##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062545_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041983
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.174404956702748	32.0	32.0	32.0	32.0	32.0
2	31.414777400399046	32.0	32.0	32.0	32.0	32.0
3	31.509981448833617	32.0	32.0	32.0	32.0	32.0
4	31.627555343993137	32.0	32.0	32.0	32.0	32.0
5	31.560415093144513	32.0	32.0	32.0	32.0	32.0
6	35.167175472152614	36.0	36.0	36.0	36.0	36.0
7	35.1806459414405	36.0	36.0	36.0	36.0	36.0
8	35.14071150872903	36.0	36.0	36.0	36.0	36.0
9	35.235740890206465	36.0	36.0	36.0	36.0	36.0
10	35.134557857469844	36.0	36.0	36.0	36.0	36.0
11	35.24625641685133	36.0	36.0	36.0	36.0	36.0
12	35.159917196345816	36.0	36.0	36.0	36.0	36.0
13	35.19075647107486	36.0	36.0	36.0	36.0	36.0
14	35.139679822031646	36.0	36.0	36.0	36.0	36.0
15	35.117284063175696	36.0	36.0	36.0	36.0	36.0
16	35.1136851560918	36.0	36.0	36.0	36.0	36.0
17	35.09105714776537	36.0	36.0	36.0	36.0	36.0
18	35.09869258903456	36.0	36.0	36.0	36.0	36.0
19	35.06924297229418	36.0	36.0	36.0	36.0	36.0
20	35.048477758274366	36.0	36.0	36.0	36.0	36.0
21	35.00595115275393	36.0	36.0	36.0	36.0	36.0
22	34.976313433136625	36.0	36.0	36.0	36.0	36.0
23	34.90359919499647	36.0	36.0	36.0	32.0	36.0
24	34.87091919925757	36.0	36.0	36.0	32.0	36.0
25	34.82728221093818	36.0	36.0	36.0	32.0	36.0
26	34.77191086610818	36.0	36.0	36.0	32.0	36.0
27	34.72345326171348	36.0	36.0	36.0	32.0	36.0
28	34.677787449507335	36.0	36.0	36.0	32.0	36.0
29	34.63674839224824	36.0	36.0	36.0	32.0	36.0
30	34.60930648580639	36.0	36.0	36.0	32.0	36.0
31	34.58632626444002	36.0	36.0	36.0	32.0	36.0
32	34.5319539762165	36.0	36.0	36.0	32.0	36.0
33	34.4746305841842	36.0	36.0	36.0	32.0	36.0
34	34.43572879787866	36.0	36.0	36.0	32.0	36.0
35	34.338946028869955	36.0	36.0	36.0	32.0	36.0
36	34.304948353284075	36.0	36.0	36.0	32.0	36.0
37	34.22349596874421	36.0	36.0	36.0	32.0	36.0
38	34.139145264366114	36.0	36.0	36.0	32.0	36.0
39	33.98071081773887	36.0	36.0	36.0	32.0	36.0
40	33.86571182063431	36.0	36.0	36.0	32.0	36.0
41	33.87595862888358	36.0	36.0	36.0	32.0	36.0
42	33.672407323344046	36.0	36.0	36.0	27.0	36.0
43	33.72978733818114	36.0	36.0	36.0	27.0	36.0
44	33.708460694656246	36.0	36.0	36.0	27.0	36.0
45	33.4775749700331	36.0	36.0	36.0	21.0	36.0
46	33.63197096305794	36.0	36.0	36.0	27.0	36.0
47	33.48439082019572	36.0	36.0	36.0	21.0	36.0
48	33.51816584339668	36.0	36.0	36.0	21.0	36.0
49	33.5633901896672	36.0	36.0	36.0	21.0	36.0
50	32.9507343210014	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	34.0
22	135.0
23	390.0
24	1117.0
25	2564.0
26	5799.0
27	11068.0
28	17911.0
29	24727.0
30	32713.0
31	43688.0
32	62608.0
33	81996.0
34	167904.0
35	589319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.105006867993424	17.421665999545013	11.892715319512877	25.58061181294869
2	14.87486311578701	21.18674893635808	36.48774064508575	27.450647302769166
3	19.279234838422383	25.132344698918207	28.299980037927934	27.28844042473147
4	12.244741271922162	16.150087045649542	35.22546454737222	36.37970713505608
5	13.43140914966943	37.50262720217125	33.940764868524724	15.1251987796346
6	34.58211209833605	34.32385328672966	17.08673311720593	14.007301497728358
