FastQCFastQC Report
Thu 2 Feb 2017
SRR4062543_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062543_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090664
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28960.26552632158024836No Hit
GCGCAAGACGGACCAGAGCGAAAGC20350.18658358577893833No Hit
GATTAAGAGGGACGGCCGGGGGCAT19590.1796153535827716No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19250.17649798654764437No Hit
TATCAACGCAGAGTACTTTTTTTTT19200.17603955021894918No Hit
GAATAACGCCGCCGCATCGCCAGTC19120.1753060520930369No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19010.1742974921699075No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG18940.17365568130973424No Hit
GAATAGGACCGCGGTTCTATTTTGT18700.17145518693199738No Hit
GAACTACGACGGTATCTGATCGTCT18500.16962144161721668No Hit
GTATCTGATCGTCTTCGAACCTCCG17220.15788547160262006No Hit
ATCAGATACCGTCGTAGTTCCGACC15650.1434905708815914No Hit
GTCCTATTCCATTATTCCTAGCTGC15620.1432155090843743No Hit
GGGTAGGCACACGCTGAGCCAGTCA15280.14009814204924706No Hit
CTCTTAATCATGGCCTCAGTTCCGA14980.13734752407707598No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14840.1360639023567295No Hit
TCGTAGTTCCGACCATAAACGATGC14600.13386340797899263No Hit
GTGCATGGCCGTTCTTAGTTGGTGG14340.13147953906977766No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG13470.12350274695048154No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC13440.12322768515326443No Hit
GAATAATGGAATAGGACCGCGGTTC13260.12157731436996178No Hit
GCTTTGAACACTCTAATTTTTTCAA13130.12038537991535432No Hit
CTATTGGAGCTGGAATTACCGCGGC12590.11543426756544636No Hit
GTACATGGGGAATAATTGCAATCCC12280.11259196232753625No Hit
CATCTAAGGGCATCACAGACCTGTT12230.11213352599884108No Hit
GTACATGGAAGCAGTGGTATCAACG12090.11084990427849457No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12040.11039146794979939No Hit
CTTTAATATACGCTATTGGAGCTGG11980.10984134435536516No Hit
GAATAATTGCAATCCCCGATCCCCA11700.10727410091467217No Hit
GTTCTATTTTGTTGGTTTTCGGAAC11660.10690735185171603No Hit
CCATTATTCCTAGCTGCGGTATCCA11660.10690735185171603No Hit
GTTATTGCTCAATCTCGGGTGGCTG11310.10369829755084976No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT11190.10259805036198133No Hit
GGATTGACAGATTGATAGCTCTTTC11100.10177286497032999No Hit
ATATTAAAGTTGCTGCAGTTAAAAA10940.10030586871850543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCA400.00527852414.249699
ATTATCC400.00528495714.2470743
GCATTAC1101.8553692E-1013.8185157
GCGTTAT1700.013.4047051
TGGCCGC500.001498772213.3003226
AGAATTT3800.013.2539717
CTATCCT1001.4412763E-712.3497324
ACTCTAA2400.012.27000310
CGCCAGT3500.012.21794618
GCACGCC1405.2750693E-1112.2151415
TCGCGTA1405.2750693E-1112.214029
CGGTCCA4050.012.19670610
CGTTATT1800.012.1352832
TCGCCAG3450.012.11957217
GCAAGAC5450.012.0250543
CGCAAGA4900.012.0168382
CAAGACG4800.011.8747424
GCTCGTA1603.6379788E-1211.8747429
AGGCCCG3600.011.87419710
TTAGGAC2500.011.7775813
Produced by FastQC (version 0.11.5)