##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062543_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1090664
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.139825830870002	32.0	32.0	32.0	32.0	32.0
2	30.80330514255536	32.0	32.0	32.0	32.0	32.0
3	30.790613791231763	32.0	32.0	32.0	32.0	32.0
4	30.84366404318837	32.0	32.0	32.0	32.0	32.0
5	30.66196830554598	32.0	32.0	32.0	32.0	32.0
6	34.30631615236223	36.0	36.0	36.0	32.0	36.0
7	34.15508167501632	36.0	36.0	36.0	32.0	36.0
8	34.16382405580454	36.0	36.0	36.0	32.0	36.0
9	34.29095578473297	36.0	36.0	36.0	32.0	36.0
10	33.98332942134333	36.0	36.0	36.0	32.0	36.0
11	34.31350260025086	36.0	36.0	36.0	32.0	36.0
12	34.05728345301578	36.0	36.0	36.0	32.0	36.0
13	34.21844582749591	36.0	36.0	36.0	32.0	36.0
14	34.11781905334732	36.0	36.0	36.0	32.0	36.0
15	34.05542953650254	36.0	36.0	36.0	32.0	36.0
16	34.051417301753794	36.0	36.0	36.0	32.0	36.0
17	33.97740550710393	36.0	36.0	36.0	32.0	36.0
18	33.999249998166256	36.0	36.0	36.0	32.0	36.0
19	33.97734224289057	36.0	36.0	36.0	32.0	36.0
20	33.976514306880944	36.0	36.0	36.0	32.0	36.0
21	33.93803407832293	36.0	36.0	36.0	32.0	36.0
22	33.89020816676813	36.0	36.0	36.0	32.0	36.0
23	33.87242542157805	36.0	36.0	36.0	32.0	36.0
24	33.84983642991792	36.0	36.0	36.0	32.0	36.0
25	33.42118562637072	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	48.0
5	103.0
6	216.0
7	51.0
8	177.0
9	161.0
10	133.0
11	38.0
12	59.0
13	68.0
14	182.0
15	229.0
16	365.0
17	471.0
18	689.0
19	1026.0
20	1602.0
21	2436.0
22	3592.0
23	5250.0
24	7138.0
25	9703.0
26	13584.0
27	17159.0
28	23452.0
29	30761.0
30	39312.0
31	53443.0
32	76842.0
33	110319.0
34	247474.0
35	444572.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.52154091049134	17.950789307668607	10.906530127198838	25.62113965464122
2	17.437588309779475	20.614127180752984	36.208545406699486	25.739739102768056
3	19.015696878504382	24.574660347203693	28.366063053422934	28.043579720868987
4	12.478220839767738	15.268704401316102	36.244832607667	36.008242151249156
5	14.831022669424481	36.60926314051557	32.88382779859388	15.67588639146606
6	33.36696895920977	36.01843476920762	17.04518976914341	13.569406502439193
7	29.945262138024553	30.7096287624252	21.063250397734883	18.28185870181536
8	28.474991878394327	31.405285559326263	20.042746259014066	20.076976303265344
9	27.86425159966373	13.734806406731254	19.015165142565557	39.38577685103946
10	16.51197608912968	25.79561383136631	30.63708773530608	27.055322344197936
11	38.02126745513896	21.22100601819043	21.89745576317925	18.860270763491357
12	24.999563904514368	24.16483124022802	28.08124412992524	22.754360725332372
13	29.041591851272504	19.336061570774447	25.700418966207312	25.921927611745744
14	23.75521662416254	20.309261125891165	24.56224421787817	31.373278032068125
15	25.51929246430424	27.186058572720224	22.27975564867383	25.014893314301712
16	25.924736029020973	25.726281623507575	23.85289773723425	24.4960846102372
17	23.971211926487598	25.717682266326396	25.03304753336883	25.278058273817173
18	25.25143470409429	24.10816994094096	26.747327745997474	23.893067608967282
19	25.644794500299756	24.969083465920203	25.29931887226032	24.086803161519725
20	25.804571216647176	23.7466240964696	24.9934363806275	25.45536830625573
21	27.64016782497728	23.751090219696483	24.069021235184486	24.53972072014175
22	26.315045196930797	24.336187296375066	24.70468644973609	24.64408105695805
