FastQCFastQC Report
Thu 2 Feb 2017
SRR4062547_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062547_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3632682
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45440.12508664397269015No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG44640.12288441432528362No Hit
GTCTTGCGCCGGTCCAAGAATTTCA44490.12247149626639492No Hit
GATTAAGAGGGACGGCCGGGGGCAT42970.11828725993632253No Hit
GCGCAAGACGGACCAGAGCGAAAGC41680.11473616462987953No Hit
GAATAGGACCGCGGTTCTATTTTGT41070.11305696452373205No Hit
GAATAACGCCGCCGCATCGCCAGTC40880.11253393498247301No Hit
GTATCTGATCGTCTTCGAACCTCCG39810.10958845282906678No Hit
GTCCTATTCCATTATTCCTAGCTGC39540.10884520032306709No Hit
GAACTACGACGGTATCTGATCGTCT37120.10218345563966237No Hit
CTATTGGAGCTGGAATTACCGCGGC36560.10064189488647782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG5850.013.317698513
CGTTATT3700.012.83340552
ACGCTTA1800.012.66892817
GCGTTAT3850.012.5788571
CGCTTAC2050.012.51460218
CTCGACG1750.012.487082513
ATCGCCA6550.012.32959616
CATCGCC6750.012.10502915
GCATCGC6650.011.85860514
CGCCAGT6950.011.75765618
GTATCAA50900.011.7158451
CGAACGA3250.011.69358816
CCGTCGT6950.011.6196589
TCGCCAG6900.011.56728217
CGTCGTA6950.011.48295610
CGACGCT2000.011.4012515
AATCGTA1751.8189894E-1211.40124913
CGGTCCA7500.011.40093410
ACGGTAT6650.011.143779
GTTATTC4950.011.1279313
Produced by FastQC (version 0.11.5)