##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062547_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3632682
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.330697264445387	32.0	32.0	32.0	32.0	32.0
2	31.43421527125138	32.0	32.0	32.0	32.0	32.0
3	31.512632539814934	32.0	32.0	32.0	32.0	32.0
4	31.60976132785639	32.0	32.0	32.0	32.0	32.0
5	31.550320396885827	32.0	32.0	32.0	32.0	32.0
6	35.146467541061945	36.0	36.0	36.0	36.0	36.0
7	35.15701704690915	36.0	36.0	36.0	36.0	36.0
8	35.10094222395464	36.0	36.0	36.0	36.0	36.0
9	35.23025164327623	36.0	36.0	36.0	36.0	36.0
10	35.10767416470806	36.0	36.0	36.0	36.0	36.0
11	35.24379232754202	36.0	36.0	36.0	36.0	36.0
12	35.1491085649666	36.0	36.0	36.0	36.0	36.0
13	35.197708194661686	36.0	36.0	36.0	36.0	36.0
14	35.15667074629709	36.0	36.0	36.0	36.0	36.0
15	35.11586508260288	36.0	36.0	36.0	36.0	36.0
16	35.127727943156046	36.0	36.0	36.0	36.0	36.0
17	35.094872053210274	36.0	36.0	36.0	36.0	36.0
18	35.114759563319886	36.0	36.0	36.0	36.0	36.0
19	35.10080265765074	36.0	36.0	36.0	36.0	36.0
20	35.095785427956535	36.0	36.0	36.0	36.0	36.0
21	35.09851949606379	36.0	36.0	36.0	36.0	36.0
22	35.07412126907888	36.0	36.0	36.0	36.0	36.0
23	35.03731347803083	36.0	36.0	36.0	36.0	36.0
24	35.01069099910204	36.0	36.0	36.0	36.0	36.0
25	34.989047210848625	36.0	36.0	36.0	36.0	36.0
26	34.95054370297208	36.0	36.0	36.0	32.0	36.0
27	34.93086788218732	36.0	36.0	36.0	32.0	36.0
28	34.9200150192062	36.0	36.0	36.0	32.0	36.0
29	34.8978845382007	36.0	36.0	36.0	32.0	36.0
30	34.88496378158066	36.0	36.0	36.0	32.0	36.0
31	34.890571759377785	36.0	36.0	36.0	32.0	36.0
32	34.85230416535221	36.0	36.0	36.0	32.0	36.0
33	34.84054811293694	36.0	36.0	36.0	32.0	36.0
34	34.828618910215646	36.0	36.0	36.0	32.0	36.0
35	34.8108108554506	36.0	36.0	36.0	32.0	36.0
36	34.78588189112067	36.0	36.0	36.0	32.0	36.0
37	34.78375673951092	36.0	36.0	36.0	32.0	36.0
38	34.74703813876359	36.0	36.0	36.0	32.0	36.0
39	34.74798757502033	36.0	36.0	36.0	32.0	36.0
40	34.74927257601959	36.0	36.0	36.0	32.0	36.0
41	34.710733832468684	36.0	36.0	36.0	32.0	36.0
42	34.69595136596047	36.0	36.0	36.0	32.0	36.0
43	34.69249551708627	36.0	36.0	36.0	32.0	36.0
44	34.65793647778693	36.0	36.0	36.0	32.0	36.0
45	34.63182381502152	36.0	36.0	36.0	32.0	36.0
46	34.64218255272551	36.0	36.0	36.0	32.0	36.0
47	34.620381580331006	36.0	36.0	36.0	32.0	36.0
48	34.58174291060985	36.0	36.0	36.0	32.0	36.0
49	34.57747058509388	36.0	36.0	36.0	32.0	36.0
50	34.06372371707735	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	10.0
20	25.0
21	79.0
22	296.0
23	796.0
24	2163.0
25	4833.0
26	10237.0
27	18856.0
28	31994.0
29	50059.0
30	73678.0
31	108187.0
32	162388.0
33	275875.0
34	630324.0
35	2262882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.795166260294216	17.85938081613684	11.595793769836812	26.749659153732132
2	16.74270651956972	20.247155903951107	36.285338710676456	26.72479886580271
3	18.362567175256054	23.635614874161153	28.539016291634066	29.462801658948727
4	12.600359955228575	15.821430714674644	35.785347102437484	35.79286222765929
5	14.833452207887232	36.23568928843463	33.072350696358974	15.858507807319167
6	34.49856634139574	35.405834395914404	16.507664898267496	13.587934364422363
