FastQCFastQC Report
Thu 2 Feb 2017
SRR4062543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090664
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20130.18456646593267953No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20110.1843830914012015No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18870.173013870449561No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18660.17108843786904127No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17530.16072777684053016No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA16800.15403460644158054No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG16720.15330110831566826No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16550.15174242479810462No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC16230.1488084322944555No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15740.14431575627324272No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14470.13267147352438513No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT13570.1244196196078719No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT13190.12093550350978854No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG13090.12001863085239818No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC12990.11910175819500782No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12800.11735970014596614No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA11950.10956628255814807No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC11920.10929122076093097No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT11800.10819097357206253No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11740.10764084997762831No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG11600.1063572282572818No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC11090.10168117770459097No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11040.10122274137589578No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10950.10039755598424446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAT752.0631742E-623.4676951
TGCGTAC703.2150332E-521.99995416
GATCGAA500.00258030921.99995211
TATACTG1004.6231435E-821.9999525
ATTCGTG2300.021.04343644
TAGGACG3150.020.9523374
TAGTACG1201.4302714E-820.167552
TGCGTAG1201.4311809E-820.16662413
TCTATAC1101.26538E-719.9999583
AAACGCT4200.019.38091329
CGCTTCG4200.019.38091332
AGGACGT3200.019.2499585
CGTACTT808.9876914E-519.24995818
TAAACGC4300.018.93019328
GTACGCC1404.0799932E-918.8571054
TGTAGGA3650.018.685752
CGTCGTA4300.018.41856610
CGCGGGA4200.018.33329644
TAAGCGC600.007411738418.33329418
GTAAACG4350.018.2068627
Produced by FastQC (version 0.11.5)