7	29.06626896267782	31.020815158817676	21.293574450973672	18.619341427530827
8	28.811506521699492	32.382869970047494	20.384689577469114	18.4209339307839
9	27.170884745720418	14.740643561363283	18.364695009419542	39.72377668349675
10	15.389214603309268	27.58989350114157	32.7263496621346	24.294542233414557
11	36.92449876821407	21.571849060877195	21.05226284881807	20.45138932209067
12	23.31563950659464	24.6111500859419	29.34692792492776	22.726282482535705
13	30.738505330701173	19.76327828764961	25.043402819431794	24.454813562217424
14	24.021505149316255	19.493216300073993	25.065955970490883	31.41932258011887
15	25.951766967407337	26.078448496760505	24.65855968859377	23.31122484723839
16	26.052344424045305	25.568843253680722	25.026895832273656	23.351916490000317
17	23.727066564425712	25.62450634991166	27.123763055635266	23.524664030027363
18	25.524216805840403	23.552111694720548	28.460349161166736	22.463322338272313
19	24.466330064885895	27.677610863133083	25.58237514431617	22.273683927664848
20	23.971888216986265	23.820254265184747	28.769567257815147	23.43829026001384
21	25.215286621758704	25.811361605707578	24.17285118855106	24.800500583982657
22	23.638485464734067	26.076433108793523	25.47421599008813	24.81086543638428
23	22.811792514849092	25.105783875552675	25.685735755765688	26.396687853832546
24	22.92753336666721	27.585766754352036	25.26778267975581	24.21891719922494
25	25.180449201186583	24.625065859999633	24.733800839361102	25.460684099452678
26	22.86668784423546	25.086397762727415	26.286993165915373	25.759921227121747
27	25.58535024083886	25.81923121586437	25.69379730763362	22.901621235663153
28	24.124289935632348	23.806530432838155	26.346303154657996	25.722876476871505
29	22.989626510221374	26.14946692988273	27.537013559722183	23.323893000173708
30	25.314232573851974	24.783321800835523	26.837481993468227	23.064963631844282
31	23.14087657860061	25.434963910159762	25.8249894671986	25.599170044041024
32	23.087132899481087	25.963475411786945	26.22384434294993	24.725547345782033
33	23.132143230743687	25.167972989962408	26.340448932468185	25.359434846825717
34	22.81620717420534	26.410219744468	27.391617713532757	23.381955367793907
35	25.15856784611649	25.460588128597106	26.295438601205586	23.085405424080815
36	23.95941200576209	26.244094193475327	26.03219054437548	23.7643032563871
37	25.154441099326956	25.762704381933293	26.30474777419593	22.778106744543816
38	24.068051014267986	25.65656061567223	25.28851238455906	24.986875985500724
39	23.656432014725766	25.60953489644265	26.49477006822568	24.239263020605904
40	24.795030245215134	25.807522771484752	25.362409943348403	24.035037039951707
41	22.694995983619695	25.843991696601577	26.765215939223573	24.69579638055515
42	25.832475193933107	26.04188360078811	25.756082392898925	22.369558812379857
43	24.46863336541959	24.244637388517855	25.45300643100703	25.833722815055527
44	24.006917579269526	24.99081079057912	27.46206032152156	23.540211308629797
45	24.704529728412076	25.306458934550758	26.031038894108637	23.957972442928533
46	23.480517436464897	25.021425493506133	27.307355302341783	24.19070176768719
