##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062549_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1394071
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275755682458065	32.0	32.0	32.0	32.0	32.0
2	31.444720534319988	32.0	32.0	32.0	32.0	32.0
3	31.51499672541786	32.0	32.0	32.0	32.0	32.0
4	31.614942854417027	32.0	32.0	32.0	32.0	32.0
5	31.5547407556717	32.0	32.0	32.0	32.0	32.0
6	35.1552460384012	36.0	36.0	36.0	36.0	36.0
7	35.167439821931595	36.0	36.0	36.0	36.0	36.0
8	35.1075676920329	36.0	36.0	36.0	36.0	36.0
9	35.22035534775488	36.0	36.0	36.0	36.0	36.0
10	35.10938467266014	36.0	36.0	36.0	36.0	36.0
11	35.23755389790047	36.0	36.0	36.0	36.0	36.0
12	35.14534625567851	36.0	36.0	36.0	36.0	36.0
13	35.181664348515966	36.0	36.0	36.0	36.0	36.0
14	35.14693441008385	36.0	36.0	36.0	36.0	36.0
15	35.116885725332494	36.0	36.0	36.0	36.0	36.0
16	35.121743440613855	36.0	36.0	36.0	36.0	36.0
17	35.088687735416634	36.0	36.0	36.0	36.0	36.0
18	35.10031268134837	36.0	36.0	36.0	36.0	36.0
19	35.078418531050424	36.0	36.0	36.0	36.0	36.0
20	35.06857039562547	36.0	36.0	36.0	36.0	36.0
21	35.0559741935669	36.0	36.0	36.0	36.0	36.0
22	35.03993985959108	36.0	36.0	36.0	36.0	36.0
23	34.99310580307603	36.0	36.0	36.0	36.0	36.0
24	34.961281025141474	36.0	36.0	36.0	36.0	36.0
25	34.94359541228531	36.0	36.0	36.0	32.0	36.0
26	34.89528438651977	36.0	36.0	36.0	32.0	36.0
27	34.87264637166974	36.0	36.0	36.0	32.0	36.0
28	34.85447082680868	36.0	36.0	36.0	32.0	36.0
29	34.82702530932786	36.0	36.0	36.0	32.0	36.0
30	34.80837991752213	36.0	36.0	36.0	32.0	36.0
31	34.80097211691513	36.0	36.0	36.0	32.0	36.0
32	34.756474383298986	36.0	36.0	36.0	32.0	36.0
33	34.726025431990195	36.0	36.0	36.0	32.0	36.0
34	34.70516279299978	36.0	36.0	36.0	32.0	36.0
35	34.65667171901575	36.0	36.0	36.0	32.0	36.0
36	34.6292907606571	36.0	36.0	36.0	32.0	36.0
37	34.60135315920064	36.0	36.0	36.0	32.0	36.0
38	34.5513470978164	36.0	36.0	36.0	32.0	36.0
39	34.50269032208546	36.0	36.0	36.0	32.0	36.0
40	34.46445123670172	36.0	36.0	36.0	32.0	36.0
41	34.44147894906357	36.0	36.0	36.0	32.0	36.0
42	34.359192609271695	36.0	36.0	36.0	32.0	36.0
43	34.37129529270747	36.0	36.0	36.0	32.0	36.0
44	34.333049751411515	36.0	36.0	36.0	32.0	36.0
45	34.241154862270285	36.0	36.0	36.0	32.0	36.0
46	34.28633692258142	36.0	36.0	36.0	32.0	36.0
47	34.224246110850885	36.0	36.0	36.0	32.0	36.0
48	34.196436910315185	36.0	36.0	36.0	32.0	36.0
49	34.20196890976141	36.0	36.0	36.0	32.0	36.0
50	33.65224511520575	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	8.0
21	25.0
22	115.0
23	357.0
24	1039.0
25	2387.0
26	5024.0
27	9692.0
28	15984.0
29	24198.0
30	34159.0
31	47727.0
32	70069.0
33	107362.0
34	233539.0
35	842385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.123190344967114	18.096657944976585	11.776649163499762	26.003502546556533
2	16.12713474799805	20.519477165629972	36.09030592883731	27.26308215753466
3	18.530896424188036	24.16056399253691	28.723850677983243	28.584688905291806
4	12.367339589847546	15.625719567280482	35.55352791508586	36.45341292778611
5	14.323517238361605	36.62898087687069	33.40195728911942	15.645544595648284
6	34.79517125073615	34.91690141148166	16.541385744013738	13.74654159376845
7	30.206854600662375	30.695065028969115	20.738828940563288	18.359251429805227
8	28.64911471510418	32.22647913915432	19.641969454927334	19.48243669081417
9	27.23383529246358	14.339441821829734	18.841364607684973	39.58535827802171
10	16.252615541102283	26.330437976258025	31.175313165541784	26.24163331709791
11	37.556910659500126	21.160256543605023	21.71324129115375	19.569591505741098
12	24.477447705317733	24.087510607422434	28.252291310844285	23.182750376415548
13	29.64031243745835	19.004842651486186	25.46950621596748	25.885338695087984
14	23.888955440576556	19.670088539249438	24.72937174648924	31.711584273684768
15	25.577893808851915	26.56206176012556	22.8995510271715	24.96049340385102
16	26.29241982653681	25.518427684099304	23.728059761662067	24.461092727701818
17	24.00659650763842	25.670141621194333	25.646469943066023	24.676791928101224
