FastQCFastQC Report
Thu 2 Feb 2017
SRR4062545_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062545_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041983
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG150081.4403306004032697No Hit
TCCATGTACTCTGCGTTGATACCAC126741.216334623501535No Hit
GAGTACATGGAAGCAGTGGTATCAA69670.6686289507602331No Hit
CATGTACTCTGCGTTGATACCACTG59030.5665159604331357No Hit
GCTTCCATGTACTCTGCGTTGATAC40510.3887779359164209No Hit
CCCATGTACTCTGCGTTGATACCAC39280.3769735206812395No Hit
GTACATGGGAAGCAGTGGTATCAAC36680.35202109823288863No Hit
GCGTTGATACCACTGCTTCCATGTA34010.32639687979554366No Hit
CATGGAAGCAGTGGTATCAACGCAG33560.3220781912948676No Hit
GTATCAACGCAGAGTACTTTTTTTT31370.3010605739249105No Hit
GTATCAACGCAGAGTACATGGAAGC25920.24875645763894422No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25670.2463571862496797No Hit
ACTCTGCGTTGATACCACTGCTTCC24650.2365681589814805No Hit
ACGCAGAGTACATGGAAGCAGTGGT23300.22361209347945216No Hit
TATCAACGCAGAGTACTTTTTTTTT22950.22025311353448185No Hit
CAGTGGTATCAACGCAGAGTACATG22760.21842966727864083No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20310.19491680766384864No Hit
TATCAACGCAGAGTACATGGAAGCA16650.15979147452501624No Hit
GTATCAACGCAGAGTACATGGGAAG16210.1555687568799107No Hit
GTACATGGGGTGGTATCAACGCAAA15260.1464515256007056No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15090.14482002105600572No Hit
GAGTACATGGGAAGCAGTGGTATCA12900.12380240368604861No Hit
ATACCACTGCTTCCATGTACTCTGC12520.12015551117436657No Hit
GGTATCAACGCAGAGTACATGGAAG12100.1161247352404022No Hit
AAGCAGTGGTATCAACGCAGAGTAC11680.11209395930643783No Hit
GCGTTGATACCACTGCTTCCCATGT11250.10796721251690286No Hit
GGTATCAACGCAGAGTACTTTTTTT11190.10739138738347939No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG350.002178623316.2755431
GCGTTAT651.9251274E-716.0668831
TTAGGAC1250.015.1934273
CCCTTAT456.791764E-414.7685491
CGTTATT653.3897595E-614.6069592
TAGGACG1450.014.4110224
CAACGGA602.5643238E-514.25136914
ACAGTCG400.00527586614.2506868
AAAGGCG400.005280916614.2486315
TCGCCAG1400.013.57599717
CGAGGTT852.7024362E-713.4091874
TTCGAAG500.001505494613.2923332
AACGGAC655.4417356E-513.15574415
TTAGGCA957.396011E-812.99754
GTTATTC957.419294E-812.9943783
CGCCAGT1650.012.67153918
TACCTAA604.0784082E-412.6709319
TTTAGGA1600.012.4615622
CTTCCGT1001.4379293E-712.35178215
GGACGTG1551.8189894E-1212.2586546
Produced by FastQC (version 0.11.5)