##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062544_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686845
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11044850002548	32.0	32.0	32.0	32.0	32.0
2	31.331077608485174	32.0	32.0	32.0	32.0	32.0
3	31.458340673659997	32.0	32.0	32.0	32.0	32.0
4	31.5802153324258	32.0	32.0	32.0	32.0	32.0
5	31.510218462680808	32.0	32.0	32.0	32.0	32.0
6	35.08801257925733	36.0	36.0	36.0	36.0	36.0
7	35.106606293996464	36.0	36.0	36.0	36.0	36.0
8	35.05048446155974	36.0	36.0	36.0	36.0	36.0
9	35.156536045250384	36.0	36.0	36.0	36.0	36.0
10	35.049548296922886	36.0	36.0	36.0	36.0	36.0
11	35.183408192532525	36.0	36.0	36.0	36.0	36.0
12	35.08118425554528	36.0	36.0	36.0	36.0	36.0
13	35.12970612001252	36.0	36.0	36.0	36.0	36.0
14	35.08497841579978	36.0	36.0	36.0	36.0	36.0
15	35.04998507669125	36.0	36.0	36.0	36.0	36.0
16	35.05107993797727	36.0	36.0	36.0	36.0	36.0
17	35.02156527309655	36.0	36.0	36.0	36.0	36.0
18	35.033471889582074	36.0	36.0	36.0	36.0	36.0
19	34.984561291121	36.0	36.0	36.0	36.0	36.0
20	34.98372704176342	36.0	36.0	36.0	36.0	36.0
21	34.951264113446264	36.0	36.0	36.0	32.0	36.0
22	34.916886633811124	36.0	36.0	36.0	32.0	36.0
23	34.85049465308767	36.0	36.0	36.0	32.0	36.0
24	34.82328472945133	36.0	36.0	36.0	32.0	36.0
25	34.785423203197226	36.0	36.0	36.0	32.0	36.0
26	34.722477414846146	36.0	36.0	36.0	32.0	36.0
27	34.68394761554645	36.0	36.0	36.0	32.0	36.0
28	34.63758198720235	36.0	36.0	36.0	32.0	36.0
29	34.60472450116111	36.0	36.0	36.0	32.0	36.0
30	34.573276357839106	36.0	36.0	36.0	32.0	36.0
31	34.53626218433562	36.0	36.0	36.0	32.0	36.0
32	34.49412895194695	36.0	36.0	36.0	32.0	36.0
33	34.436117319045785	36.0	36.0	36.0	32.0	36.0
34	34.38555278119517	36.0	36.0	36.0	32.0	36.0
35	34.328127889116175	36.0	36.0	36.0	32.0	36.0
36	34.25431938792595	36.0	36.0	36.0	32.0	36.0
37	34.1887339938414	36.0	36.0	36.0	32.0	36.0
38	34.11506380624449	36.0	36.0	36.0	32.0	36.0
39	33.984096848633975	36.0	36.0	36.0	32.0	36.0
40	33.88459987333387	36.0	36.0	36.0	32.0	36.0
41	33.872851953497516	36.0	36.0	36.0	32.0	36.0
42	33.721206385720215	36.0	36.0	36.0	27.0	36.0
43	33.74639838682672	36.0	36.0	36.0	32.0	36.0
44	33.73494165350261	36.0	36.0	36.0	32.0	36.0
45	33.54706520393975	36.0	36.0	36.0	21.0	36.0
46	33.65041748866192	36.0	36.0	36.0	27.0	36.0
47	33.54126476861592	36.0	36.0	36.0	21.0	36.0
48	33.52381104907221	36.0	36.0	36.0	21.0	36.0
49	33.56082522257569	36.0	36.0	36.0	21.0	36.0
50	32.97342486296035	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	19.0
22	80.0
23	303.0
24	768.0
25	1913.0
26	4032.0
27	7456.0
28	11665.0
29	16570.0
30	21592.0
31	28969.0
32	41915.0
33	57578.0
34	118064.0
35	375916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57907229029698	17.332634383941638	11.586396697464124	25.501896628297256
2	14.839868843293361	21.24220310652697	37.45187911684712	26.46604893333256
3	19.03814851159364	25.77201161256883	28.47365187335661	26.716188002480916
4	11.890909726226043	16.343303574028457	36.11562450862205	35.65016219112346
5	13.261507326980615	37.91947237003982	33.99558852433955	14.823431778640014
