##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062547_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3632682
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21764553021707	32.0	32.0	32.0	32.0	32.0
2	30.793471875600453	32.0	32.0	32.0	32.0	32.0
3	30.77089131391077	32.0	32.0	32.0	32.0	32.0
4	30.870579092802508	32.0	32.0	32.0	32.0	32.0
5	30.609212972674182	32.0	32.0	32.0	32.0	32.0
6	34.287777735568376	36.0	36.0	36.0	32.0	36.0
7	34.16401325522025	36.0	36.0	36.0	32.0	36.0
8	34.167562698854454	36.0	36.0	36.0	32.0	36.0
9	34.36228026565496	36.0	36.0	36.0	32.0	36.0
10	33.96462778740336	36.0	36.0	36.0	32.0	36.0
11	34.34033091803797	36.0	36.0	36.0	32.0	36.0
12	34.0690817418095	36.0	36.0	36.0	32.0	36.0
13	34.22033968291196	36.0	36.0	36.0	32.0	36.0
14	34.11260605800342	36.0	36.0	36.0	32.0	36.0
15	34.02401421319014	36.0	36.0	36.0	32.0	36.0
16	34.0340508748082	36.0	36.0	36.0	32.0	36.0
17	33.952222077242105	36.0	36.0	36.0	32.0	36.0
18	33.965289282133696	36.0	36.0	36.0	32.0	36.0
19	33.9505170009376	36.0	36.0	36.0	32.0	36.0
20	33.96245831592196	36.0	36.0	36.0	32.0	36.0
21	33.943024189841005	36.0	36.0	36.0	32.0	36.0
22	33.885393216361905	36.0	36.0	36.0	32.0	36.0
23	33.84352332519059	36.0	36.0	36.0	32.0	36.0
24	33.81053888008915	36.0	36.0	36.0	32.0	36.0
25	33.351205528036864	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	23.0
4	124.0
5	315.0
6	617.0
7	140.0
8	558.0
9	631.0
10	446.0
11	145.0
12	269.0
13	205.0
14	449.0
15	519.0
16	956.0
17	1252.0
18	1800.0
19	2564.0
20	4079.0
21	6276.0
22	9946.0
23	15074.0
24	22052.0
25	32026.0
26	45111.0
27	58457.0
28	80215.0
29	105594.0
30	136814.0
31	187517.0
32	269552.0
33	388032.0
34	842502.0
35	1418422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28465768343668	17.24428325310167	11.146025297888611	26.325033765573046
2	17.404945509322932	19.633794240296343	36.574189797354364	26.38707045302636
3	19.09063254898452	23.26703876779926	28.14331628825679	29.49901239495943
4	12.945573122007303	15.308757064635634	35.574652770130086	36.17101704322698
5	15.183273898855829	35.973013534538545	33.09639400239573	15.74731856420989
6	34.37432612523144	35.11325777949262	16.742608824492507	13.769807270783435
7	30.71086922029327	30.31326991136791	20.35403251753029	18.62182835080853
8	28.366979989442026	32.06059043103648	19.36063087755725	20.211798701964245
9	27.65947949490307	14.307414987804624	18.419676384393004	39.613429132899306
10	16.358682561126923	25.81482810490697	30.864031149526216	26.962458184439896
11	37.665303440659706	21.07119854106614	21.995847760020045	19.26765025825411
12	25.096853845307837	23.657835176324305	27.94065639825254	23.304654580115322
13	29.356790550819987	19.076099898135123	25.079430350441633	26.487679200603253
14	23.84972457553543	19.722298935730983	24.39643924372791	32.03153724500567
15	25.6465833216668	26.509723277109813	21.830744097191985	26.0129493040314
16	26.442416866742068	25.525294380912694	22.892539116669862	25.139749635675372
17	24.678960248205144	25.340429056230086	24.556809008323206	25.42380168724156
18	25.533406247622658	24.404190293064726	25.433874935088273	24.62852852422434
19	26.098658021139347	24.728894690795787	24.817898711274704	24.354548576790155
20	26.130004542067038	23.982720301275748	24.295869364860277	25.59140579179694
21	27.450293477616732	23.75538340897095	23.80089001101144	24.993433102400882
22	26.41955642454373	23.98277793354501	24.213644272300975	25.38402136961028
23	24.913448356945345	23.805030484317054	24.896880943146204	26.3846402155914
24	25.089846207109083	24.828618256572874	24.44839473122767	25.633140805090378
25	25.185901835451585	24.19248674886094	24.726639268575713	25.894972147111762
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	262.0
1	262.0
2	718.5
3	1175.0
4	1175.0
5	1175.0
6	3004.0
7	4833.0
8	4833.0
9	4833.0
10	5526.0
11	6219.0
12	6219.0
13	6219.0
14	8373.5
15	10528.0
16	10528.0
17	10528.0
18	17291.5
19	24055.0
20	24055.0
21	24055.0
22	39092.5
23	54130.0
24	54130.0
25	54130.0
26	79452.0
27	104774.0
28	104774.0
29	104774.0
30	126213.0
31	147652.0
32	147652.0
33	147652.0
34	185804.5
35	223957.0
36	223957.0
37	223957.0
38	251625.0
39	279293.0
40	279293.0
41	279293.0
42	317544.5
43	355796.0
44	355796.0
45	355796.0
46	403802.5
47	451809.0
48	451809.0
49	451809.0