23	24.224642677775094	24.037155818841054	25.676885404395932	26.06131609898792
24	24.628714234824475	25.32960333753218	25.11384627814482	24.927836149498525
25	24.507378195282325	24.67667318813503	25.23789894264076	25.578049673941884
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	155.0
1	155.0
2	463.5
3	772.0
4	772.0
5	772.0
6	1987.5
7	3203.0
8	3203.0
9	3203.0
10	3336.5
11	3470.0
12	3470.0
13	3470.0
14	3561.0
15	3652.0
16	3652.0
17	3652.0
18	6007.0
19	8362.0
20	8362.0
21	8362.0
22	13329.0
23	18296.0
24	18296.0
25	18296.0
26	27349.0
27	36402.0
28	36402.0
29	36402.0
30	43518.0
31	50634.0
32	50634.0
33	50634.0
34	61832.0
35	73030.0
36	73030.0
37	73030.0
38	79754.0
39	86478.0
40	86478.0
41	86478.0
42	97627.0
43	108776.0
44	108776.0
45	108776.0
46	123531.5
47	138287.0
48	138287.0
49	138287.0
50	137224.0
51	136161.0
52	136161.0
53	136161.0
54	122472.5
55	108784.0
56	108784.0
57	108784.0
58	101632.5
59	94481.0
60	94481.0
61	94481.0
62	87336.0
63	80191.0
64	80191.0
65	80191.0
66	69562.0
67	58933.0
68	58933.0
69	58933.0
70	45720.0
71	32507.0
72	32507.0
73	32507.0
74	27011.5
75	21516.0
76	21516.0
77	21516.0
78	18193.0
79	14870.0
80	14870.0
81	14870.0
82	10699.5
83	6529.0
84	6529.0
85	6529.0
86	4949.5
87	3370.0
88	3370.0
89	3370.0
90	2253.0
91	1136.0
92	1136.0
93	1136.0
94	674.0
95	212.0
96	212.0
97	212.0
98	334.5
99	457.0
100	457.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00806847938503517
2	0.00394255242677855
3	0.005959672273037342
4	0.0157702097071142
5	0.03933383700204646
6	0.050427996156469826
7	0.06803195117836475
8	0.08967014589277725
9	0.0971885016833782
10	0.1178181364746613
11	0.11919344546074684
12	0.13322159711881937
13	0.12066044171257143
14	0.12451130687361094
15	0.114517394908056
16	0.11415064584509987
17	0.1213939398384837
18	0.129737481020736
19	0.13212134992995092
20	0.12157731436996178
21	0.13147953906977766
22	0.15146736300088753
23	0.13945633118907383
24	0.1353304042308172
25	0.13212134992995092
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1090664.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.61068127742343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56537356871932	37.55206949408131
2	12.418846337303348	11.577017769226874
3	3.2145095280678535	4.494914372280347
4	1.1969685992610137	2.2316608751695632
5	0.567943513373759	1.323611704272218
6	0.32352093155057143	0.9047718616247283
7	0.2255152302252596	0.7357992963464041
8	0.16256801233458254	0.6061924647065176
9	0.1095896896245904	0.4597245084945186
>10	0.8900795623746188	8.435202820471542
>50	0.14566967861330776	4.831822997658076
>100	0.1509931151848906	15.121531860042579
>500	0.02072454516335856	6.464004758129437
>1k	0.007697688203533179	5.2616752174959
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2896	0.26552632158024836	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2035	0.18658358577893833	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1959	0.1796153535827716	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1925	0.17649798654764437	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1920	0.17603955021894918	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1912	0.1753060520930369	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1901	0.1742974921699075	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1894	0.17365568130973424	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1870	0.17145518693199738	No Hit
