FastQCFastQC Report
Thu 2 Feb 2017
SRR4062549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394071
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA31690.22731984238966307No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC28470.20422202312507753No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC19170.13751093021804484No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18610.13349391817202996No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17540.125818555869823No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16840.12079729081230439No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16330.11713894055611228No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA16190.11613468754460855No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC14810.10623562214550047No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG14450.10365325725877664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1102.0190782E-1023.9998264
TGGTATA2550.019.8429944
TATACTG1800.019.5554125
CAAGACG6500.018.615254
TCAATAC1901.8189894E-1218.526183
TTAGACG600.007412973318.3331994
ATTCGAC600.007412973318.33319914
AAGACGG6650.018.1953545
AGCGAAA6300.018.15859817
CGTTAGG1355.527727E-817.92579515
ATACGAA5700.017.75425540
CAGAGCG6350.017.6691614
CGAATGC5900.017.15241643
AGAGCGA6550.017.12964415
ATACGAT902.2158018E-417.1109856
GACGGAC6700.017.0745017
TCGTTAG1551.4397301E-817.03213314
GCGAAAG6600.016.99987418
GAGCGAA6750.016.94802516
ACGATTA1702.4683686E-916.82340632
Produced by FastQC (version 0.11.5)