FastQCFastQC Report
Thu 2 Feb 2017
SRR4062544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686845
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24650.35888737633672807No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21500.31302550065880946No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA9710.1413710516928856No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC9240.13452816865522788No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9240.13452816865522788No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7900.11501867233509745No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7850.11429070605449557No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC6900.1004593467230598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATG353.2165195E-431.42841532
ATACGAA1800.025.6665440
CGCAATA2100.025.14273336
GTAATAC551.5926953E-423.999883
TATGCGT752.0621974E-623.4665539
GTAAACG1153.5106495E-1022.95640827
AATACGA2050.022.53647439
TAACGCC2000.021.999894
ATAACGC2050.021.4633083
AACGCTT1451.2732926E-1121.24127430
CGAATGC2200.020.99989543
CAATACG2200.020.99989538
GCGCAAT2550.020.70577835
GCATCGC2150.020.46501514
ATTTAAC654.929348E-420.307593
TACGAAT2300.020.08685741
AACGTAT550.00448180419.999931
CAACGTA550.00448180419.999930
CGCATCG2200.019.999913
CATCGTT2200.019.999928
Produced by FastQC (version 0.11.5)