##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062546_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 862225 Sequences flagged as poor quality 0 Sequence length 50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19553248861956 32.0 32.0 32.0 32.0 32.0 2 31.413939516947433 32.0 32.0 32.0 32.0 32.0 3 31.508451970193395 32.0 32.0 32.0 32.0 32.0 4 31.61049841978602 32.0 32.0 32.0 32.0 32.0 5 31.54597117921656 32.0 32.0 32.0 32.0 32.0 6 35.1282646640958 36.0 36.0 36.0 36.0 36.0 7 35.15256574560004 36.0 36.0 36.0 36.0 36.0 8 35.078919075647306 36.0 36.0 36.0 36.0 36.0 9 35.19075879265853 36.0 36.0 36.0 36.0 36.0 10 35.08958798457479 36.0 36.0 36.0 36.0 36.0 11 35.208401519325 36.0 36.0 36.0 36.0 36.0 12 35.12616544405463 36.0 36.0 36.0 36.0 36.0 13 35.152002087622144 36.0 36.0 36.0 36.0 36.0 14 35.12409173939517 36.0 36.0 36.0 36.0 36.0 15 35.08884803850503 36.0 36.0 36.0 36.0 36.0 16 35.09214764127693 36.0 36.0 36.0 36.0 36.0 17 35.07846559772681 36.0 36.0 36.0 36.0 36.0 18 35.08359418945171 36.0 36.0 36.0 36.0 36.0 19 35.04846646756937 36.0 36.0 36.0 36.0 36.0 20 35.03316999623068 36.0 36.0 36.0 36.0 36.0 21 34.99990953637391 36.0 36.0 36.0 36.0 36.0 22 35.00350140624547 36.0 36.0 36.0 36.0 36.0 23 34.96209110151062 36.0 36.0 36.0 32.0 36.0 24 34.91879961726927 36.0 36.0 36.0 32.0 36.0 25 34.902612427150686 36.0 36.0 36.0 32.0 36.0 26 34.84147641276929 36.0 36.0 36.0 32.0 36.0 27 34.84912522833367 36.0 36.0 36.0 32.0 36.0 28 34.80880396648207 36.0 36.0 36.0 32.0 36.0 29 34.759633506335355 36.0 36.0 36.0 32.0 36.0 30 34.7484624083041 36.0 36.0 36.0 32.0 36.0 31 34.7186929165821 36.0 36.0 36.0 32.0 36.0 32 34.70300095682681 36.0 36.0 36.0 32.0 36.0 33 34.65656528168402 36.0 36.0 36.0 32.0 36.0 34 34.60360462756241 36.0 36.0 36.0 32.0 36.0 35 34.5524891994549 36.0 36.0 36.0 32.0 36.0 36 34.51237090086694 36.0 36.0 36.0 32.0 36.0 37 34.51982487169822 36.0 36.0 36.0 32.0 36.0 38 34.44985009713242 36.0 36.0 36.0 32.0 36.0 39 34.38538664501725 36.0 36.0 36.0 32.0 36.0 40 34.35817217083708 36.0 36.0 36.0 32.0 36.0 41 34.3060164110296 36.0 36.0 36.0 32.0 36.0 42 34.278739308185216 36.0 36.0 36.0 32.0 36.0 43 34.25926063382528 36.0 36.0 36.0 32.0 36.0 44 34.17244918669721 36.0 36.0 36.0 32.0 36.0 45 34.0947003392386 36.0 36.0 36.0 32.0 36.0 46 34.10314477079648 36.0 36.0 36.0 32.0 36.0 47 34.081871901185885 36.0 36.0 36.0 32.0 36.0 48 34.00727768273942 36.0 36.0 36.0 32.0 36.0 49 33.960556699237436 36.0 36.0 36.0 32.0 36.0 50 33.461089622778275 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 4.0 20 5.0 21 32.0 22 101.0 23 240.0 24 705.0 25 1683.0 26 3522.0 27 6546.0 28 10692.0 29 16219.0 30 22531.0 31 31734.0 32 45756.0 33 70443.0 34 147304.0 35 504707.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80182716410258 18.92075290482206 11.348090304964268 24.929329626111095 2 15.72239143193169 21.667335515404012 37.245441196597454 25.364831856066843 3 18.164927362094684 26.760045556712775 28.504375903189665 26.57065117800288 4 12.011147915158936 15.133770342741562 36.43705116071926 36.41803058138024 5 14.89332830757633 36.25051465684711 32.351068456609354 16.505088578967207 6 34.08325707678193 35.88778283061999 16.390809316868875 13.638150775729194 7 28.459624807909766 30.840673838035315 22.618921975122504 18.080779378932412 8 27.392038041114557 33.00542201861463 20.705500304444897 18.897039635825916 9 26.969294557685057 13.531154860970165 19.824059845168023 39.67549073617675 10 16.657253037200267 26.03682333497637 30.502247093276118 26.80367653454725 11 37.46701846965699 22.59433442546899 21.220447099075066 18.718200005798952 12 23.707848879352834 24.66722723187103 29.777494273536494 21.84742961523964 13 28.662588071559046 20.826234451564265 26.289077676940476 24.22209979993621 14 23.167502682014554 18.93577662443098 26.063266548754676 31.833454144799788 15 25.580098002261593 26.386732001507728 23.065905071182115 24.967264925048564 16 26.333729594943318 26.626808547652875 23.34622633303372 23.693235524370092 17 