FastQCFastQC Report
Thu 2 Feb 2017
SRR4062547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3632682
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA46200.12717876213772633No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC44170.1215906044074323No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT43700.12029679448958097No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA42020.11567211223002728No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT40010.11013901024091843No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG38350.1055693837225499No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC37360.10284412453388433No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA36720.10108234081595911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56250.023.3546311
GTATACG808.979244E-519.2543661
GTGCGAA1355.535003E-817.92579711
AACGCAG76500.017.0247146
TACCGTC11350.016.8633147
GGTATCA28400.016.736191
CGTCGTA11350.016.66948310
ATACCGT12150.016.6583166
TCAACGC79200.016.3887714
TAGTACG4050.016.29732
CGTATAT10300.016.23289310
TATCAAC81800.016.1917082
CAACGCA81100.016.0861975
TGCGTAG4600.015.782495513
ATCAACG82950.015.621563
CCGTCGT12200.015.5080859
GTCGTAG12350.015.31972711
TAAACGC11950.015.28022428
ACCGTCG12400.015.2579548
ATCGTTT13000.015.230659529
Produced by FastQC (version 0.11.5)