7	30.726111500630804	30.366762381151762	20.349940085540176	18.55718603267726
8	28.415176445392138	32.21495853476853	19.235705189719333	20.134159830120005
9	27.776337152550102	14.115658898852143	18.369953659582645	39.73805028901511
10	16.323284008894806	25.897642568218192	30.84836492707041	26.930708495816592
11	37.787618073918935	20.96506107608648	21.999365757861547	19.24795509213303
12	25.05944643654468	23.584916048253053	27.93214490010411	23.42349261509816
13	29.464924262569635	18.996240243434464	25.06437392538075	26.474461568615144
14	23.97110454479638	19.635024480535318	24.40502636894724	31.988844605721063
15	25.724932708120335	26.48324295933418	21.765158634859862	26.02666569768562
16	26.547520537167856	25.462702212855405	22.870540278504972	25.119236971471764
17	24.80318948919834	25.316749442973542	24.477617363699878	25.402443704128245
18	25.62621776417534	24.402025831052647	25.393276923220913	24.578479481551096
19	26.190016081782	24.75330898768458	24.759420174956134	24.297254755577285
20	26.226132647999467	23.931244188178322	24.286573941787363	25.55604922203485
21	27.566629834375817	23.788264428320453	23.75258830803247	24.892517429271265
22	26.5076601805498	23.898045576243668	24.213239694528728	25.381054548677806
23	24.960153407317236	23.698220763612117	24.900362872390154	26.44126295668049
24	25.20226102917899	24.78584693072501	24.398667430840355	25.613224609255642
25	25.336431870447235	24.043585428066645	24.754933132049544	25.865049569436575
26	25.07654124418267	24.824468533166407	25.049618986743127	25.049371235907795
27	26.119241926488474	24.33901453526623	23.99191561496437	25.549827923280926
28	25.014823758314105	24.22565476416598	25.187919008600257	25.571602468919657
29	25.07001713885223	24.550758915864368	24.863640693019647	25.515583252263756
30	25.14208510406361	24.583076635940056	25.122788066778213	25.152050193218123
31	25.689366699314718	24.38228834783777	23.984372978422	25.943971974425505
32	25.4337704208626	24.2652948978193	24.15570644499023	26.14522823632787
33	25.201325081578847	23.821160233678587	24.675845559837057	26.30166912490551
34	25.630044138187706	24.13671221428135	25.027679273880842	25.205564373650102
35	26.456761147824114	23.922325158106325	24.617376362698415	25.003537331371145
36	25.129009365532134	24.443014830365005	24.48562797404232	25.942347830060548
37	26.387445969671997	24.234188404049682	24.041410726289833	25.336954899988495
38	25.25695890804645	23.960313619524086	24.919164408004885	25.86356306442458
39	25.616004924185493	24.13274820091602	24.12457242335002	26.12667445154847
40	25.95682748999224	24.17745346275837	24.90308813157882	24.96263091567057
41	24.908345954862	24.6448767054204	25.13005542461465	25.316721915102946
42	26.19981600371296	24.579608124245393	24.623927995899447	24.5966478761422
43	25.32269546302154	23.56427014530862	24.967585932377233	26.14544845929261
44	25.05881329552105	24.108661314147508	24.653162594468768	26.17936279586267
45	25.157720934560196	24.39913540464043	24.889516891376676	25.5536267694227
46	25.02289633787975	24.10665743197097	24.90177354055976	25.96867268958952
47	25.115417511199027	23.927094066338334	25.33442930992289	25.62305911253975
48	25.977555976548455	24.641463249466923	24.39192310254517	24.98905767143945
