FastQCFastQC Report
Thu 2 Feb 2017
SRR4062546_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062546_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862225
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32440.37623590130186435No Hit
GTACATGGAAGCAGTGGTATCAACG29270.3394705558293949No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24570.2849604221635884No Hit
TCCATGTACTCTGCGTTGATACCAC22350.2592130824320798No Hit
TATCAACGCAGAGTACTTTTTTTTT21500.2493548667691148No Hit
ATTCCATTCCATTCCATTCCATTCC20170.23392965873176957No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19160.22221577894401112No Hit
GAATGGAATGGAATGGAATGGAATG17340.20110759952448606No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13630.1580793876308388No Hit
GAGTACATGGAAGCAGTGGTATCAA12920.14984487807706803No Hit
GGTATCAACGCAGAGTACTTTTTTT11340.1315201948447331No Hit
GTACATGGGGTGGTATCAACGCAAA10320.1196903360491751No Hit
GAATAGGACCGCGGTTCTATTTTGT10250.11887848299457798No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10170.11795065093218127No Hit
GTCTTGCGCCGGTCCAAGAATTTCA10120.11737075589318333No Hit
CATGTACTCTGCGTTGATACCACTG10100.11713879787758415No Hit
GCGCAAGACGGACCAGAGCGAAAGC9740.11296355359679898No Hit
GAATAACGCCGCCGCATCGCCAGTC9690.11238365855780104No Hit
GAACTACGACGGTATCTGATCGTCT9190.10658470816782162No Hit
GATTAAGAGGGACGGCCGGGGGCAT9100.10554089709762532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT250.006041765718.9935571
TCACGTA551.1264254E-515.546515
ATATTAC508.731977E-515.1948451
GCGTTAT602.5738585E-514.2451681
ACGGTAT1550.014.0977229
CGTTATT551.9641987E-413.8134962
GAACCGC707.248518E-613.5723416
TATTCCG707.2572184E-613.5707655
TCGACGC500.001497841413.30089514
CGTAGGA655.4405813E-513.15549518
AACCGCG655.4435466E-513.1547317
GTTATTC655.461369E-513.1501493
CGGTTCT2000.012.82660812
ACTGTCC604.0864173E-412.6675178
AGAACCG751.4775143E-512.6660475
CGAACGT905.4025986E-712.6653124
GATATAC751.48193085E-512.6623711
GTCTTCG1457.2759576E-1212.44911311
GGTTCTA2150.012.37292513
AAGACGG2150.012.3714885
Produced by FastQC (version 0.11.5)