##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062546_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	862225
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.052231146162544	32.0	32.0	32.0	32.0	32.0
2	30.767658093885007	32.0	32.0	32.0	32.0	32.0
3	30.77383745542057	32.0	32.0	32.0	32.0	32.0
4	30.80293890805764	32.0	32.0	32.0	32.0	32.0
5	30.618420945808808	32.0	32.0	32.0	32.0	32.0
6	34.25305111774769	36.0	36.0	36.0	32.0	36.0
7	34.113937777262315	36.0	36.0	36.0	32.0	36.0
8	34.063282785815765	36.0	36.0	36.0	32.0	36.0
9	34.21922468033286	36.0	36.0	36.0	32.0	36.0
10	33.91481921772159	36.0	36.0	36.0	32.0	36.0
11	34.256157035576564	36.0	36.0	36.0	32.0	36.0
12	34.0478482994578	36.0	36.0	36.0	32.0	36.0
13	34.15940734727015	36.0	36.0	36.0	32.0	36.0
14	34.05777726231552	36.0	36.0	36.0	32.0	36.0
15	34.01550523355273	36.0	36.0	36.0	32.0	36.0
16	33.99306677491374	36.0	36.0	36.0	32.0	36.0
17	33.94654643509525	36.0	36.0	36.0	32.0	36.0
18	33.95536083968802	36.0	36.0	36.0	32.0	36.0
19	33.93043115196149	36.0	36.0	36.0	32.0	36.0
20	33.88402099220041	36.0	36.0	36.0	32.0	36.0
21	33.838377453680884	36.0	36.0	36.0	32.0	36.0
22	33.83858041694453	36.0	36.0	36.0	32.0	36.0
23	33.78727478326423	36.0	36.0	36.0	32.0	36.0
24	33.73692829597843	36.0	36.0	36.0	27.0	36.0
25	33.27751456986285	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	6.0
4	35.0
5	73.0
6	152.0
7	42.0
8	129.0
9	184.0
10	97.0
11	40.0
12	73.0
13	53.0
14	270.0
15	273.0
16	365.0
17	573.0
18	723.0
19	1052.0
20	1476.0
21	2213.0
22	3181.0
23	4510.0
24	6213.0
25	8192.0
26	11279.0
27	14204.0
28	18861.0
29	24460.0
30	31263.0
31	42367.0
32	60878.0
33	86585.0
34	191320.0
35	351081.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.40320689259207	18.442300175260947	10.841472934304402	24.313019997842584
2	16.218623650825684	21.26597958232138	37.655446454046306	24.85995031280663
3	18.870769444895497	26.62031130390406	28.11907026375003	26.389848987450414
4	12.215537050505333	14.764408594292819	36.25141658750016	36.76863776770169
5	15.10227285911522	36.26921604343841	32.3107981111717	16.317712986274667
6	33.82516241847167	35.74343509120846	16.7120165882084	13.719385902111469
7	28.499437100312207	30.93127981337264	22.627522893188335	17.94176019312682
8	27.6512236148791	32.66165403059821	20.82983621287103	18.857286141651663
9	27.030883382462378	13.769639525528335	19.893258968324826	39.30621812368445
10	16.7232570021831	26.006897672906316	30.475869757071855	26.79397556783873
11	37.24292499390336	22.79771927583524	21.229314969865175	18.730040760396225
12	23.72761280267212	24.724083345818247	29.80225053685141	21.74605331465822
13	28.520117194984724	20.916030002473494	26.249841777480494	24.314011025061287
14	23.284136398106135	19.15463646952666	25.873043338903745	31.688183793463452
15	25.44389210730745	26.4838496543732	23.109212960221274	24.963045278098072
16	26.26678881995511	26.800454707488157	23.378537016974896	23.554219455581837
17	22.777910035245004	26.377777648746104	26.994187768184275	23.85012454782462
18	23.867253687558865	25.80344710250374	27.76309967470743	22.566199535229963
19	24.52073551289749	24.53211684681305	26.76088398123706	24.186263659052397
20	25.688947003540687	23.775421450577323	25.82632415666337	24.709307389218623
21	27.286927578545907	25.1859052304319	23.607264095176046	23.919903095846152
22	24.437727818678983	25.334947182076057	26.563687009369463	23.6636379898755
23	23.01548124978948	25.584539424398095	26.635787018700846	24.76419230711157
24	23.476915643844016	24.818586414189745	26.554442658440724	25.150055283525507
25	24.87218914844641	24.46384480127474	25.478782585669858	25.185183464608997
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	286.0
1	286.0
2	548.5
3	811.0
4	811.0
5	811.0
6	2225.0
7	3639.0
8	3639.0
9	3639.0
10	3657.5
11	3676.0
12	3676.0
13	3676.0
14	3253.5
15	2831.0
16	2831.0
17	2831.0
18	4593.5
19	6356.0
20	6356.0
21	6356.0
22	10086.0
23	13816.0
24	13816.0
25	13816.0
26	20481.5
27	27147.0
28	27147.0
29	27147.0
30	34167.5
31	41188.0
32	41188.0
33	41188.0
34	51370.0
35	61552.0
36	61552.0
37	61552.0
38	72021.5
39	82491.0
40	82491.0
41	82491.0
42	87636.0
43	92781.0
44	92781.0
45	92781.0
46	104380.0
47	115979.0
48	115979.0
49	115979.0
50	111231.0
51	106483.0
52	106483.0
53	106483.0
54	95686.5
55	84890.0
56	84890.0
57	84890.0
58	78010.5
59	71131.0
60	71131.0
61	71131.0
62	64454.0
63	57777.0
64	57777.0
65	57777.0
66	49274.5
67	40772.0
68	40772.0
69	40772.0
70	31305.0
71	21838.0
