##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062544_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686845
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95299812912666	32.0	32.0	32.0	32.0	32.0
2	30.582422526188587	32.0	32.0	32.0	32.0	32.0
3	30.58492964205898	32.0	32.0	32.0	32.0	32.0
4	30.647236276015693	32.0	32.0	32.0	32.0	32.0
5	30.413320327002452	32.0	32.0	32.0	32.0	32.0
6	34.02697843035911	36.0	36.0	36.0	32.0	36.0
7	33.857797610814664	36.0	36.0	36.0	32.0	36.0
8	33.83801003137535	36.0	36.0	36.0	32.0	36.0
9	34.01977302011371	36.0	36.0	36.0	32.0	36.0
10	33.65437616929584	36.0	36.0	36.0	27.0	36.0
11	34.081878735376975	36.0	36.0	36.0	32.0	36.0
12	33.79036755017508	36.0	36.0	36.0	32.0	36.0
13	33.934503417801686	36.0	36.0	36.0	32.0	36.0
14	33.81651027524405	36.0	36.0	36.0	32.0	36.0
15	33.76789523109289	36.0	36.0	36.0	32.0	36.0
16	33.753538280106866	36.0	36.0	36.0	32.0	36.0
17	33.64581819770108	36.0	36.0	36.0	27.0	36.0
18	33.67335570616369	36.0	36.0	36.0	27.0	36.0
19	33.665423785570255	36.0	36.0	36.0	27.0	36.0
20	33.62367055158005	36.0	36.0	36.0	27.0	36.0
21	33.5833339399719	36.0	36.0	36.0	27.0	36.0
22	33.518793905466296	36.0	36.0	36.0	27.0	36.0
23	33.44847381869272	36.0	36.0	36.0	27.0	36.0
24	33.411956118192606	36.0	36.0	36.0	27.0	36.0
25	32.86408723947907	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	25.0
5	60.0
6	119.0
7	36.0
8	103.0
9	114.0
10	67.0
11	32.0
12	59.0
13	40.0
14	184.0
15	254.0
16	327.0
17	514.0
18	690.0
19	979.0
20	1451.0
21	2028.0
22	3057.0
23	4446.0
24	5773.0
25	7916.0
26	10379.0
27	13487.0
28	17379.0
29	22592.0
30	28402.0
31	38547.0
32	53559.0
33	73915.0
34	150555.0
35	249751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.27094821171938	16.77531425775761	11.056427341043745	24.897310189479263
2	15.380135435579284	20.816631989994484	37.97292162004365	25.830310954382586
3	19.65498614615623	25.730002198560598	27.94967480231155	26.66533685297162
4	11.9968663814569	15.892484266380874	36.020013221773404	36.09063613038882
5	13.421243116573676	37.93752812719829	34.05921530760414	14.582013448623899
6	33.28094457084427	35.012686917181085	18.022851016846943	13.6835174951277
7	28.894923709219434	30.995050896942978	21.63327685401967	18.476748539817915
8	28.150250860123077	33.011385246248025	20.479453951174445	18.358909942454453
9	27.303887064831045	14.514156971957116	18.42074966591324	39.76120629729859
10	15.09357503421877	27.91070905372529	32.78194590008848	24.21377001196746
11	36.614505513727835	21.615708111338673	21.822700669810427	19.947085705123065
12	23.341570265404158	24.523460677761015	30.12894807817067	22.00602097866416
13	30.17853863040696	20.048133128478426	25.3943797065366	24.37894853457801
14	23.443918680556163	20.052304274431258	25.796299049834836	30.707477995177747
15	25.50592357653424	26.85359554099278	24.3413227766182	23.29915810585478
16	25.146889270779127	26.035002718417168	25.59626622122523	23.22184178957848
17	23.414937069787374	26.06144624328351	26.97252761669859	23.551089070230525
18	24.579910952493417	24.766811580257926	28.20143863915349	22.45183882809516
19	24.162659894273695	27.425275760217634	25.791124938404074	22.620939407104597
20	23.97037322103029	24.6482147985207	27.85520720815276	23.52620477229625
21	25.152234793650237	25.728664167931342	24.634410530259625	24.484690508158792
22	23.869641294838146	26.15135608048994	25.45158938466025	24.527413240011665
23	22.863695545359246	25.70607506681305	25.480084389293	25.950144998534707
24	22.778948964447437	27.681750190257087	25.53235536816569	24.006945477129786
25	24.50765003025024	25.37535808264511	25.070085794050545	25.046906093054105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	215.0
1	215.0
2	465.5
3	716.0
4	716.0
5	716.0
6	1794.5
7	2873.0
8	2873.0
9	2873.0
10	2832.0
11	2791.0
12	2791.0
13	2791.0
14	2691.0
15	2591.0
16	2591.0
17	2591.0
18	4087.5
19	5584.0
20	5584.0
21	5584.0
22	8532.5
23	11481.0
24	11481.0
25	11481.0
26	16956.5
27	22432.0
28	22432.0
29	22432.0
30	28224.0
31	34016.0
32	34016.0
33	34016.0
34	39260.5
35	44505.0
36	44505.0
37	44505.0
38	50009.0
39	55513.0
40	55513.0
41	55513.0
42	64905.5
43	74298.0
44	74298.0
45	74298.0
46	94709.0
47	115120.0
48	115120.0
49	115120.0
50	104131.5
51	93143.0
52	93143.0
53	93143.0
54	80624.0
55	68105.0
56	68105.0
57	68105.0
58	61433.5
59	54762.0
60	54762.0
61	54762.0
62	48497.5
63	42233.0
64	42233.0
65	42233.0
66	34642.0
67	27051.0
68	27051.0
69	27051.0
70	20311.0
71	13571.0
72	13571.0
73	13571.0
74	10675.0
75	7779.0
76	7779.0
77	7779.0
78	6177.5
79	4576.0
80	4576.0
81	4576.0
82	3209.0
83	1842.0
84	1842.0
85	1842.0
86	1367.5
87	893.0
88	893.0
89	893.0
90	619.0