50	460651.5
51	469494.0
52	469494.0
53	469494.0
54	438923.0
55	408352.0
56	408352.0
57	408352.0
58	379426.5
59	350501.0
60	350501.0
61	350501.0
62	317343.0
63	284185.0
64	284185.0
65	284185.0
66	242567.0
67	200949.0
68	200949.0
69	200949.0
70	155328.0
71	109707.0
72	109707.0
73	109707.0
74	88678.0
75	67649.0
76	67649.0
77	67649.0
78	55338.5
79	43028.0
80	43028.0
81	43028.0
82	31201.0
83	19374.0
84	19374.0
85	19374.0
86	14360.0
87	9346.0
88	9346.0
89	9346.0
90	6375.5
91	3405.0
92	3405.0
93	3405.0
94	2055.0
95	705.0
96	705.0
97	705.0
98	1104.5
99	1504.0
100	1504.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007817915248293134
2	0.0036887346594059152
3	0.004321875683035289
4	0.013929102519846217
5	0.033914336570060356
6	0.0448429011953152
7	0.06590172219864
8	0.08803413015507551
9	0.09464081909729506
10	0.11693839427728604
11	0.11779175826565608
12	0.13194108375024294
13	0.11963612559535904
14	0.12288441432528362
15	0.11203843331180653
16	0.11148787589995492
17	0.12092993551321035
18	0.12877537863209607
19	0.13092255253831742
20	0.12051701745432163
21	0.12764673593780024
22	0.1507150914943835
23	0.13981405473972125
24	0.13257422477387232
25	0.1291607688203922
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3632682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.348821922343056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.01598352823623	36.62297749569014
2	12.45414661451279	11.5447004726161
3	3.5980633097799775	5.002979868309253
4	1.515360246409213	2.8094064883607404
5	0.774738534987718	1.7954109197261345
6	0.4815317519432188	1.339105765246207
7	0.35326583122561706	1.1461418571907234
8	0.2501090167141315	0.9273806621484483
9	0.19205246456591624	0.8011264931921477
>10	1.1331284807767412	9.73828272623531
>50	0.0995218248957242	3.231207797394858
>100	0.10342470158011745	10.181712657055707
>500	0.016870385671402227	5.411677341999846
>1k	0.01180330870124115	9.447889454834394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4544	0.12508664397269015	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	4464	0.12288441432528362	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	4449	0.12247149626639492	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	4297	0.11828725993632253	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	4168	0.11473616462987953	No Hit
GAATAGGACCGCGGTTCTATTTTGT	4107	0.11305696452373205	No Hit
GAATAACGCCGCCGCATCGCCAGTC	4088	0.11253393498247301	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3981	0.10958845282906678	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3954	0.10884520032306709	No Hit
GAACTACGACGGTATCTGATCGTCT	3712	0.10218345563966237	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3656	0.10064189488647782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.752787059258146E-5
2	0.0	0.0	0.0	0.0	2.752787059258146E-5
3	0.0	0.0	0.0	0.0	2.752787059258146E-5
4	0.0	2.752787059258146E-5	0.0	0.0	2.752787059258146E-5
5	0.0	2.752787059258146E-5	0.0	0.0	5.505574118516292E-5
6	0.0	2.752787059258146E-5	0.0	0.0	5.505574118516292E-5
7	0.0	2.752787059258146E-5	0.0	5.505574118516292E-5	5.505574118516292E-5
8	0.0	2.752787059258146E-5	0.0	5.505574118516292E-5	5.505574118516292E-5
9	0.0	2.752787059258146E-5	0.0	8.258361177774438E-5	5.505574118516292E-5
10	0.0	2.752787059258146E-5	0.0	1.1011148237032584E-4	5.505574118516292E-5
11	0.0	2.752787059258146E-5	0.0	1.1011148237032584E-4	5.505574118516292E-5
12	2.752787059258146E-5	2.752787059258146E-5	0.0	1.1011148237032584E-4	8.258361177774438E-5
13	2.752787059258146E-5	2.752787059258146E-5	0.0	1.376393529629073E-4	1.376393529629073E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	585	0.0	13.3176985	13
CGTTATT	370	0.0	12.8334055	2
ACGCTTA	180	0.0	12.668928	17
GCGTTAT	385	0.0	12.578857	1
CGCTTAC	205	0.0	12.514602	18
CTCGACG	175	0.0	12.4870825	13
ATCGCCA	655	0.0	12.329596	16
CATCGCC	675	0.0	12.105029	15
GCATCGC	665	0.0	11.858605	14
CGCCAGT	695	0.0	11.757656	18
GTATCAA	5090	0.0	11.715845	1
CGAACGA	325	0.0	11.693588	16
CCGTCGT	695	0.0	11.619658	9
TCGCCAG	690	0.0	11.567282	17
CGTCGTA	695	0.0	11.482956	10
CGACGCT	200	0.0	11.40125	15
AATCGTA	175	1.8189894E-12	11.401249	13
CGGTCCA	750	0.0	11.400934	10
ACGGTAT	665	0.0	11.14377	9
GTTATTC	495	0.0	11.127931	3
>>END_MODULE