47	24.408195151163838	24.48142098423965	27.66621688282523	23.44416698177128
48	23.90461264723129	28.049018074191228	24.704529728412076	23.341839550165407
49	22.779450336521805	25.41586570990122	28.21475974176162	23.589924211815354
50	22.596146002381996	27.996905899616404	24.39655925288608	25.010388845115518
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	16.0
2	18.0
3	88.0
4	158.0
5	182.5
6	207.0
7	213.0
8	219.0
9	294.0
10	369.0
11	613.0
12	857.0
13	1414.5
14	1972.0
15	2810.0
16	3648.0
17	4554.5
18	5461.0
19	5881.5
20	6302.0
21	7038.5
22	7775.0
23	7927.0
24	8079.0
25	8572.5
26	9066.0
27	10812.5
28	12559.0
29	13954.0
30	15349.0
31	16404.0
32	17459.0
33	20039.5
34	22620.0
35	25506.5
36	28393.0
37	33424.5
38	38456.0
39	40985.5
40	43515.0
41	48477.0
42	53439.0
43	57331.0
44	61223.0
45	72445.0
46	83667.0
47	92501.0
48	101335.0
49	96304.5
50	91274.0
51	80678.5
52	70083.0
53	65651.5
54	61220.0
55	61969.0
56	62718.0
57	60309.0
58	57900.0
59	53182.0
60	48464.0
61	42387.0
62	36310.0
63	31373.0
64	26436.0
65	22785.0
66	19134.0
67	16770.5
68	14407.0
69	13076.5
70	11746.0
71	9288.0
72	6830.0
73	6078.0
74	5326.0
75	3933.0
76	2540.0
77	2302.0
78	2064.0
79	1693.0
80	1322.0
81	1078.0
82	834.0
83	704.5
84	575.0
85	477.5
86	380.0
87	282.0
88	184.0
89	112.5
90	41.0
91	27.0
92	13.0
93	13.0
94	13.0
95	9.0
96	5.0
97	3.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018618345980692584
2	0.004222717645105535
3	6.717959889940623E-4
4	9.597085557058033E-5
5	0.0
6	4.798542778529016E-4
7	1.9194171114116066E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	9.597085557058033E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1041983.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.81868185858904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87648152191304	44.5859991005513
2	12.700331334740108	14.178315085450555
3	3.5144619522738814	5.885179008542747
4	1.3581954829440488	3.0325072625690623
5	0.6609093817404439	1.844554525836331
6	0.3954026587579083	1.3242513129148754
7	0.2478329131631978	0.9683594573760702
8	0.1641662925118958	0.7330836842900463
9	0.12953696150189367	0.6507524198702249
>10	0.7610402061269032	8.291622248562499
>50	0.09306123319577565	3.6433118734613523
>100	0.08958679279009414	9.567009735239285
>500	0.006744951255668889	2.5246355454894993
>1k	0.001902422149034815	1.6366882471026678
>5k	3.458949361881482E-4	1.1337304927435254
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6098	0.5852302772693988	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5646	0.5418514505514965	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2697	0.25883339747385514	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2341	0.22466777289072856	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1501	0.14405225421144108	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1497	0.14366837078915873	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1447	0.13886982801062975	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1404	0.13474308122109477	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1401	0.13445516865438303	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1344	0.12898482988685994	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1160	0.11132619246187318	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1111	0.10662362053891475	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1051	0.10086536920467992	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.87912566711741E-4
