##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062545_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041983
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.079080944698713	32.0	32.0	32.0	32.0	32.0
2	30.80827038444965	32.0	32.0	32.0	32.0	32.0
3	30.799593659397516	32.0	32.0	32.0	32.0	32.0
4	30.84489862118672	32.0	32.0	32.0	32.0	32.0
5	30.689453666710495	32.0	32.0	32.0	32.0	32.0
6	34.31978448784673	36.0	36.0	36.0	32.0	36.0
7	34.17272546673026	36.0	36.0	36.0	32.0	36.0
8	34.14682773135454	36.0	36.0	36.0	32.0	36.0
9	34.29442706838787	36.0	36.0	36.0	32.0	36.0
10	34.004308131706566	36.0	36.0	36.0	32.0	36.0
11	34.335963254678816	36.0	36.0	36.0	32.0	36.0
12	34.116056595932946	36.0	36.0	36.0	32.0	36.0
13	34.20976253931206	36.0	36.0	36.0	32.0	36.0
14	34.12413734197199	36.0	36.0	36.0	32.0	36.0
15	34.085297936722576	36.0	36.0	36.0	32.0	36.0
16	34.05359876312761	36.0	36.0	36.0	32.0	36.0
17	33.972869039130195	36.0	36.0	36.0	32.0	36.0
18	33.99166301177658	36.0	36.0	36.0	32.0	36.0
19	33.98329819200505	36.0	36.0	36.0	32.0	36.0
20	33.95806169582421	36.0	36.0	36.0	32.0	36.0
21	33.87796058093078	36.0	36.0	36.0	32.0	36.0
22	33.836978146476476	36.0	36.0	36.0	32.0	36.0
23	33.7462981641735	36.0	36.0	36.0	32.0	36.0
24	33.730182738106095	36.0	36.0	36.0	27.0	36.0
25	33.177975072529975	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	47.0
5	93.0
6	182.0
7	44.0
8	169.0
9	195.0
10	138.0
11	38.0
12	86.0
13	55.0
14	235.0
15	292.0
16	405.0
17	563.0
18	798.0
19	1123.0
20	1707.0
21	2482.0
22	3686.0
23	5149.0
24	6939.0
25	9352.0
26	12457.0
27	16130.0
28	21903.0
29	28926.0
30	37899.0
31	52334.0
32	75021.0
33	107041.0
34	232947.0
35	423538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.75077120353823	16.737084380320923	11.39615194869265	25.115992467448205
2	15.410764981275204	20.775308224897163	36.81855088710033	26.995375906727297
3	19.838010440350928	25.07594160390587	27.842567416469034	27.243480539274167
4	12.522964575522499	15.6838692214217	35.16953951826704	36.62362668478876
5	13.647570257836081	37.43509934792126	33.94006482384657	14.977265570396087
6	34.276090655257306	34.089817990482814	17.555238486621015	14.078852867638869
7	29.324273709483794	30.984201680672268	21.28691476590636	18.404609843937575
8	28.982380369606858	32.222540293064924	20.41483268939998	18.38024664792824
9	27.25160097835965	15.174853928137063	18.295056516985838	39.27848857651745
10	15.44919662255722	27.598688920300308	32.50428330237568	24.44783115476679
11	36.75788490531772	21.871333565871137	20.923405698713644	20.447375830097503
12	23.340867636516	24.624486100940445	29.304979751560197	22.729666510983364
13	30.512953313109747	19.950437158102392	24.968122386972603	24.56848714181526
14	24.007064801682024	19.716004966117396	24.933118852411756	31.34381137978883
15	25.85010809512371	26.121546961325965	24.71631035311074	23.312034590439588
16	25.95066907386572	25.826625940292114	24.929691275761048	23.293013710081123
17	23.73710681429008	25.753988311331398	26.96917166506833	23.539733209310192
18	25.32763261709196	23.94118229318326	28.304171459708176	22.427013630016596
19	24.336316353949236	28.119197813476642	25.325210752712973	22.219275079861152