72	21838.0
73	21838.0
74	17329.0
75	12820.0
76	12820.0
77	12820.0
78	10401.5
79	7983.0
80	7983.0
81	7983.0
82	5650.0
83	3317.0
84	3317.0
85	3317.0
86	2437.5
87	1558.0
88	1558.0
89	1558.0
90	1059.0
91	560.0
92	560.0
93	560.0
94	339.0
95	118.0
96	118.0
97	118.0
98	271.5
99	425.0
100	425.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009510278639566239
2	0.003595349241787237
3	0.005219055350981472
4	0.013685522920351417
5	0.036533387456870306
6	0.047319435182232014
7	0.07132708979674679
8	0.09301516425526979
9	0.10298935892603439
10	0.12351764330656152
11	0.12699701354054915
12	0.1375511032503117
13	0.12711299254834876
14	0.13082432079793557
15	0.12015425208037345
16	0.11737075589318333
17	0.128736698657543
18	0.13383977500072486
19	0.13523152309431993
20	0.1266490765171504
21	0.1354634811099191
22	0.15575980747484705
23	0.14474180173388618
24	0.1402186204297022
25	0.13766708225811128
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	862225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.89034183180231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16997654695477	37.43586897817634
2	12.930100227438329	12.384538396229727
3	3.7620964961656123	5.405042616167908
4	1.4952198714547738	2.8642636303069047
5	0.809402678976523	1.9381285487881115
6	0.5004921054147271	1.4381242807457832
7	0.35036622356717595	1.174541074906481
8	0.2583115159074463	0.9896501436718915
9	0.19432290739252361	0.8375571414699858
>10	1.2242574706182987	11.453408026991834
>50	0.156375293682204	5.306141129637294
>100	0.1371620972424621	12.948104077586898
>500	0.008025441859386946	2.6142186492797896
>1k	0.0038911233257633683	3.21041330604105
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3244	0.37623590130186435	No Hit
GTACATGGAAGCAGTGGTATCAACG	2927	0.3394705558293949	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2457	0.2849604221635884	No Hit
TCCATGTACTCTGCGTTGATACCAC	2235	0.2592130824320798	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2150	0.2493548667691148	No Hit
ATTCCATTCCATTCCATTCCATTCC	2017	0.23392965873176957	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1916	0.22221577894401112	No Hit
GAATGGAATGGAATGGAATGGAATG	1734	0.20110759952448606	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1363	0.1580793876308388	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1292	0.14984487807706803	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1134	0.1315201948447331	No Hit
GTACATGGGGTGGTATCAACGCAAA	1032	0.1196903360491751	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1025	0.11887848299457798	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1017	0.11795065093218127	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1012	0.11737075589318333	No Hit
CATGTACTCTGCGTTGATACCACTG	1010	0.11713879787758415	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	974	0.11296355359679898	No Hit
GAATAACGCCGCCGCATCGCCAGTC	969	0.11238365855780104	No Hit
GAACTACGACGGTATCTGATCGTCT	919	0.10658470816782162	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	910	0.10554089709762532	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1597900779958827E-4
2	0.0	0.0	0.0	0.0	1.1597900779958827E-4
3	0.0	0.0	0.0	0.0	1.1597900779958827E-4
4	0.0	0.0	0.0	0.0	1.1597900779958827E-4
5	0.0	0.0	0.0	0.0	1.1597900779958827E-4
6	0.0	0.0	0.0	0.0	1.1597900779958827E-4
7	0.0	0.0	0.0	0.0	1.1597900779958827E-4
8	0.0	0.0	0.0	0.0	1.1597900779958827E-4
9	0.0	0.0	0.0	1.1597900779958827E-4	3.4793702339876485E-4
10	0.0	0.0	0.0	1.1597900779958827E-4	3.4793702339876485E-4
11	0.0	0.0	0.0	1.1597900779958827E-4	3.4793702339876485E-4
12	0.0	0.0	0.0	2.3195801559917654E-4	8.118530545971179E-4
13	0.0	0.0	0.0	4.6391603119835307E-4	8.118530545971179E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAT	25	0.0060417657	18.993557	1
TCACGTA	55	1.1264254E-5	15.5465	15
ATATTAC	50	8.731977E-5	15.194845	1
GCGTTAT	60	2.5738585E-5	14.245168	1
ACGGTAT	155	0.0	14.097722	9
CGTTATT	55	1.9641987E-4	13.813496	2
GAACCGC	70	7.248518E-6	13.572341	6
TATTCCG	70	7.2572184E-6	13.570765	5
TCGACGC	50	0.0014978414	13.300895	14
CGTAGGA	65	5.4405813E-5	13.155495	18
AACCGCG	65	5.4435466E-5	13.154731	7
GTTATTC	65	5.461369E-5	13.150149	3
CGGTTCT	200	0.0	12.826608	12
ACTGTCC	60	4.0864173E-4	12.667517	8
AGAACCG	75	1.4775143E-5	12.666047	5
CGAACGT	90	5.4025986E-7	12.665312	4
GATATAC	75	1.48193085E-5	12.662371	1
GTCTTCG	145	7.2759576E-12	12.449113	11
GGTTCTA	215	0.0	12.372925	13
AAGACGG	215	0.0	12.371488	5
>>END_MODULE