6	33.658590561716615	35.152531663077774	17.62503985638598	13.56383791881964
7	28.639836702366182	30.946182830453495	21.74846107704224	18.66551939013808
8	28.084356732596145	33.07500236589041	20.432994343702	18.40764655781144
9	27.209923636337162	14.105511432710438	18.410412829677732	40.274152101274666
10	14.957086387758519	27.871353798892034	32.898397746216396	24.27316206713305
11	36.836695324272576	21.327373716049472	21.834038247348385	20.001892712329564
12	23.196208751610627	24.361391580341998	30.351098137134287	22.09130153091309
13	30.457235620846042	19.611848379183076	25.58830594966841	24.342610050302472
14	23.506031200634787	19.655089576250827	25.85867262628395	30.980206596830435
15	25.596604765267273	26.736891147202062	24.367506497099054	23.29899759043161
16	25.212092975853356	25.88939280332535	25.65003749026345	23.24847673055784
17	23.412851516717744	25.813247530374394	27.18488159628446	23.5890193566234
18	24.79860812847149	24.374931753161196	28.36739002249416	22.45907009587316
19	24.224097139820483	27.108008357052903	26.02741521012747	22.64047929299915
20	24.02172251381316	24.282480035524753	28.169092007658204	23.52670544300388
21	25.288529435316555	25.503425081350233	24.858592549993084	24.349452933340128
22	23.76256651791889	25.744236326973336	25.764182603061826	24.72901455204595
23	22.992523786298218	25.09197853955405	25.91661874221986	25.99887893192787
24	23.056439225735065	27.194490751188404	25.74161564836317	24.00745437471336
25	24.66029453515713	24.721589295983808	25.36627623408484	25.251839934774225
26	22.886677489098705	25.41330285581172	26.97333459514155	24.72668505994802
27	24.895864423559903	25.780489047747306	25.969760280703795	23.353886247988992
28	23.70447480872686	24.709941835494178	26.637450953271845	24.948132402507117
29	23.094147879070242	25.670566139376422	27.305723998864373	23.929561982688963
30	24.34202767727799	25.435869810510376	27.09257547190414	23.129527040307494
31	23.455655934017138	25.42232963769118	26.02610487082238	25.095909557469298
32	23.076385501823555	26.013729444052153	26.253521536882413	24.656363517241882
33	23.21367994234507	25.32551012237113	26.502049225079894	24.958760710203904
34	23.045810918038274	26.265023404115922	27.42918708005445	23.25997859779135
35	24.79598744986132	25.671002919144787	26.577320938494132	22.955688692499763
36	23.678122429369072	26.4192066623474	26.31568985724581	23.586981051037718
37	24.821175083170147	26.016786902430677	26.30709985513471	22.854938159264464
38	24.035408279888475	25.817032955033525	25.665907155180573	24.48165160989743
39	24.00148505121243	25.45610727311111	26.3341802007731	24.208227474903364
40	24.850439327650342	25.975729604204734	25.508084065546083	23.665747002598838
41	22.696823883117734	25.96743078860587	27.155471758548146	24.18027356972825
42	25.471685751515988	26.32515341889364	25.987231471438243	22.21592935815213
43	24.18464136741186	24.62476978066376	25.920404166878992	25.27018468504539
44	23.63022224810547	25.680466480792607	26.881028470761237	23.808282800340688
45	24.27621952551158	25.85212092975853	26.095552853991805	23.776106690738086
