##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062548_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1259779
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.029486917943544	32.0	32.0	32.0	32.0	32.0
2	30.665725496297366	32.0	32.0	32.0	32.0	32.0
3	30.668326746199135	32.0	32.0	32.0	32.0	32.0
4	30.724985096592338	32.0	32.0	32.0	32.0	32.0
5	30.529965176431737	32.0	32.0	32.0	32.0	32.0
6	34.16096632822106	36.0	36.0	36.0	32.0	36.0
7	34.00537475223829	36.0	36.0	36.0	32.0	36.0
8	33.9853061529046	36.0	36.0	36.0	32.0	36.0
9	34.14434357137244	36.0	36.0	36.0	32.0	36.0
10	33.796955656508004	36.0	36.0	36.0	32.0	36.0
11	34.18255821060678	36.0	36.0	36.0	32.0	36.0
12	33.9151176515881	36.0	36.0	36.0	32.0	36.0
13	34.04857518660019	36.0	36.0	36.0	32.0	36.0
14	33.95376093743426	36.0	36.0	36.0	32.0	36.0
15	33.906183544891604	36.0	36.0	36.0	32.0	36.0
16	33.89431241511408	36.0	36.0	36.0	32.0	36.0
17	33.80408627227474	36.0	36.0	36.0	32.0	36.0
18	33.82811270865763	36.0	36.0	36.0	32.0	36.0
19	33.80650495047147	36.0	36.0	36.0	32.0	36.0
20	33.78506865093004	36.0	36.0	36.0	32.0	36.0
21	33.74268978924081	36.0	36.0	36.0	32.0	36.0
22	33.70898308354084	36.0	36.0	36.0	27.0	36.0
23	33.64849866524208	36.0	36.0	36.0	27.0	36.0
24	33.641757006586076	36.0	36.0	36.0	27.0	36.0
25	33.155061324248145	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	7.0
4	45.0
5	139.0
6	211.0
7	50.0
8	196.0
9	243.0
10	134.0
11	42.0
12	108.0
13	70.0
14	277.0
15	371.0
16	563.0
17	711.0
18	1059.0
19	1601.0
20	2275.0
21	3449.0
22	5104.0
23	7395.0
24	10018.0
25	13368.0
26	18146.0
27	22594.0
28	29600.0
29	38311.0
30	48546.0
31	64885.0
32	91410.0
33	129065.0
34	276680.0
35	493103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.16145506276709	17.29192946968897	11.17169197646661	25.374923491077332
2	16.243845825322257	20.55164902509617	37.04253505160695	26.161970097974617
3	18.92871941993735	24.955506197379428	28.439994093953764	27.675780288729452
4	12.27496752927129	15.683123795248635	35.84045995700208	36.20144871847799
5	14.122985127723389	37.268675312052586	33.526029461861654	15.082310098362376
6	33.770944086100066	35.26722819345196	17.388852798553987	13.572974921893985
7	29.608426437470957	31.01299155211515	21.19573117694486	18.182850833469033
8	28.35310867779298	32.44888522355117	20.131046416841063	19.066959681814787
9	27.450114141735988	14.397194171262662	18.662590989596534	39.49010069740482
10	15.627649995191762	27.12991066213023	31.965011917279217	25.277427425398784
11	37.05688850952089	21.343423674311516	22.08350917516867	19.516178640998923
12	24.02297114696765	24.28256895318337	29.15785708608219	22.53660281376679
13	29.734040777658915	19.721070258567934	25.526013361542095	25.018875602231056
14	23.710833836036805	19.809616459107755	25.451817163766883	31.02773254108856
15	25.233384909669887	27.06223888982929	23.41732567555862	24.287050524942206
16	25.534053802329566	25.97947942817045	24.47901890337085	24.007447866129137
17	23.612011851625784	25.936637673419042	26.146695659914293	24.304654815040884
18	24.72906928593513	24.714524270702604	27.292966014737356	23.263440428624907
19	24.884964418256576	26.19949780977339	25.60001716889664	23.31552060307339
20	24.949849947227204	24.29273324940551	26.480261066391993	24.277155736975292
21	26.303979957540125	24.873999407056033	24.302833811159267	24.519186824244574
22	24.821977270649402	25.187523104993065	25.190623645997718	24.799875978359815
23	23.592977067439964	24.97486075041753	25.557852865635976	25.874309316506533
24	23.84054267380036	26.390883792357638	25.32281667033632	24.44575686350568
25	24.658578420852667	24.977068996488416	25.254784512153826	25.109568070505084
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	317.0
1	317.0
2	692.0
3	1067.0
4	1067.0
5	1067.0
6	2748.5
7	4430.0
8	4430.0
9	4430.0
10	4501.5
11	4573.0
12	4573.0
13	4573.0
14	4540.0
15	4507.0
16	4507.0
17	4507.0
18	7051.5
19	9596.0
20	9596.0
21	9596.0
22	14939.0
23	20282.0
24	20282.0
25	20282.0
26	29957.0
27	39632.0
28	39632.0
29	39632.0
30	48647.0
31	57662.0
32	57662.0
33	57662.0
34	69279.5
35	80897.0
36	80897.0
37	80897.0
38	91007.5
39	101118.0
40	101118.0
41	101118.0
42	116487.0
43	131856.0
44	131856.0
45	131856.0
46	158776.0
47	185696.0
48	185696.0
49	185696.0
50	176485.5
51	167275.0
52	167275.0
53	167275.0
54	150667.0
55	134059.0
56	134059.0
57	134059.0
58	122498.0
59	110937.0
60	110937.0
61	110937.0
62	98630.0
63	86323.0
64	86323.0
