FastQCFastQC Report
Thu 2 Feb 2017
SRR4062548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259779
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32860.2608394011965591No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27300.21670467597888202No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13700.10874923300039133No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13370.10612972592811915No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12860.10208139681642572No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12840.1019226388120456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCC450.001398290624.4443114
ATACCGA551.5937284E-423.9998726
ATACCGT3700.021.4052896
TACCGTC3550.021.0703077
ACCGTCG3650.020.493048
CGTCGTA3550.020.45059210
CCGTCGT3700.019.6215159
CTAATAG708.123879E-418.857043
GGCGTTA3050.018.0326942
GTAAACG3200.017.87490327
TACACCG1002.4832636E-517.5999055
GTCGTAG4150.017.49388111
CGCAATA4450.017.30327836
TAGCGGC4350.017.19530930
GATATAC2850.016.986411
CAATACG4550.016.92298338
AATACGA4550.016.92298339
ATACGAA4700.016.85097340
ACCGTGT1702.4683686E-916.8234398
CTAGCGG4500.016.62213329
Produced by FastQC (version 0.11.5)