46	23.232674716055925	25.43798218806918	27.11842444343176	24.210918652443134
47	24.35804293545123	25.077564807198133	27.05661393764241	23.50777831970823
48	24.079668629749072	27.373570456216466	24.89499086402318	23.651770050011283
49	22.949428182486585	25.996840626342188	27.46063522337645	23.59309596779477
50	23.087450589288704	27.594726612263322	24.674125894488565	24.64369690395941
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	20.5
2	20.0
3	110.0
4	200.0
5	221.0
6	242.0
7	225.5
8	209.0
9	276.5
10	344.0
11	493.0
12	642.0
13	1067.0
14	1492.0
15	2055.0
16	2618.0
17	3073.0
18	3528.0
19	3719.5
20	3911.0
21	4433.0
22	4955.0
23	5331.0
24	5707.0
25	5921.0
26	6135.0
27	7595.0
28	9055.0
29	10312.0
30	11569.0
31	12479.5
32	13390.0
33	15191.5
34	16993.0
35	19303.0
36	21613.0
37	24452.5
38	27292.0
39	28955.5
40	30619.0
41	33345.0
42	36071.0
43	37713.0
44	39355.0
45	45713.5
46	52072.0
47	56458.5
48	60845.0
49	59575.0
50	58305.0
51	52626.0
52	46947.0
53	44028.0
54	41109.0
55	40579.5
56	40050.0
57	38798.5
58	37547.0
59	34245.5
60	30944.0
61	27196.5
62	23449.0
63	20418.0
64	17387.0
65	14909.0
66	12431.0
67	10901.5
68	9372.0
69	8580.0
70	7788.0
71	6032.5
72	4277.0
73	3769.0
74	3261.0
75	2405.5
76	1550.0
77	1430.5
78	1311.0
79	1099.0
80	887.0
81	720.0
82	553.0
83	462.5
84	372.0
85	312.0
86	252.0
87	176.0
88	100.0
89	65.0
90	30.0
91	22.5
92	15.0
93	9.5
94	4.0
95	2.5
96	1.0
97	2.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01455932561203765
2	0.004804577451972425
3	4.3677976836112954E-4
4	1.455932561203765E-4
5	0.0
6	5.82373024481506E-4
7	1.455932561203765E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.91186512240753E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	686845.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.93786447772168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.49001566520033	49.65827530893395
2	11.865829889819919	14.461566674830909
3	3.156749054108147	5.770966381482551
4	1.1984966368568597	2.9213530253515416
5	0.5793946466076222	1.7653536227046362
6	0.36785143596618936	1.344964857170578
7	0.21387185639113798	0.9123025940253344
8	0.16121803051631062	0.7859425995974507
9	0.12050618178556698	0.6609050436938917
>10	0.687295743293617	7.972207677949059
>50	0.08730011164982415	3.734584009626303
>100	0.0669138880452525	7.673760258302541
>500	0.004077190310897649	1.6633349616902482
>1k	4.796694483408999E-4	0.674482984641112
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2465	0.35888737633672807	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2150	0.31302550065880946	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	971	0.1413710516928856	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	924	0.13452816865522788	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	924	0.13452816865522788	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	790	0.11501867233509745	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	785	0.11429070605449557	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	690	0.1004593467230598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.455932561203765E-4
