FastQCFastQC Report
Thu 2 Feb 2017
SRR4062544_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062544_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686845
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG67390.9811529529952172No Hit
TCCATGTACTCTGCGTTGATACCAC51930.7560657790331152No Hit
GAGTACATGGAAGCAGTGGTATCAA28550.4156687462236749No Hit
GTACATGGGAAGCAGTGGTATCAAC26100.37999839847418265No Hit
CATGTACTCTGCGTTGATACCACTG25720.3744658547416084No Hit
CCCATGTACTCTGCGTTGATACCAC24210.35248127306743154No Hit
GTATCAACGCAGAGTACTTTTTTTT20270.29511753015600317No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19560.28478040897145646No Hit
GCTTCCATGTACTCTGCGTTGATAC17860.26002955543099243No Hit
TATCAACGCAGAGTACTTTTTTTTT15840.2306197176946764No Hit
CATGGAAGCAGTGGTATCAACGCAG15130.22028259651012966No Hit
GCGTTGATACCACTGCTTCCATGTA13910.20252021926344374No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13670.1990259811165547No Hit
GTATCAACGCAGAGTACATGGAAGC11350.16524834569662733No Hit
ACTCTGCGTTGATACCACTGCTTCC11270.1640835996476643No Hit
GTACATGGGGTGGTATCAACGCAAA9800.14268139099796898No Hit
ACGCAGAGTACATGGAAGCAGTGGT9610.13991511913168184No Hit
CAGTGGTATCAACGCAGAGTACATG9590.1396239326194411No Hit
GTATCAACGCAGAGTACATGGGAAG9370.1364208809847928No Hit
GAGTACATGGGAAGCAGTGGTATCA9090.13234426981342226No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9040.13161630353282036No Hit
GGTATCAACGCAGAGTACTTTTTTT7810.11370833303001404No Hit
GCGTTGATACCACTGCTTCCCATGT7330.10671985673623596No Hit
TATCAACGCAGAGTACATGGAAGCA7240.10540951743115258No Hit
GCTTCCCATGTACTCTGCGTTGATA7120.10366239835770806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTC607.718154E-817.4064483
CGTTATT508.746313E-515.1910832
GCGTTAT508.7626366E-515.1877611
GTCCTAT1300.014.6036171
ACATTAC400.005295709714.2416383
GTCTAAA400.00530339514.2385261
ATTACCG1101.8553692E-1013.81913715
TATTCCC852.6941052E-713.4107355
GATATAC2050.012.9651621
TCCATTA1102.732122E-912.95544058
ACGGTAT751.47358005E-512.6684669
CTCCCCG604.089494E-412.6656955
ACAATAC1207.4396667E-1012.6592353
CCTTTAG905.443144E-712.6564681
CCGGTCC1457.2759576E-1212.4500449
CCTATTC1155.347829E-912.3840343
CGACCAT853.9280058E-612.29586310
CGGTCCA1405.2750693E-1112.21602110
AAACTGT701.0884851E-412.215136
CGGAACC701.0884851E-412.2151314
Produced by FastQC (version 0.11.5)