49	24.793747429585082	24.657374358669433	24.838783025874548	25.710095185870934
50	24.606014125655403	24.900892756616944	24.749296731532443	25.74379638619521
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	14.5
2	23.0
3	85.0
4	147.0
5	182.0
6	217.0
7	237.0
8	257.0
9	343.0
10	429.0
11	636.5
12	844.0
13	1647.0
14	2450.0
15	3439.0
16	4428.0
17	5484.5
18	6541.0
19	7187.5
20	7834.0
21	9510.0
22	11186.0
23	12878.5
24	14571.0
25	19213.5
26	23856.0
27	30190.0
28	36524.0
29	41854.0
30	47184.0
31	50311.0
32	53438.0
33	60959.5
34	68481.0
35	80036.5
36	91592.0
37	112843.5
38	134095.0
39	144820.5
40	155546.0
41	170997.0
42	186448.0
43	187826.0
44	189204.0
45	207171.0
46	225138.0
47	239618.5
48	254099.0
49	262352.0
50	270605.0
51	259396.0
52	248187.0
53	243547.0
54	238907.0
55	245694.0
56	252481.0
57	251433.0
58	250385.0
59	228968.0
60	207551.0
61	183431.0
62	159311.0
63	142941.5
64	126572.0
65	109154.0
66	91736.0
67	84837.5
68	77939.0
69	72686.0
70	67433.0
71	53998.5
72	40564.0
73	36397.5
74	32231.0
75	24387.5
76	16544.0
77	14867.5
78	13191.0
79	11365.0
80	9539.0
81	7528.0
82	5517.0
83	4884.0
84	4251.0
85	3562.5
86	2874.0
87	2227.0
88	1580.0
89	1029.5
90	479.0
91	312.5
92	146.0
93	101.5
94	57.0
95	44.0
96	31.0
97	25.5
98	20.0
99	16.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015030217343549477
2	0.004321875683035289
3	5.505574118516292E-4
4	2.2022296474065168E-4
5	2.752787059258146E-5
6	4.955016706664662E-4
7	8.258361177774437E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.376393529629073E-4
47	2.752787059258146E-5
48	0.0
49	0.0
50	2.752787059258146E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3632682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.180857230523806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.54726209983245	39.122025404109166
2	12.448300211121609	12.244361508917425
3	3.4111939902180186	5.032963338555995
4	1.4282899822840638	2.8097810280999966
5	0.7459383259945304	1.834294315675646
6	0.45898911520177554	1.354408688706178
7	0.3252578663878224	1.1197522482946998
8	0.21983726427755815	0.8649428086706814
9	0.1762130134191784	0.7799676349616089
>10	1.0367011142293623	9.390151810112005
>50	0.0866502915116499	3.0068860297217026
>100	0.09059801139027489	9.224237583230902
>500	0.015389178397086511	5.277364609679125
>1k	0.009379535734756656	7.938862991264806
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	4620	0.12717876213772633	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	4417	0.1215906044074323	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	4370	0.12029679448958097	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	4202	0.11567211223002728	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	4001	0.11013901024091843	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	3835	0.1055693837225499	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	3736	0.10284412453388433	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	3672	0.10108234081595911	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.505574118516292E-5
2	0.0	0.0	0.0	0.0	5.505574118516292E-5
3	0.0	0.0	0.0	0.0	5.505574118516292E-5
4	0.0	0.0	0.0	2.752787059258146E-5	5.505574118516292E-5
5	0.0	2.752787059258146E-5	0.0	5.505574118516292E-5	5.505574118516292E-5