22.888225231233147 26.276435965090318 26.93809620458697 23.897242599089566 18 23.91869871553249 25.53312650410276 27.86801588912407 22.680158891240684 19 24.725332714778624 24.20887819304706 26.953057496593118 24.1127315955812 20 25.790135985386648 23.3239583635362 25.979413726115574 24.906491924961582 21 27.354808779610888 25.02548638696396 23.82278407608223 23.79692075734292 22 24.532923540839107 25.109455188610863 26.686769694685264 23.670851575864766 23 23.03667836121662 25.30882310301835 26.75844472150541 24.89605381425962 24 23.519035054655106 24.5643538519528 26.666705326335933 25.249905767056163 25 25.062367711444228 24.010438110701962 25.685870857374816 25.24132332047899 26 24.524920989300934 25.998550262402503 25.71567746237931 23.760851285917248 27 24.26999913015744 25.629852996607617 26.69245266606744 23.407695207167503 28 23.157992403374987 25.618487053843253 27.223636521789558 23.9998840209922 29 23.133520832729275 24.158195366638637 27.55174113485459 25.156542665777494 30 24.672330308214214 24.623735103946185 26.31250543651599 24.39142915132361 31 24.904172344805588 25.81901475832874 24.79654382556757 24.480269071298093 32 23.20264432137783 25.73249441851025 26.835802719707736 24.229058540404186 33 23.02589231349126 25.64539418365276 26.843109397199104 24.485604105656876 34 23.793209429093334 24.09881411464525 27.43842964423439 24.669546812027026 35 25.860303285105395 24.322653599698455 25.63611586302879 24.180927252167358 36 24.692162718547944 26.34323987358288 24.9861695033199 23.978427904549278 37 24.39131317231581 25.82005856939894 26.55142219258314 23.237206065702107 38 23.437153875148596 26.01072805822146 26.486937864246574 24.065180202383367 39 23.94618574038099 23.97715213546348 26.46432195772565 25.612340166429874 40 25.319377192728115 24.74609295717475 25.616167473687263 24.31836237640987 41 24.296558322943547 26.210675867667955 25.606309258024297 23.8864565513642 42 24.49789788048363 26.221461915393313 26.692800603090838 22.587839601032215 43 23.611934239902578 25.60990460726609 26.388587665632517 24.389573487198817 44 23.27385543216678 24.43851662849024 26.75450143524022 25.53312650410276 45 24.476557743048506 25.135898402389167 25.540549160601934 24.846994693960394 46 24.479137671881087 25.88631260554102 24.961610903895043 24.672938818682848 47 23.50526081389523 25.45846554955557 27.190730019113364 23.84554361743584 48 23.863492707819884 27.24254110006089 25.396503232914842 23.497462959204384 49 23.116239960567135 24.81939169010409 26.72040360694714 25.34396474238163 50 23.9145234712517 26.10652671866392 24.834932877149235 25.144016932935138 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 45.0 1 49.0 2 53.0 3 160.5 4 268.0 5 301.5 6 335.0 7 316.0 8 297.0 9 366.0 10 435.0 11 665.5 12 896.0 13 1471.0 14 2046.0 15 2783.5 16 3521.0 17 4034.0 18 4547.0 19 4839.5 20 5132.0 21 5718.5 22 6305.0 23 6358.0 24 6411.0 25 6898.5 26 7386.0 27 8702.5 28 10019.0 29 11225.0 30 12431.0 31 13587.0 32 14743.0 33 17185.0 34 19627.0 35 23426.0 36 27225.0 37 33706.0 38 40187.0 39 43484.0 40 46781.0 41 49731.0 42 52681.0 43 51616.0 44 50551.0 45 54120.0 46 57689.0 47 59665.5 48 61642.0 49 60070.0 50 58498.0 51 54530.0 52 50562.0 53 49358.0 54 48154.0 55 49494.0 56 50834.0 57 50703.5 58 50573.0 59 46758.0 60 42943.0 61 38292.5 62 33642.0 63 29825.5 64 26009.0 65 22395.5 66 18782.0 67 17160.5 68 15539.0 69 14421.0 70 13303.0 71 10366.0 72 7429.0 73 6650.0 74 5871.0 75 4284.5 76 2698.0 77 2405.0 78 2112.0 79 1826.0 80 1540.0 81 1224.5 82 909.0 83 817.0 84 725.0 85 618.0 86 511.0 87 365.0 88 219.0 89 144.5 90 70.0 91 48.0 92 26.0 93 18.5 94 11.0 95 8.0 96 5.0 97 4.0 98 3.0 99 3.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014381396967148947 2 0.005451013366580649 3 3.479370233987648E-4 4 1.1597900779958827E-4 5 0.0 6 4.6391603119835307E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 1.1597900779958827E-4 47 1.1597900779958827E-4 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 862225.