2	0.0	0.0	0.0	0.0	2.87912566711741E-4
3	0.0	0.0	0.0	0.0	2.87912566711741E-4
4	0.0	0.0	0.0	0.0	2.87912566711741E-4
5	0.0	0.0	0.0	0.0	3.838834222823213E-4
6	0.0	0.0	0.0	0.0	4.7985427785290165E-4
7	0.0	0.0	0.0	0.0	4.7985427785290165E-4
8	0.0	0.0	0.0	0.0	4.7985427785290165E-4
9	0.0	0.0	0.0	9.597085557058033E-5	4.7985427785290165E-4
10	0.0	0.0	0.0	9.597085557058033E-5	4.7985427785290165E-4
11	0.0	0.0	0.0	9.597085557058033E-5	4.7985427785290165E-4
12	0.0	0.0	0.0	9.597085557058033E-5	5.75825133423482E-4
13	0.0	0.0	0.0	9.597085557058033E-5	5.75825133423482E-4
14	0.0	0.0	0.0	1.9194171114116066E-4	5.75825133423482E-4
15	0.0	0.0	0.0	2.87912566711741E-4	8.63737700135223E-4
16	0.0	0.0	0.0	2.87912566711741E-4	8.63737700135223E-4
17	0.0	0.0	0.0	4.7985427785290165E-4	8.63737700135223E-4
18	0.0	0.0	0.0	0.0010556794112763835	8.63737700135223E-4
19	0.0	0.0	0.0	0.0014395628335587048	8.63737700135223E-4
20	0.0	0.0	0.0	0.002015387966982187	0.0010556794112763835
21	0.0	0.0	0.0	0.0026871839559762492	0.001151650266846964
22	0.0	0.0	0.0	0.0037428633672526327	0.001151650266846964
23	0.0	0.0	0.0	0.006334076467658302	0.001151650266846964
24	0.0	0.0	0.0	0.010268881546052095	0.001151650266846964
25	0.0	0.0	0.0	0.013819803202163567	0.001151650266846964
26	0.0	0.0	0.0	0.01794654999169852	0.0014395628335587048
27	0.0	0.0	0.0	0.024184655603786243	0.0015355336891292853
28	0.0	0.0	0.0	0.034069653727556014	0.0015355336891292853
29	0.0	0.0	0.0	0.05124843687468989	0.0015355336891292853
30	0.0	0.0	0.0	0.07792833472331122	0.0015355336891292853
31	0.0	0.0	0.0	0.1292727424535717	0.0015355336891292853
32	0.0	0.0	0.0	0.19395709910814285	0.0015355336891292853
33	0.0	0.0	0.0	0.25297917528404973	0.0015355336891292853
34	0.0	9.597085557058033E-5	0.0	0.3303316848739375	0.0015355336891292853
35	0.0	9.597085557058033E-5	0.0	0.40566880649684306	0.0015355336891292853
36	0.0	9.597085557058033E-5	0.0	0.5221774251595276	0.0015355336891292853
37	0.0	9.597085557058033E-5	0.0	0.7064414678550418	0.0015355336891292853
38	0.0	9.597085557058033E-5	0.0	0.8937765779288146	0.0016315045446998655
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAACCG	30	0.005744807	29.333176	5
GATTTCG	70	1.1320735E-6	25.142721	41
CTATGCG	195	0.0	24.820377	38
ATTTCGT	65	1.813156E-5	23.692177	42
ATGCGTT	180	0.0	23.222097	40
GATCTAG	50	0.002577408	22.004105	1
TATGCGG	60	2.872515E-4	21.999882	39
GTTTCGG	50	0.0025802827	21.99988	21
TATGCGT	175	0.0	21.371311	39
TGGTATA	430	0.0	20.97663	44
CGGCTAA	105	7.7439836E-8	20.952267	9
ACCGTGT	75	5.4719356E-5	20.533222	8
GGTTTCG	55	0.004483346	19.999891	20
CGGTCCA	280	0.0	19.642752	10
ATCACGG	125	2.2871973E-8	19.359894	35
TAAGGGG	80	8.987432E-5	19.249895	4
TAGGACG	115	2.0208427E-7	19.130331	4
CCTTATA	70	8.119971E-4	18.857944	2
GTCTTAG	165	8.185452E-11	18.670147	1
AATCACG	120	3.1611125E-7	18.333235	34
>>END_MODULE