##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062549_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1394071
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15895460130797	32.0	32.0	32.0	32.0	32.0
2	30.86541431533975	32.0	32.0	32.0	32.0	32.0
3	30.853498136034677	32.0	32.0	32.0	32.0	32.0
4	30.915302018333357	32.0	32.0	32.0	32.0	32.0
5	30.751458856830105	32.0	32.0	32.0	32.0	32.0
6	34.37851587186019	36.0	36.0	36.0	32.0	36.0
7	34.24992557767861	36.0	36.0	36.0	32.0	36.0
8	34.2535911011706	36.0	36.0	36.0	32.0	36.0
9	34.39139398208556	36.0	36.0	36.0	32.0	36.0
10	34.09919509121128	36.0	36.0	36.0	32.0	36.0
11	34.38683323876617	36.0	36.0	36.0	32.0	36.0
12	34.17634252487858	36.0	36.0	36.0	32.0	36.0
13	34.29452588856665	36.0	36.0	36.0	32.0	36.0
14	34.211555939403375	36.0	36.0	36.0	32.0	36.0
15	34.16295870152955	36.0	36.0	36.0	32.0	36.0
16	34.14999522979819	36.0	36.0	36.0	32.0	36.0
17	34.07658935592233	36.0	36.0	36.0	32.0	36.0
18	34.09812269245971	36.0	36.0	36.0	32.0	36.0
19	34.08496123942037	36.0	36.0	36.0	32.0	36.0
20	34.07706709342637	36.0	36.0	36.0	32.0	36.0
21	34.03611437294083	36.0	36.0	36.0	32.0	36.0
22	33.983599830998564	36.0	36.0	36.0	32.0	36.0
23	33.94952552631824	36.0	36.0	36.0	32.0	36.0
24	33.92804885834366	36.0	36.0	36.0	32.0	36.0
25	33.46238964873382	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	62.0
5	152.0
6	234.0
7	57.0
8	230.0
9	270.0
10	183.0
11	50.0
12	87.0
13	65.0
14	276.0
15	310.0
16	481.0
17	649.0
18	895.0
19	1239.0
20	1936.0
21	2677.0
22	4077.0
23	5874.0
24	8113.0
25	11385.0
26	15933.0
27	20505.0
28	28055.0
29	36937.0
30	48499.0
31	66436.0
32	95777.0
33	139405.0
34	314126.0
35	589092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.66162777017352	17.38096467894252	11.247485573432733	25.709921977451227
2	16.707627106485663	20.113154111682118	36.39991449239183	26.779304289440386
3	19.118825425770243	23.90023550881885	28.482722060139036	28.49821700527187
4	12.700157335018844	15.17848879463296	35.31894957889745	36.80240429145075
5	14.54876065793987	36.34628383954212	33.52208226114087	15.582873241377133
6	34.575893866355486	34.68957157712752	16.830401323943438	13.904133232573546
7	30.143400538801846	30.582496046561207	20.824985589598956	18.44911782503799
8	28.61164601022698	32.066663507530954	19.764917223464337	19.556773258777728
9	27.07231591441256	14.69037058737787	18.910863291737304	39.326450206472266
10	16.2283536725501	26.230192245817246	31.234056122448976	26.307397959183675
11	37.20527040789211	21.329552750761646	21.827406063583307	19.637770777762935
12	24.47352732026519	24.05282324970011	28.303895301647046	23.16975412838765
13	29.46809351097066	19.0524652565806	25.476927496678275	26.00251373577046
14	23.787676162048253	19.869771878137733	24.646670391847884	31.695881567966122
15	25.46371170446548	26.59272079628858	22.987877569194087	24.95568993005185
16	26.139641907366457	25.683174702122113	23.698016349799236	24.479167040712195
17	23.97047511450338	25.67411013440186	25.71368445547961	24.64173029561515
18	25.335420184756742	24.096525737155844	26.74282381452785	23.825230263559565
19	25.223223103869586	25.925125178043366	25.227389121092475	23.624262596994576
20	25.164255857046413	24.029260094517145	25.888216958501513	24.91826708993493
21	26.466428633507228	24.581987483506833	24.140469731217898	24.811114151768045
22	25.217083328244648	24.696959791720698	24.86233608744972	25.22362079258493
23	23.823698853114465	24.35511932178163	25.514216467282232	26.306965357821678
24	24.155048804717204	25.951438785617675	24.989207581485452	24.90430482817967
25	24.97110715098911	24.555437996915703	24.977068882419573	25.496385969675618
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	267.0
1	267.0
2	641.0
3	1015.0
4	1015.0
5	1015.0
6	2700.5
7	4386.0
8	4386.0
9	4386.0
10	4468.5
11	4551.0
12	4551.0
13	4551.0
14	4540.5
15	4530.0
16	4530.0
17	4530.0
18	7142.5
19	9755.0
20	9755.0
21	9755.0
22	15465.0
23	21175.0
24	21175.0
25	21175.0
26	31301.0
27	41427.0
28	41427.0
29	41427.0
30	49383.0
31	57339.0
32	57339.0
33	57339.0
34	70919.0
35	84499.0
36	84499.0
37	84499.0
38	94126.0
39	103753.0
40	103753.0
41	103753.0
42	120723.5
43	137694.0
44	137694.0
45	137694.0
46	170306.0
47	202918.0
48	202918.0
49	202918.0
50	194856.0
51	186794.0
52	186794.0
53	186794.0
54	169818.0
55	152842.0
56	152842.0
57	152842.0
58	141695.5
59	130549.0
60	130549.0
61	130549.0
62	116953.5
63	103358.0
64	103358.0
65	103358.0
66	85862.0
67	68366.0
68	68366.0
69	68366.0
70	51819.0
71	35272.0
72	35272.0
73	35272.0
74	27653.5
75	20035.0
76	20035.0
77	20035.0
78	16886.0
79	13737.0
80	13737.0
81	13737.0
82	9573.5
83	5410.0
84	5410.0
85	5410.0
86	4045.0
87	2680.0
