FastQCFastQC Report
Thu 2 Feb 2017
SRR4062548_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062548_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259779
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG71040.5639084315582337No Hit
TCCATGTACTCTGCGTTGATACCAC57310.4549210615512721No Hit
GTATCAACGCAGAGTACTTTTTTTT35270.27996974072436515No Hit
GAGTACATGGAAGCAGTGGTATCAA29780.23639066852201857No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28490.22615077723949994No Hit
TATCAACGCAGAGTACTTTTTTTTT27270.2164665389723118No Hit
CATGTACTCTGCGTTGATACCACTG22750.18058722998240168No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19900.15796421435823269No Hit
GCTTCCATGTACTCTGCGTTGATAC18730.14867687110199487No Hit
CCCATGTACTCTGCGTTGATACCAC17860.14177089791145908No Hit
GTACATGGGAAGCAGTGGTATCAAC16510.13105473261580008No Hit
CATGGAAGCAGTGGTATCAACGCAG16010.12708578250629674No Hit
GCGTTGATACCACTGCTTCCATGTA15930.12645075048877621No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15340.12176738935956227No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13580.10779668497411055No Hit
GAATAGGACCGCGGTTCTATTTTGT13270.10533593590621848No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13270.10533593590621848No Hit
GTATCAACGCAGAGTACATGGAAGC13060.10366897686022708No Hit
GTACATGGGGTGGTATCAACGCAAA12730.10104946978795487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA453.537007E-516.8894549
TCGCGTA951.8189894E-1116.0005369
GTCGCGT1004.0017767E-1115.2005098
CGAACGA1200.015.04456216
ACGAACG1250.014.4427815
GAACTCG400.00527719314.2504769
GGTTCTA2600.013.52129613
ATAACGA1205.0931703E-1113.46038912
GGTCGCG1205.0931703E-1113.4587837
CGGCAAA500.001497097313.30256114
TAGGTTG500.001500239513.298865
CGCCAGT1950.013.15637818
CGCGTAA1102.743036E-912.95497910
GCGTAAC1102.746674E-912.95343511
CTAAACG604.0857962E-412.66860212
TAGAGTG1800.012.665585
GTATTAC604.1119015E-412.659041
GATAACG1301.9826984E-1012.42201211
CGCATCG2100.012.2161513
AATCGTA701.0890286E-412.2161513
Produced by FastQC (version 0.11.5)