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.11854651796647 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35114396286048 42.94368575807919 2 12.583010999960228 13.619485322748629 3 3.5377070279759124 5.74366687081354 4 1.4176426278297813 3.0688303400023305 5 0.7432962113613459 2.011305529559362 6 0.4423342902268371 1.436309331727966 7 0.3111610083841687 1.1787707054771193 8 0.23265735097585233 1.0072862137226812 9 0.17468422164705164 0.8508290557644658 >10 0.9860819004964219 10.275327089486467 >50 0.11165007263148403 4.249682407941846 >100 0.10151762576169694 10.763897376119747 >500 0.006035018087425756 2.2280389221566037 >1k 0.001077681801326028 0.6228850763999929 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 1234 0.14311809562469194 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC 1050 0.12177795818956769 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 1027 0.11911044101017716 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT 1015 0.11771869291658212 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 1014 0.1176027139087825 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG 981 0.1137754066513961 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 946 0.1097161413784105 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 902 0.10461306503522863 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA 882 0.10229348487923685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 1.1597900779958827E-4 3.4793702339876485E-4 13 0.0 0.0 0.0 3.4793702339876485E-4 4.6391603119835307E-4 14 0.0 0.0 0.0 3.4793702339876485E-4 4.6391603119835307E-4 15 0.0 0.0 0.0 3.4793702339876485E-4 4.6391603119835307E-4 16 0.0 0.0 0.0 3.4793702339876485E-4 6.958740467975297E-4 17 0.0 0.0 0.0 3.4793702339876485E-4 6.958740467975297E-4 18 0.0 0.0 0.0 3.4793702339876485E-4 6.958740467975297E-4 19 0.0 0.0 0.0 5.798950389979414E-4 6.958740467975297E-4 20 0.0 0.0 0.0 9.278320623967061E-4 6.958740467975297E-4 21 1.1597900779958827E-4 0.0 0.0 0.0019716431325930007 8.118530545971179E-4 22 1.1597900779958827E-4 0.0 0.0 0.0032474122183884717 8.118530545971179E-4 23 1.1597900779958827E-4 0.0 0.0 0.006494824436776943 9.278320623967061E-4 24 1.1597900779958827E-4 0.0 0.0 0.010206152686363767 9.278320623967061E-4 25 1.1597900779958827E-4 0.0 0.0 0.013337585896952652 9.278320623967061E-4 26 1.1597900779958827E-4 0.0 0.0 0.017048914146539477 9.278320623967061E-4 27 1.1597900779958827E-4 0.0 0.0 0.021920032474122185 9.278320623967061E-4 28 1.1597900779958827E-4 0.0 0.0 0.034445765316477715 9.278320623967061E-4 29 1.1597900779958827E-4 0.0 0.0 0.050798805416219665 9.278320623967061E-4 30 1.1597900779958827E-4 0.0 0.0 0.08582446577169532 9.278320623967061E-4 31 1.1597900779958827E-4 0.0 0.0 0.14532169677288412 9.278320623967061E-4 32 1.1597900779958827E-4 0.0 0.0 0.21838847168662473 0.0010438110701962945 33 1.1597900779958827E-4 0.0 0.0 0.2878598973585781 0.0010438110701962945 34 1.1597900779958827E-4 0.0 0.0 0.3705529299196845 0.0010438110701962945 35 1.1597900779958827E-4 0.0 0.0 0.4724984777755226 0.0011597900779958827 36 1.1597900779958827E-4 0.0 0.0 0.5926527298558961 0.0011597900779958827 37 1.1597900779958827E-4 0.0 0.0 0.7746237930934501 0.0011597900779958827 38 1.1597900779958827E-4 0.0 0.0 0.9964916350140625 0.0011597900779958827 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCGT 40 7.030919E-4 27.49971 6 GACGCAA 50 8.339302E-5 26.399723 24 GTATTAC 90 1.5077603E-8 24.44986 1 CGCGCCA 75 2.0633142E-6 23.466421 10 CGTGCCG 50 0.0025800008 21.99977 10 GTGTCGA 50 0.0025800008 21.99977 11 ACGTATG 80 3.6138845E-6 21.99977 32 CGCAATA 235 0.0 21.531689 36 CCTTACA 190 0.0 20.844303 2 AACGTAT 75 5.4707452E-5 20.533117 31 CAACGTA 75 5.4707452E-5 20.533117 30 GGCGAAT 75 5.4707452E-5 20.533117 37 TATTCGA 65 4.930936E-4 20.307478 16 ATACGAA 250 0.0 20.239788 40 ATGCCGG 120 1.4302714E-8 20.166454 19 TAGGACG 175 0.0 20.11524 4 CTTATAT 55 0.004476846 20.004429 1 GACGAAA 210 0.0 19.904552 22 CTTACAC 190 0.0 19.685144 3 GATATAC 215 0.0 19.446167 1 >>END_MODULE