88	2680.0
89	2680.0
90	1832.5
91	985.0
92	985.0
93	985.0
94	564.5
95	144.0
96	144.0
97	144.0
98	367.0
99	590.0
100	590.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0082492211659234
2	0.003084491392475706
3	0.004877800341589488
4	0.0164984423318468
5	0.036511770203956614
6	0.047199891540674756
7	0.07029771080526027
8	0.09217607998444842
9	0.0997079775707263
10	0.12101248788619805
11	0.12144288203398536
12	0.134928565331321
13	0.1234513880569928
14	0.12517296464814204
15	0.11556082868089215
16	0.11749760234593505
17	0.125818555869823
18	0.13356565052999453
19	0.13299179166627811
20	0.12416871163663831
21	0.1311267503591998
22	0.15020755757777043
23	0.1400932951047687
24	0.13557415655300198
25	0.13327872109813632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1394071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.060941519324544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.61022297943697	37.465320479756706
2	11.949959901146576	11.247527281322647
3	3.4588858985820767	4.883352809855623
4	1.5178779708656907	2.8573106568152506
5	0.8398196689311419	1.9761352163173485
6	0.513547184366339	1.4500808406526833
7	0.35622182761659327	1.1734894218159984
8	0.26076227958148124	0.9817374711863878
9	0.1941461696032211	0.8223031380508249
>10	1.0294219771615745	8.910331583146343
>50	0.11525330332062617	3.8019299924282937
>100	0.128947870885393	12.958747805557405
>500	0.018049633639657583	5.605032411812482
>1k	0.0065774088686887805	4.938875573019824
>5k	3.059259938925014E-4	0.9278253182622179
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6812	0.4886408224545235	No Hit
TCCATGTACTCTGCGTTGATACCAC	6077	0.43591753935057825	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3564	0.2556541237856608	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3074	0.2205052683830307	No Hit
CATGTACTCTGCGTTGATACCACTG	2838	0.2035764319033966	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2653	0.1903059456799546	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2458	0.1763181358768671	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1980	0.14203006876981158	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1936	0.13887384501937133	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1914	0.1372957331441512	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1914	0.1372957331441512	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1826	0.1309832856432707	No Hit
CCCATGTACTCTGCGTTGATACCAC	1797	0.1289030472622987	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1764	0.1265358794494685	No Hit
GAACTACGACGGTATCTGATCGTCT	1745	0.12517296464814204	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1741	0.12488603521628383	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1738	0.12467083814239016	No Hit
GCGTTGATACCACTGCTTCCATGTA	1672	0.11993650251672977	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1661	0.11914744657911971	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1619	0.11613468754460855	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1607	0.11527389924903395	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1594	0.11434137859549479	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1588	0.11391098444770748	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1447	0.10379672197470574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4346471592910262E-4
2	0.0	0.0	0.0	0.0	1.4346471592910262E-4
3	0.0	0.0	0.0	0.0	1.4346471592910262E-4
4	0.0	0.0	0.0	0.0	1.4346471592910262E-4
5	0.0	0.0	0.0	0.0	1.4346471592910262E-4
6	0.0	0.0	0.0	0.0	2.151970738936539E-4
7	0.0	0.0	0.0	0.0	2.151970738936539E-4
8	0.0	0.0	0.0	0.0	2.151970738936539E-4
9	0.0	0.0	0.0	0.0	2.151970738936539E-4
10	0.0	0.0	0.0	2.8692943185820523E-4	2.8692943185820523E-4
11	0.0	0.0	0.0	2.8692943185820523E-4	2.8692943185820523E-4
12	0.0	0.0	0.0	2.8692943185820523E-4	3.5866178982275654E-4
13	0.0	0.0	0.0	3.5866178982275654E-4	4.303941477873078E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCTAAT	35	0.0021795211	16.275097	1
CGCCCGA	40	0.005272646	14.25248	16
GATAATC	50	0.0014982572	13.301358	7
GCCTTAT	50	0.0015067976	13.291329	1
AAAGCGT	65	5.450142E-5	13.154244	10
GCGTTAT	125	1.03682396E-10	12.911576	1
CAAGACG	370	0.0	12.83546	4
AAGACGG	375	0.0	12.664775	5
GCAAGAC	390	0.0	12.66341	3
GCGCAAG	375	0.0	12.405241	1
CGTTATT	115	5.347829E-9	12.387228	2
CGCAAGA	370	0.0	12.320271	2
GTCCTAC	155	1.8189894E-12	12.2500725	1
CGACCTC	70	1.0900692E-4	12.215095	14
CGCATCG	345	0.0	12.116744	13
GTTATTC	150	1.4551915E-11	12.030239	3
CGGTTCT	325	0.0	11.985409	12
ATCCCCG	185	0.0	11.811593	12
CTTACAC	105	2.7279748E-7	11.758881	3
GTTCCTA	65	8.0665096E-4	11.684686	1
>>END_MODULE