20	23.93464550964145	24.046881651408768	28.521048600747793	23.49742423820199
21	25.125025946968243	26.00933706457143	23.890719057759874	24.974917930700453
22	23.506834673097792	26.47735344694177	25.177991785512088	24.837820094448347
23	22.79575080323582	25.68067207507311	25.232320882977938	26.291256238713128
24	22.788862310783163	27.978864799877755	25.158211273195914	24.074061616143172
25	25.01256499924562	25.264249381840475	24.345827219007695	25.377358399906207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	195.0
1	195.0
2	490.5
3	786.0
4	786.0
5	786.0
6	2405.5
7	4025.0
8	4025.0
9	4025.0
10	4045.0
11	4065.0
12	4065.0
13	4065.0
14	3719.5
15	3374.0
16	3374.0
17	3374.0
18	5443.5
19	7513.0
20	7513.0
21	7513.0
22	11912.0
23	16311.0
24	16311.0
25	16311.0
26	23911.0
27	31511.0
28	31511.0
29	31511.0
30	38611.0
31	45711.0
32	45711.0
33	45711.0
34	54105.0
35	62499.0
36	62499.0
37	62499.0
38	70518.5
39	78538.0
40	78538.0
41	78538.0
42	94842.0
43	111146.0
44	111146.0
45	111146.0
46	151137.0
47	191128.0
48	191128.0
49	191128.0
50	165702.0
51	140276.0
52	140276.0
53	140276.0
54	122237.0
55	104198.0
56	104198.0
57	104198.0
58	94536.5
59	84875.0
60	84875.0
61	84875.0
62	75220.5
63	65566.0
64	65566.0
65	65566.0
66	54513.5
67	43461.0
68	43461.0
69	43461.0
70	32608.0
71	21755.0
72	21755.0
73	21755.0
74	16881.5
75	12008.0
76	12008.0
77	12008.0
78	9745.0
79	7482.0
80	7482.0
81	7482.0
82	5193.5
83	2905.0
84	2905.0
85	2905.0
86	2184.5
87	1464.0
88	1464.0
89	1464.0
90	1002.5
91	541.0
92	541.0
93	541.0
94	325.0
95	109.0
96	109.0
97	109.0
98	325.0
99	541.0
100	541.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010076939834910934
2	0.004702571922958436
3	0.006142134756517141
4	0.015451307746863431
5	0.0375246045280969
6	0.04788945692971958
7	0.07034663713323538
8	0.09453129273702163
9	0.1014411943381034
10	0.12610570421974254
11	0.12399434539718979
12	0.13522293549894768
13	0.12322657855262514
14	0.12773720876444242
15	0.11833206491852553
16	0.11670056037382567
17	0.12639361678645428
18	0.1355108480656594
19	0.13695041089921814
20	0.12668152935316604
21	0.13426322694324186
22	0.1560486111577636
23	0.1443401667781528
24	0.13858191544391799
25	0.13378337266538898
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1041983.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.14535685024541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.13851778280485	39.68429214818242
2	13.203725163154905	13.242110201179353
3	3.623025348651691	5.450336969568716
4	1.3942335668537869	2.796573589698945
5	0.6841220179810701	1.7152771360385386
6	0.40507386410669416	1.21875440798028
7	0.2365535345282712	0.8303442982174905
8	0.18306117265329946	0.7343734262499271
9	0.1353177766824956	0.6107002379933004
>10	0.7735929535797795	7.586148462985412
>50	0.1022050901183008	3.621316911945037
>100	0.10602656804609376	10.266106837911307
>500	0.009186417371842592	3.303864491512407
>1k	0.004593208685921296	5.048023942762813
>5k	3.827673904934413E-4	1.235134375349
>10k+	3.827673904934413E-4	2.656642562425099
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	15008	1.4403306004032697	No Hit
TCCATGTACTCTGCGTTGATACCAC	12674	1.216334623501535	No Hit
GAGTACATGGAAGCAGTGGTATCAA	6967	0.6686289507602331	No Hit
