FastQCFastQC Report
Thu 2 Feb 2017
SRR4062549_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062549_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394071
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG68120.4886408224545235No Hit
TCCATGTACTCTGCGTTGATACCAC60770.43591753935057825No Hit
GTATCAACGCAGAGTACTTTTTTTT35640.2556541237856608No Hit
GAGTACATGGAAGCAGTGGTATCAA30740.2205052683830307No Hit
CATGTACTCTGCGTTGATACCACTG28380.2035764319033966No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26530.1903059456799546No Hit
TATCAACGCAGAGTACTTTTTTTTT24580.1763181358768671No Hit
GCTTCCATGTACTCTGCGTTGATAC19800.14203006876981158No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19360.13887384501937133No Hit
GCGCAAGACGGACCAGAGCGAAAGC19140.1372957331441512No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19140.1372957331441512No Hit
GAATAACGCCGCCGCATCGCCAGTC18260.1309832856432707No Hit
CCCATGTACTCTGCGTTGATACCAC17970.1289030472622987No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17640.1265358794494685No Hit
GAACTACGACGGTATCTGATCGTCT17450.12517296464814204No Hit
GTACATGGGAAGCAGTGGTATCAAC17410.12488603521628383No Hit
GAATAGGACCGCGGTTCTATTTTGT17380.12467083814239016No Hit
GCGTTGATACCACTGCTTCCATGTA16720.11993650251672977No Hit
GTATCTGATCGTCTTCGAACCTCCG16610.11914744657911971No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16190.11613468754460855No Hit
GATTAAGAGGGACGGCCGGGGGCAT16070.11527389924903395No Hit
GTCCTATTCCATTATTCCTAGCTGC15940.11434137859549479No Hit
CATGGAAGCAGTGGTATCAACGCAG15880.11391098444770748No Hit
CTATTGGAGCTGGAATTACCGCGGC14470.10379672197470574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAT350.002179521116.2750971
CGCCCGA400.00527264614.2524816
GATAATC500.001498257213.3013587
GCCTTAT500.001506797613.2913291
AAAGCGT655.450142E-513.15424410
GCGTTAT1251.03682396E-1012.9115761
CAAGACG3700.012.835464
AAGACGG3750.012.6647755
GCAAGAC3900.012.663413
GCGCAAG3750.012.4052411
CGTTATT1155.347829E-912.3872282
CGCAAGA3700.012.3202712
GTCCTAC1551.8189894E-1212.25007251
CGACCTC701.0900692E-412.21509514
CGCATCG3450.012.11674413
GTTATTC1501.4551915E-1112.0302393
CGGTTCT3250.011.98540912
ATCCCCG1850.011.81159312
CTTACAC1052.7279748E-711.7588813
GTTCCTA658.0665096E-411.6846861
Produced by FastQC (version 0.11.5)