	1.1626416017275638	2.855763151311217
5	0.5832133647084853	1.7906627822924257
6	0.3462302669141029	1.275653180635495
7	0.217674593084157	0.9356687320665034
8	0.15610499031686	0.7668724023297854
9	0.11885861256818724	0.6568852364638001
>10	0.6318796979997963	7.322204183104167
>50	0.06888539479268167	2.963291487848079
>100	0.07027504429559187	8.594446576508652
>500	0.005333960086946633	2.1998494005876372
>1k	8.000940130419949E-4	0.5339742393907307
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.6268151189689897E-4	0.0
5	0.0	0.0	0.0	1.6268151189689897E-4	8.134075594844949E-5
6	8.134075594844949E-5	0.0	0.0	1.6268151189689897E-4	1.6268151189689897E-4
7	8.134075594844949E-5	0.0	0.0	1.6268151189689897E-4	1.6268151189689897E-4
8	8.134075594844949E-5	0.0	0.0	1.6268151189689897E-4	1.6268151189689897E-4
9	8.134075594844949E-5	0.0	0.0	2.4402226784534846E-4	1.6268151189689897E-4
10	8.134075594844949E-5	0.0	0.0	2.4402226784534846E-4	2.4402226784534846E-4
11	8.134075594844949E-5	0.0	0.0	2.4402226784534846E-4	2.4402226784534846E-4
12	8.134075594844949E-5	0.0	0.0	3.2536302379379794E-4	2.4402226784534846E-4
13	8.134075594844949E-5	0.0	0.0	3.2536302379379794E-4	2.4402226784534846E-4
14	8.134075594844949E-5	0.0	0.0	3.2536302379379794E-4	2.4402226784534846E-4
15	8.134075594844949E-5	0.0	0.0	3.2536302379379794E-4	2.4402226784534846E-4
16	8.134075594844949E-5	0.0	0.0	3.2536302379379794E-4	2.4402226784534846E-4
17	8.134075594844949E-5	0.0	0.0	4.067037797422474E-4	2.4402226784534846E-4
18	8.134075594844949E-5	0.0	0.0	4.880445356906969E-4	2.4402226784534846E-4
19	8.134075594844949E-5	0.0	0.0	5.693852916391464E-4	2.4402226784534846E-4
20	8.134075594844949E-5	0.0	0.0	7.320668035360454E-4	4.067037797422474E-4
21	8.134075594844949E-5	0.0	0.0	8.947483154329443E-4	4.067037797422474E-4
22	1.6268151189689897E-4	0.0	0.0	0.00154547436302054	4.880445356906969E-4
23	1.6268151189689897E-4	0.0	0.0	0.0027655857022472826	4.880445356906969E-4
24	1.6268151189689897E-4	0.0	0.0	0.004555082333113171	4.880445356906969E-4
25	1.6268151189689897E-4	0.0	0.0	0.00618189745208216	4.880445356906969E-4
26	1.6268151189689897E-4	0.0	0.0	0.008296757106741846	6.507260475875959E-4
27	1.6268151189689897E-4	0.0	0.0	0.011225024320886028	6.507260475875959E-4
28	1.6268151189689897E-4	0.0	0.0	0.019928485207370124	6.507260475875959E-4
29	1.6268151189689897E-4	0.0	0.0	0.037091384712492965	6.507260475875959E-4
30	1.6268151189689897E-4	0.0	0.0	0.0698717093597181	7.320668035360454E-4
31	1.6268151189689897E-4	0.0	0.0	0.14096353005866297	7.320668035360454E-4
32	2.4402226784534846E-4	0.0	0.0	0.22409378263797833	7.320668035360454E-4
33	2.4402226784534846E-4	0.0	0.0	0.31080302847902547	7.320668035360454E-4
34	2.4402226784534846E-4	0.0	0.0	0.412967017950278	7.320668035360454E-4
35	2.4402226784534846E-4	0.0	0.0	0.520092793534386	7.320668035360454E-4
36	2.4402226784534846E-4	0.0	0.0	0.67464022983644	7.320668035360454E-4
37	2.4402226784534846E-4	0.0	0.0	0.8893798255403467	7.320668035360454E-4
38	2.4402226784534846E-4	0.0	0.0	1.187493696091414	8.134075594844948E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	275	0.0	20.799902	6
TACCGTC	290	0.0	20.482664	7
ACCGTCG	290	0.0	19.724047	8
CCGTCGT	285	0.0	19.298157	9
TAGGACG	590	0.0	18.643982	4
CGACGGT	370	0.0	18.432346	7
GACGGTA	365	0.0	18.082108	8
CGTCGTA	310	0.0	17.741854	10
TACGACG	365	0.0	17.47937	5
GTGTATA	315	0.0	17.462368	1
GTCCTAA	480	0.0	17.418713	1
ACGGTAT	370	0.0	17.243164	9
ACGACGG	385	0.0	16.571352	6
ACTATAC	80	0.0019884377	16.499924	3
CGCTTAC	80	0.0019884377	16.499924	18
AGGACGT	650	0.0	16.246078	5
TGTAGGA	645	0.0	16.032238	2
TATGGCG	675	0.0	15.970296	16
CTACGAC	405	0.0	15.753015	4
GACGTGA	380	0.0	15.631508	7
>>END_MODULE