CATGTACTCTGCGTTGATACCACTG	5903	0.5665159604331357	No Hit
GCTTCCATGTACTCTGCGTTGATAC	4051	0.3887779359164209	No Hit
CCCATGTACTCTGCGTTGATACCAC	3928	0.3769735206812395	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3668	0.35202109823288863	No Hit
GCGTTGATACCACTGCTTCCATGTA	3401	0.32639687979554366	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3356	0.3220781912948676	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3137	0.3010605739249105	No Hit
GTATCAACGCAGAGTACATGGAAGC	2592	0.24875645763894422	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2567	0.2463571862496797	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2465	0.2365681589814805	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2330	0.22361209347945216	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2295	0.22025311353448185	No Hit
CAGTGGTATCAACGCAGAGTACATG	2276	0.21842966727864083	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2031	0.19491680766384864	No Hit
TATCAACGCAGAGTACATGGAAGCA	1665	0.15979147452501624	No Hit
GTATCAACGCAGAGTACATGGGAAG	1621	0.1555687568799107	No Hit
GTACATGGGGTGGTATCAACGCAAA	1526	0.1464515256007056	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1509	0.14482002105600572	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1290	0.12380240368604861	No Hit
ATACCACTGCTTCCATGTACTCTGC	1252	0.12015551117436657	No Hit
GGTATCAACGCAGAGTACATGGAAG	1210	0.1161247352404022	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1168	0.11209395930643783	No Hit
GCGTTGATACCACTGCTTCCCATGT	1125	0.10796721251690286	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1119	0.10739138738347939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9194171114116066E-4
2	0.0	0.0	0.0	0.0	1.9194171114116066E-4
3	0.0	0.0	0.0	0.0	1.9194171114116066E-4
4	0.0	0.0	0.0	9.597085557058033E-5	1.9194171114116066E-4
5	0.0	0.0	0.0	9.597085557058033E-5	1.9194171114116066E-4
6	0.0	0.0	0.0	9.597085557058033E-5	1.9194171114116066E-4
7	0.0	0.0	0.0	1.9194171114116066E-4	1.9194171114116066E-4
8	0.0	0.0	0.0	1.9194171114116066E-4	1.9194171114116066E-4
9	0.0	0.0	0.0	1.9194171114116066E-4	1.9194171114116066E-4
10	9.597085557058033E-5	0.0	0.0	3.838834222823213E-4	1.9194171114116066E-4
11	9.597085557058033E-5	0.0	0.0	3.838834222823213E-4	1.9194171114116066E-4
12	9.597085557058033E-5	0.0	0.0	3.838834222823213E-4	4.7985427785290165E-4
13	9.597085557058033E-5	0.0	0.0	3.838834222823213E-4	6.717959889940623E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	35	0.0021786233	16.275543	1
GCGTTAT	65	1.9251274E-7	16.066883	1
TTAGGAC	125	0.0	15.193427	3
CCCTTAT	45	6.791764E-4	14.768549	1
CGTTATT	65	3.3897595E-6	14.606959	2
TAGGACG	145	0.0	14.411022	4
CAACGGA	60	2.5643238E-5	14.251369	14
ACAGTCG	40	0.005275866	14.250686	8
AAAGGCG	40	0.0052809166	14.248631	5
TCGCCAG	140	0.0	13.575997	17
CGAGGTT	85	2.7024362E-7	13.409187	4
TTCGAAG	50	0.0015054946	13.292333	2
AACGGAC	65	5.4417356E-5	13.155744	15
TTAGGCA	95	7.396011E-8	12.9975	4
GTTATTC	95	7.419294E-8	12.994378	3
CGCCAGT	165	0.0	12.671539	18
TACCTAA	60	4.0784082E-4	12.67093	19
TTTAGGA	160	0.0	12.461562	2
CTTCCGT	100	1.4379293E-7	12.351782	15
GGACGTG	155	1.8189894E-12	12.258654	6
>>END_MODULE