91	345.0
92	345.0
93	345.0
94	203.0
95	61.0
96	61.0
97	61.0
98	205.0
99	349.0
100	349.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009900341416185603
2	0.0034942381468890363
3	0.004658984195852048
4	0.014413732355917275
5	0.03377763541992735
6	0.045279502653437095
7	0.06668171130313244
8	0.08822951320894817
9	0.09405324345376322
10	0.11909528350646799
11	0.12084240257991251
12	0.1324898630695426
13	0.12098799583603287
14	0.12506460700740343
15	0.11254358698105105
16	0.11414511279837518
17	0.12331748793395891
18	0.133800202374626
19	0.13438257539910753
20	0.12288070816559778
21	0.1307427439960981
22	0.15214495264579345
23	0.14209901797348748
24	0.13554732144807052
25	0.13103393050833884
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	686845.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.55682716411043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.22357303978785	45.12524009022994
2	11.842818635924576	13.158988561839358
3	3.289651349487984	5.482877743610597
4	1.2494213154446008	2.776555363092448
5	0.6051182204877487	1.680922419474596
6	0.3607147516376589	1.2024100267367062
7	0.2290291394098401	0.8906892619616209
8	0.15487736986769357	0.6883596215497165
9	0.11373552411684852	0.568690637020134
>10	0.7376704348600351	7.765457981299565
>50	0.09918796269932685	3.8955922430071186
>100	0.08370618315322188	8.963414268844437
>500	0.006560045701648991	2.5124092300412273
>1k	0.0034112237648574752	3.548920175010075
>5k	5.248036561319193E-4	1.7394723762824602
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6739	0.9811529529952172	No Hit
TCCATGTACTCTGCGTTGATACCAC	5193	0.7560657790331152	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2855	0.4156687462236749	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2610	0.37999839847418265	No Hit
CATGTACTCTGCGTTGATACCACTG	2572	0.3744658547416084	No Hit
CCCATGTACTCTGCGTTGATACCAC	2421	0.35248127306743154	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2027	0.29511753015600317	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1956	0.28478040897145646	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1786	0.26002955543099243	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1584	0.2306197176946764	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1513	0.22028259651012966	No Hit
GCGTTGATACCACTGCTTCCATGTA	1391	0.20252021926344374	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1367	0.1990259811165547	No Hit
GTATCAACGCAGAGTACATGGAAGC	1135	0.16524834569662733	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1127	0.1640835996476643	No Hit
GTACATGGGGTGGTATCAACGCAAA	980	0.14268139099796898	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	961	0.13991511913168184	No Hit
CAGTGGTATCAACGCAGAGTACATG	959	0.1396239326194411	No Hit
GTATCAACGCAGAGTACATGGGAAG	937	0.1364208809847928	No Hit
GAGTACATGGGAAGCAGTGGTATCA	909	0.13234426981342226	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	904	0.13161630353282036	No Hit
GGTATCAACGCAGAGTACTTTTTTT	781	0.11370833303001404	No Hit
GCGTTGATACCACTGCTTCCCATGT	733	0.10671985673623596	No Hit
TATCAACGCAGAGTACATGGAAGCA	724	0.10540951743115258	No Hit
GCTTCCCATGTACTCTGCGTTGATA	712	0.10366239835770806	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.455932561203765E-4
2	0.0	0.0	0.0	0.0	1.455932561203765E-4
3	0.0	0.0	0.0	0.0	1.455932561203765E-4
4	0.0	0.0	0.0	0.0	1.455932561203765E-4
5	0.0	0.0	0.0	0.0	1.455932561203765E-4
6	0.0	0.0	0.0	0.0	1.455932561203765E-4
7	0.0	0.0	0.0	2.91186512240753E-4	1.455932561203765E-4
8	0.0	0.0	0.0	5.82373024481506E-4	1.455932561203765E-4
9	0.0	0.0	0.0	5.82373024481506E-4	1.455932561203765E-4
10	0.0	0.0	0.0	0.0010191527928426355	1.455932561203765E-4
11	0.0	0.0	0.0	0.001164746048963012	1.455932561203765E-4
12	0.0	0.0	0.0	0.001455932561203765	4.3677976836112954E-4
13	0.0	0.0	0.0	0.001455932561203765	5.82373024481506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATTC	60	7.718154E-8	17.406448	3
CGTTATT	50	8.746313E-5	15.191083	2
GCGTTAT	50	8.7626366E-5	15.187761	1
GTCCTAT	130	0.0	14.603617	1
ACATTAC	40	0.0052957097	14.241638	3
GTCTAAA	40	0.005303395	14.238526	1
ATTACCG	110	1.8553692E-10	13.819137	15
TATTCCC	85	2.6941052E-7	13.410735	5
GATATAC	205	0.0	12.965162	1
TCCATTA	110	2.732122E-9	12.9554405	8
ACGGTAT	75	1.47358005E-5	12.668466	9
CTCCCCG	60	4.089494E-4	12.665695	5
ACAATAC	120	7.4396667E-10	12.659235	3
CCTTTAG	90	5.443144E-7	12.656468	1
CCGGTCC	145	7.2759576E-12	12.450044	9
CCTATTC	115	5.347829E-9	12.384034	3
CGACCAT	85	3.9280058E-6	12.295863	10
CGGTCCA	140	5.2750693E-11	12.216021	10
AAACTGT	70	1.0884851E-4	12.21513	6
CGGAACC	70	1.0884851E-4	12.21513	14
>>END_MODULE