FastQCFastQC Report
Thu 2 Feb 2017
SRR4062545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041983
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA60980.5852302772693988No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC56460.5418514505514965No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA26970.25883339747385514No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC23410.22466777289072856No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15010.14405225421144108No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG14970.14366837078915873No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC14470.13886982801062975No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG14040.13474308122109477No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14010.13445516865438303No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA13440.12898482988685994No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11600.11132619246187318No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11110.10662362053891475No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10510.10086536920467992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG300.00574480729.3331765
GATTTCG701.1320735E-625.14272141
CTATGCG1950.024.82037738
ATTTCGT651.813156E-523.69217742
ATGCGTT1800.023.22209740
GATCTAG500.00257740822.0041051
TATGCGG602.872515E-421.99988239
GTTTCGG500.002580282721.9998821
TATGCGT1750.021.37131139
TGGTATA4300.020.9766344
CGGCTAA1057.7439836E-820.9522679
ACCGTGT755.4719356E-520.5332228
GGTTTCG550.00448334619.99989120
CGGTCCA2800.019.64275210
ATCACGG1252.2871973E-819.35989435
TAAGGGG808.987432E-519.2498954
TAGGACG1152.0208427E-719.1303314
CCTTATA708.119971E-418.8579442
GTCTTAG1658.185452E-1118.6701471
AATCACG1203.1611125E-718.33323534
Produced by FastQC (version 0.11.5)