18	25.56204095774175	23.839818775370837	26.784145140383814	23.813995126503603
19	25.326328429470234	25.778887875868588	25.274465934661865	23.620317759999313
20	25.26356261625125	23.908036247795124	25.98540533444853	24.84299580150509
21	26.736658319411276	24.39696399968151	24.16354690686486	24.702830774042354
22	25.297492021568484	24.574501585643773	24.91881690387362	25.209189488914124
23	23.880132360546916	24.050783640144584	25.688863766623076	26.380220232685424
24	24.29625176909928	25.783980873284072	25.03896860346424	24.880798754152405
25	25.138461383961076	24.21261183971261	25.09240921014783	25.55651756617848
26	24.229684140908176	25.039542462327958	25.860160637442426	24.87061275932144
27	25.59102083035943	24.914010835889993	24.894427902165674	24.600540431584903
28	24.454421618411114	23.95918141902385	26.057926748350695	25.528470214214337
29	24.137436328565762	25.09255267486376	26.166242608877166	24.603768387693307
30	24.89772759063204	24.666534200912295	26.152039602000187	24.28369860645548
31	24.583539862747305	24.756343113083908	24.97648971967712	25.68362730449166
32	24.360308764761623	24.871401815259052	25.096139292761986	25.672150127217336
33	24.112616932710026	24.392229664055847	25.62667181226781	25.868481590966315
34	24.702472112252533	24.721768116544997	26.137693130407275	24.4380666407952
35	25.969911145128187	24.42737851945848	25.501570579977635	24.1011397554357
36	24.519052472937176	25.241253852924277	25.177698983767684	25.061994690370863
37	25.864823240710123	24.842565407357302	25.019672599171777	24.2729387527608
38	24.501406312877897	24.58059883607076	25.500351129892234	25.41764372115911
39	24.818248138007316	24.542508953991582	25.129136177425682	25.510106730575416
40	25.372739265073303	24.841632886703763	25.411618203090086	24.37400964513285
41	23.82583096556775	25.064863984689445	26.111080425602427	24.998224624140377
42	25.90212406685169	25.333788594698547	25.178846701495118	23.585240636954648
43	24.817172152637852	23.881064881200455	25.37568029174985	25.926082674411848
44	24.36640601518861	24.571058432461474	25.80571577774733	25.256819774602583
45	24.555779440215026	24.796584965902024	25.584134523994834	25.063501069888115
46	24.191844162663397	24.503378240244924	26.032642573645926	25.272135023445756
47	24.65914576804194	24.207949236444914	26.344712715492967	24.788192280020173
48	25.291179574067606	25.90054595497647	24.548893133850427	24.2593813371055
49	23.797353219455825	25.08071683579961	26.001186453200738	25.120743491543827
50	23.622613195454175	26.09192788602589	24.88417017497674	25.401288743543194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	20.0
2	24.0
3	99.5
4	175.0
5	229.0
6	283.0
7	264.5
8	246.0
9	341.5
10	437.0
11	679.0
12	921.0
13	1525.5
14	2130.0
15	3001.5
16	3873.0
17	4758.5
18	5644.0
19	5967.0
20	6290.0
21	7162.0
22	8034.0
23	8145.5
24	8257.0
25	9193.0
26	10129.0
27	12399.5
28	14670.0
29	16388.5
30	18107.0
31	19147.5
32	20188.0
33	23070.0
34	25952.0
35	29989.5
36	34027.0
37	42321.0
38	50615.0
39	54499.5
40	58384.0
41	64777.0
42	71170.0
43	72596.0
44	74022.0
45	84548.5
46	95075.0
47	103276.0
48	111477.0
49	111109.0
50	110741.0
51	103087.0
52	95433.0
53	93435.0
54	91437.0
55	94054.0
56	96671.0
57	94441.0
58	92211.0
59	84551.0
60	76891.0
61	67878.0
62	58865.0
63	51518.0
64	44171.0
65	37677.0
66	31183.0
67	27123.0
68	23063.0
69	21414.5
70	19766.0
71	15300.5
72	10835.0
73	9992.0
74	9149.0
75	6502.5
76	3856.0
77	3639.0
78	3422.0
79	2958.0
80	2494.0
81	1962.0
82	1430.0
83	1269.0
84	1108.0
85	926.0
86	744.0
87	534.0
88	324.0
89	199.0
90	74.0
91	50.5
92	27.0
93	20.0
94	13.0
95	10.5
96	8.0
97	5.5
98	3.0
99	4.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0163549776159177
2	0.00387354733008577
3	7.173235796455131E-4
4	2.869294318582052E-4
5	0.0
6	2.869294318582052E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.434647159291026E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1394071.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.33657242302362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60280756828033	41.3787186822806