GAACTACGACGGTATCTGATCGTCT	1850	0.16962144161721668	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1722	0.15788547160262006	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1565	0.1434905708815914	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1562	0.1432155090843743	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1528	0.14009814204924706	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1498	0.13734752407707598	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1484	0.1360639023567295	No Hit
TCGTAGTTCCGACCATAAACGATGC	1460	0.13386340797899263	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1434	0.13147953906977766	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1347	0.12350274695048154	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1344	0.12322768515326443	No Hit
GAATAATGGAATAGGACCGCGGTTC	1326	0.12157731436996178	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1313	0.12038537991535432	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1259	0.11543426756544636	No Hit
GTACATGGGGAATAATTGCAATCCC	1228	0.11259196232753625	No Hit
CATCTAAGGGCATCACAGACCTGTT	1223	0.11213352599884108	No Hit
GTACATGGAAGCAGTGGTATCAACG	1209	0.11084990427849457	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1204	0.11039146794979939	No Hit
CTTTAATATACGCTATTGGAGCTGG	1198	0.10984134435536516	No Hit
GAATAATTGCAATCCCCGATCCCCA	1170	0.10727410091467217	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	1166	0.10690735185171603	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1166	0.10690735185171603	No Hit
GTTATTGCTCAATCTCGGGTGGCTG	1131	0.10369829755084976	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1119	0.10259805036198133	No Hit
GGATTGACAGATTGATAGCTCTTTC	1110	0.10177286497032999	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1094	0.10030586871850543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.168726573903603E-5	9.168726573903603E-5
5	0.0	9.168726573903603E-5	0.0	9.168726573903603E-5	9.168726573903603E-5
6	0.0	9.168726573903603E-5	0.0	9.168726573903603E-5	1.8337453147807207E-4
7	0.0	9.168726573903603E-5	0.0	9.168726573903603E-5	1.8337453147807207E-4
8	9.168726573903603E-5	9.168726573903603E-5	0.0	9.168726573903603E-5	1.8337453147807207E-4
9	9.168726573903603E-5	9.168726573903603E-5	0.0	1.8337453147807207E-4	1.8337453147807207E-4
10	9.168726573903603E-5	9.168726573903603E-5	0.0	1.8337453147807207E-4	1.8337453147807207E-4
11	9.168726573903603E-5	9.168726573903603E-5	0.0	1.8337453147807207E-4	1.8337453147807207E-4
12	9.168726573903603E-5	9.168726573903603E-5	0.0	1.8337453147807207E-4	1.8337453147807207E-4
13	9.168726573903603E-5	1.8337453147807207E-4	0.0	2.750617972171081E-4	1.8337453147807207E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGCA	40	0.005278524	14.24969	9
ATTATCC	40	0.005284957	14.247074	3
GCATTAC	110	1.8553692E-10	13.818515	7
GCGTTAT	170	0.0	13.404705	1
TGGCCGC	50	0.0014987722	13.300322	6
AGAATTT	380	0.0	13.25397	17
CTATCCT	100	1.4412763E-7	12.349732	4
ACTCTAA	240	0.0	12.270003	10
CGCCAGT	350	0.0	12.217946	18
GCACGCC	140	5.2750693E-11	12.21514	15
TCGCGTA	140	5.2750693E-11	12.21402	9
CGGTCCA	405	0.0	12.196706	10
CGTTATT	180	0.0	12.135283	2
TCGCCAG	345	0.0	12.119572	17
GCAAGAC	545	0.0	12.025054	3
CGCAAGA	490	0.0	12.016838	2
CAAGACG	480	0.0	11.874742	4
GCTCGTA	160	3.6379788E-12	11.874742	9
AGGCCCG	360	0.0	11.874197	10
TTAGGAC	250	0.0	11.777581	3
>>END_MODULE