6	0.0	2.752787059258146E-5	0.0	5.505574118516292E-5	5.505574118516292E-5
7	0.0	2.752787059258146E-5	0.0	8.258361177774438E-5	5.505574118516292E-5
8	0.0	2.752787059258146E-5	0.0	8.258361177774438E-5	8.258361177774438E-5
9	0.0	2.752787059258146E-5	0.0	1.1011148237032584E-4	8.258361177774438E-5
10	0.0	2.752787059258146E-5	0.0	1.376393529629073E-4	8.258361177774438E-5
11	0.0	2.752787059258146E-5	0.0	1.9269509414807022E-4	8.258361177774438E-5
12	0.0	2.752787059258146E-5	0.0	2.2022296474065168E-4	2.477508353332331E-4
13	0.0	2.752787059258146E-5	0.0	2.2022296474065168E-4	2.752787059258146E-4
14	0.0	2.752787059258146E-5	0.0	2.477508353332331E-4	2.752787059258146E-4
15	0.0	2.752787059258146E-5	0.0	2.752787059258146E-4	3.303344471109775E-4
16	0.0	2.752787059258146E-5	0.0	3.5786231770355896E-4	3.8539018829614045E-4
17	0.0	2.752787059258146E-5	0.0	3.5786231770355896E-4	3.8539018829614045E-4
18	0.0	2.752787059258146E-5	0.0	3.8539018829614045E-4	3.8539018829614045E-4
19	0.0	2.752787059258146E-5	0.0	3.8539018829614045E-4	3.8539018829614045E-4
20	0.0	2.752787059258146E-5	0.0	4.679738000738848E-4	5.230295412590477E-4
21	0.0	2.752787059258146E-5	0.0	5.230295412590477E-4	5.230295412590477E-4
22	0.0	2.752787059258146E-5	0.0	6.056131530367921E-4	5.230295412590477E-4
23	0.0	2.752787059258146E-5	0.0	0.001018531211925514	5.230295412590477E-4
24	0.0	2.752787059258146E-5	0.0	0.0014314492708142358	5.230295412590477E-4
25	0.0	2.752787059258146E-5	0.0	0.0018718952002955392	5.230295412590477E-4
26	0.0	2.752787059258146E-5	0.0	0.002284813259184261	5.505574118516292E-4
27	0.0	2.752787059258146E-5	0.0	0.0030280657651839604	5.505574118516292E-4
28	0.0	2.752787059258146E-5	0.0	0.007047134871700854	5.505574118516292E-4
29	0.0	2.752787059258146E-5	0.0	0.014975161602364314	5.505574118516292E-4
30	0.0	2.752787059258146E-5	0.0	0.031574467569690935	5.505574118516292E-4
31	5.505574118516292E-5	2.752787059258146E-5	0.0	0.081950470754115	6.331410236293736E-4
32	5.505574118516292E-5	2.752787059258146E-5	0.0	0.13684104471572242	6.331410236293736E-4
33	5.505574118516292E-5	2.752787059258146E-5	0.0	0.19330070730110702	6.331410236293736E-4
34	5.505574118516292E-5	2.752787059258146E-5	0.0	0.25829400977019185	6.331410236293736E-4
35	5.505574118516292E-5	2.752787059258146E-5	0.0	0.3278294108870526	6.331410236293736E-4
36	5.505574118516292E-5	2.752787059258146E-5	0.0	0.4305083681973814	6.331410236293736E-4
37	5.505574118516292E-5	2.752787059258146E-5	0.0	0.5782779775383587	6.331410236293736E-4
38	5.505574118516292E-5	2.752787059258146E-5	0.0	0.7821769150176096	6.60668894221955E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5625	0.0	23.354631	1
GTATACG	80	8.979244E-5	19.254366	1
GTGCGAA	135	5.535003E-8	17.925797	11
AACGCAG	7650	0.0	17.024714	6
TACCGTC	1135	0.0	16.863314	7
GGTATCA	2840	0.0	16.73619	1
CGTCGTA	1135	0.0	16.669483	10
ATACCGT	1215	0.0	16.658316	6
TCAACGC	7920	0.0	16.388771	4
TAGTACG	405	0.0	16.2973	2
CGTATAT	1030	0.0	16.232893	10
TATCAAC	8180	0.0	16.191708	2
CAACGCA	8110	0.0	16.086197	5
TGCGTAG	460	0.0	15.7824955	13
ATCAACG	8295	0.0	15.62156	3
CCGTCGT	1220	0.0	15.508085	9
GTCGTAG	1235	0.0	15.319727	11
TAAACGC	1195	0.0	15.280224	28
ACCGTCG	1240	0.0	15.257954	8
ATCGTTT	1300	0.0	15.2306595	29
>>END_MODULE