2	11.540946076355322	11.849452281580506
3	3.2451519691671025	4.997849372665939
4	1.4016066041252278	2.878147157650518
5	0.7649428990493699	1.9634773268262813
6	0.4857471144244057	1.4961955151353947
7	0.3556886609066016	1.278188569047603
8	0.2551072756850112	1.0477066507075063
9	0.17345405255568358	0.8014082877982616
>10	0.9425238565989839	8.853169545510275
>50	0.10544647987861958	3.8307883375923377
>100	0.10970046830585858	12.204130606519156
>500	0.013220296822944892	4.6246587989318995
>1k	0.0036566778446443316	2.7961088677538237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3169	0.22731984238966307	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2847	0.20422202312507753	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1917	0.13751093021804484	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1861	0.13349391817202996	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1754	0.125818555869823	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1684	0.12079729081230439	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1633	0.11713894055611228	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1619	0.11613468754460855	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1481	0.10623562214550047	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1445	0.10365325725877664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.173235796455131E-5
2	0.0	0.0	0.0	0.0	7.173235796455131E-5
3	0.0	0.0	0.0	0.0	7.173235796455131E-5
4	0.0	0.0	0.0	0.0	7.173235796455131E-5
5	0.0	0.0	0.0	0.0	7.173235796455131E-5
6	0.0	0.0	0.0	0.0	2.151970738936539E-4
7	0.0	0.0	0.0	0.0	2.151970738936539E-4
8	0.0	0.0	0.0	0.0	2.151970738936539E-4
9	0.0	0.0	0.0	0.0	2.151970738936539E-4
10	0.0	0.0	0.0	1.4346471592910262E-4	2.151970738936539E-4
11	0.0	0.0	0.0	1.4346471592910262E-4	2.151970738936539E-4
12	0.0	0.0	0.0	1.4346471592910262E-4	5.021265057518591E-4
13	0.0	0.0	0.0	2.151970738936539E-4	5.021265057518591E-4
14	0.0	0.0	0.0	2.8692943185820523E-4	5.021265057518591E-4
15	0.0	0.0	0.0	3.5866178982275654E-4	6.455912216809617E-4
16	0.0	0.0	0.0	3.5866178982275654E-4	6.455912216809617E-4
17	0.0	0.0	0.0	3.5866178982275654E-4	6.455912216809617E-4
18	0.0	0.0	0.0	3.5866178982275654E-4	7.173235796455131E-4
19	0.0	0.0	0.0	4.303941477873078E-4	7.173235796455131E-4
20	0.0	0.0	0.0	4.303941477873078E-4	0.0010042530115037182
21	0.0	0.0	0.0	7.173235796455131E-4	0.0010759853694682696
22	0.0	0.0	0.0	0.001147717727432821	0.0012911824433619234
23	0.0	0.0	0.0	0.0020802383809719878	0.0014346471592910262
24	0.0	0.0	0.0	0.003730082614156668	0.0014346471592910262
25	0.0	0.0	0.0	0.004877800341589488	0.0014346471592910262
26	0.0	0.0	0.0	0.006168982784951412	0.0015063795172555773
27	0.0	0.0	0.0	0.009612135967249875	0.0015063795172555773
28	0.0	0.0	0.0	0.018793877786712442	0.0015063795172555773
29	0.0	0.0	0.0	0.03357074352741001	0.0015063795172555773
30	0.0	0.0	0.0	0.06283754557694694	0.0015063795172555773
31	0.0	0.0	0.0	0.13069635621141248	0.00164984423318468
32	0.0	0.0	0.0	0.2122560472171073	0.0017215765911492312
33	0.0	0.0	0.0	0.2930984146431566	0.0017215765911492312
34	0.0	0.0	0.0	0.3857048887753924	0.0017215765911492312
35	0.0	0.0	0.0	0.4840499515447922	0.0017215765911492312
36	0.0	0.0	0.0	0.6334684531849526	0.0017215765911492312
37	0.0	0.0	0.0	0.8488089917945356	0.0017215765911492312
38	0.0	0.0	0.0	1.1147208427691273	0.0017215765911492312
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	110	2.0190782E-10	23.999826	4
TGGTATA	255	0.0	19.84299	44
TATACTG	180	0.0	19.555412	5
CAAGACG	650	0.0	18.61525	4
TCAATAC	190	1.8189894E-12	18.52618	3
TTAGACG	60	0.0074129733	18.333199	4
ATTCGAC	60	0.0074129733	18.333199	14
AAGACGG	665	0.0	18.195354	5
AGCGAAA	630	0.0	18.158598	17
CGTTAGG	135	5.527727E-8	17.925795	15
ATACGAA	570	0.0	17.754255	40
CAGAGCG	635	0.0	17.66916	14
CGAATGC	590	0.0	17.152416	43
AGAGCGA	655	0.0	17.129644	15
ATACGAT	90	2.2158018E-4	17.110985	6
GACGGAC	670	0.0	17.074501	7
TCGTTAG	155	1.4397301E-8	17.032133	14
GCGAAAG	660	0.0	16.999874	18
GAGCGAA	675	0.0	16.948025	16
ACGATTA	170	2.4683686E-9	16.823406	32
>>END_MODULE