2	0.0	0.0	0.0	0.0	1.455932561203765E-4
3	0.0	0.0	0.0	0.0	1.455932561203765E-4
4	0.0	0.0	0.0	0.0	1.455932561203765E-4
5	0.0	0.0	0.0	0.0	1.455932561203765E-4
6	0.0	0.0	0.0	1.455932561203765E-4	1.455932561203765E-4
7	0.0	0.0	0.0	4.3677976836112954E-4	1.455932561203765E-4
8	0.0	0.0	0.0	5.82373024481506E-4	1.455932561203765E-4
9	0.0	0.0	0.0	7.279662806018825E-4	1.455932561203765E-4
10	0.0	0.0	0.0	7.279662806018825E-4	1.455932561203765E-4
11	0.0	0.0	0.0	7.279662806018825E-4	1.455932561203765E-4
12	0.0	0.0	0.0	7.279662806018825E-4	4.3677976836112954E-4
13	0.0	0.0	0.0	7.279662806018825E-4	4.3677976836112954E-4
14	0.0	0.0	0.0	7.279662806018825E-4	4.3677976836112954E-4
15	0.0	0.0	0.0	7.279662806018825E-4	7.279662806018825E-4
16	0.0	0.0	0.0	7.279662806018825E-4	7.279662806018825E-4
17	0.0	0.0	0.0	8.735595367222591E-4	7.279662806018825E-4
18	0.0	0.0	0.0	0.001164746048963012	7.279662806018825E-4
19	0.0	0.0	0.0	0.001164746048963012	7.279662806018825E-4
20	0.0	0.0	0.0	0.0017471190734445181	8.735595367222591E-4
21	1.455932561203765E-4	0.0	0.0	0.00334864489076866	0.0010191527928426355
22	1.455932561203765E-4	0.0	0.0	0.004804577451972425	0.0010191527928426355
23	1.455932561203765E-4	0.0	0.0	0.00960915490394485	0.0010191527928426355
24	1.455932561203765E-4	0.0	0.0	0.014704918868158027	0.0010191527928426355
25	1.455932561203765E-4	0.0	0.0	0.01863593678340819	0.0010191527928426355
26	1.455932561203765E-4	0.0	0.0	0.023003734467019487	0.0010191527928426355
27	1.455932561203765E-4	0.0	0.0	0.028827464711834547	0.0010191527928426355
28	1.455932561203765E-4	0.0	0.0	0.03974695892086279	0.0010191527928426355
29	1.455932561203765E-4	0.0	0.0	0.05110323289825215	0.0010191527928426355
30	1.455932561203765E-4	0.0	0.0	0.0749805269019939	0.0010191527928426355
31	1.455932561203765E-4	0.0	0.0	0.1139995195422548	0.0010191527928426355
32	1.455932561203765E-4	0.0	0.0	0.1640835996476643	0.0010191527928426355
33	1.455932561203765E-4	0.0	0.0	0.21475005277755535	0.0010191527928426355
34	1.455932561203765E-4	0.0	0.0	0.27604481360423383	0.0010191527928426355
35	1.455932561203765E-4	0.0	0.0	0.3469487293348572	0.0010191527928426355
36	1.455932561203765E-4	0.0	0.0	0.4533773995588524	0.0010191527928426355
37	1.455932561203765E-4	0.0	0.0	0.5874687884457191	0.0010191527928426355
38	1.455932561203765E-4	0.0	0.0	0.7586864576432819	0.0010191527928426355
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTATG	35	3.2165195E-4	31.428415	32
ATACGAA	180	0.0	25.66654	40
CGCAATA	210	0.0	25.142733	36
GTAATAC	55	1.5926953E-4	23.99988	3
TATGCGT	75	2.0621974E-6	23.46655	39
GTAAACG	115	3.5106495E-10	22.956408	27
AATACGA	205	0.0	22.536474	39
TAACGCC	200	0.0	21.99989	4
ATAACGC	205	0.0	21.463308	3
AACGCTT	145	1.2732926E-11	21.241274	30
CGAATGC	220	0.0	20.999895	43
CAATACG	220	0.0	20.999895	38
GCGCAAT	255	0.0	20.705778	35
GCATCGC	215	0.0	20.465015	14
ATTTAAC	65	4.929348E-4	20.30759	3
TACGAAT	230	0.0	20.086857	41
AACGTAT	55	0.004481804	19.9999	31
CAACGTA	55	0.004481804	19.9999	30
CGCATCG	220	0.0	19.9999	13
CATCGTT	220	0.0	19.9999	28
>>END_MODULE