FastQCFastQC Report
Thu 2 Feb 2017
SRR4062546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862225
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA12340.14311809562469194No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10500.12177795818956769No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10270.11911044101017716No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10150.11771869291658212No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10140.1176027139087825No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG9810.1137754066513961No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9460.1097161413784105No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9020.10461306503522863No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA8820.10229348487923685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT407.030919E-427.499716
GACGCAA508.339302E-526.39972324
GTATTAC901.5077603E-824.449861
CGCGCCA752.0633142E-623.46642110
CGTGCCG500.002580000821.9997710
GTGTCGA500.002580000821.9997711
ACGTATG803.6138845E-621.9997732
CGCAATA2350.021.53168936
CCTTACA1900.020.8443032
AACGTAT755.4707452E-520.53311731
CAACGTA755.4707452E-520.53311730
GGCGAAT755.4707452E-520.53311737
TATTCGA654.930936E-420.30747816
ATACGAA2500.020.23978840
ATGCCGG1201.4302714E-820.16645419
TAGGACG1750.020.115244
CTTATAT550.00447684620.0044291
GACGAAA2100.019.90455222
CTTACAC1900.019.6851443
GATATAC2150.019.4461671
Produced by FastQC (version 0.11.5)