65	86323.0
66	71281.5
67	56240.0
68	56240.0
69	56240.0
70	42540.5
71	28841.0
72	28841.0
73	28841.0
74	22619.5
75	16398.0
76	16398.0
77	16398.0
78	13504.5
79	10611.0
80	10611.0
81	10611.0
82	7360.5
83	4110.0
84	4110.0
85	4110.0
86	3032.0
87	1954.0
88	1954.0
89	1954.0
90	1345.0
91	736.0
92	736.0
93	736.0
94	435.5
95	135.0
96	135.0
97	135.0
98	331.0
99	527.0
100	527.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009684238267188134
2	0.005159635142354333
3	0.004207087116073534
4	0.014526357400782201
5	0.036196824998670406
6	0.04707174829870953
7	0.07017103793601893
8	0.09057144149886606
9	0.09970002675072373
10	0.12081484133328146
11	0.12271993738584308
12	0.13327734467712193
13	0.12232304237489273
14	0.12557758146468548
15	0.11509955317559667
16	0.11367073113617548
17	0.12343434840555367
18	0.12827646753914773
19	0.13415051370121267
20	0.12343434840555367
21	0.13097535361361
22	0.15351899023558893
23	0.14177089791145908
24	0.13589685174939414
25	0.1315310066289405
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1259779.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.86453535266883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91673377195988	40.92979728632968
2	12.842592585807678	13.3215019437309
3	3.710985856424444	5.774056714313387
4	1.5096181203697188	3.13182569491779
5	0.8137771183196592	2.1103086061141463
6	0.4472333096380174	1.3917328679167225
7	0.30451701712097695	1.1055543519972117
8	0.20947184444553538	0.8691327905307373
9	0.16119719386851178	0.7524375804129996
>10	0.8806137745287222	8.36623742078855
>50	0.09176462412576879	3.377051027430944
>100	0.09677195617179539	10.629052034965504
>500	0.00996858092973542	3.501231119808886
>1k	0.004447520722497341	3.719173172339132
>5k	3.0672556706878216E-4	1.020907388403541
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7104	0.5639084315582337	No Hit
TCCATGTACTCTGCGTTGATACCAC	5731	0.4549210615512721	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3527	0.27996974072436515	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2978	0.23639066852201857	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2849	0.22615077723949994	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2727	0.2164665389723118	No Hit
CATGTACTCTGCGTTGATACCACTG	2275	0.18058722998240168	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1990	0.15796421435823269	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1873	0.14867687110199487	No Hit
CCCATGTACTCTGCGTTGATACCAC	1786	0.14177089791145908	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1651	0.13105473261580008	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1601	0.12708578250629674	No Hit
GCGTTGATACCACTGCTTCCATGTA	1593	0.12645075048877621	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1534	0.12176738935956227	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1358	0.10779668497411055	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1327	0.10533593590621848	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1327	0.10533593590621848	No Hit
GTATCAACGCAGAGTACATGGAAGC	1306	0.10366897686022708	No Hit
GTACATGGGGTGGTATCAACGCAAA	1273	0.10104946978795487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	7.937900219006666E-5
6	0.0	0.0	0.0	0.0	7.937900219006666E-5
7	0.0	0.0	0.0	7.937900219006666E-5	7.937900219006666E-5
8	0.0	0.0	0.0	7.937900219006666E-5	7.937900219006666E-5
9	0.0	0.0	0.0	7.937900219006666E-5	1.5875800438013333E-4
10	0.0	0.0	0.0	7.937900219006666E-5	1.5875800438013333E-4
11	0.0	0.0	0.0	7.937900219006666E-5	1.5875800438013333E-4
12	0.0	0.0	0.0	7.937900219006666E-5	3.1751600876026666E-4
13	0.0	0.0	0.0	7.937900219006666E-5	3.1751600876026666E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCTAA	45	3.537007E-5	16.889454	9
TCGCGTA	95	1.8189894E-11	16.000536	9
GTCGCGT	100	4.0017767E-11	15.200509	8
CGAACGA	120	0.0	15.044562	16
ACGAACG	125	0.0	14.44278	15
GAACTCG	40	0.005277193	14.250476	9
GGTTCTA	260	0.0	13.521296	13
ATAACGA	120	5.0931703E-11	13.460389	12
GGTCGCG	120	5.0931703E-11	13.458783	7
CGGCAAA	50	0.0014970973	13.302561	14
TAGGTTG	50	0.0015002395	13.29886	5
CGCCAGT	195	0.0	13.156378	18
CGCGTAA	110	2.743036E-9	12.954979	10
GCGTAAC	110	2.746674E-9	12.953435	11
CTAAACG	60	4.0857962E-4	12.668602	12
TAGAGTG	180	0.0	12.66558	5
GTATTAC	60	4.1119015E-4	12.65904	1
GATAACG	130	1.9826984E-10	12.422012	11
CGCATCG	210	0.0	12.21615	13
AATCGTA	70	1.0890286E-4	12.21615	13
>>END_MODULE