##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062543_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1090664
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272037034320377	32.0	32.0	32.0	32.0	32.0
2	31.444939963178395	32.0	32.0	32.0	32.0	32.0
3	31.521394306587545	32.0	32.0	32.0	32.0	32.0
4	31.613705962606264	32.0	32.0	32.0	32.0	32.0
5	31.56229966332436	32.0	32.0	32.0	32.0	32.0
6	35.14986742021374	36.0	36.0	36.0	36.0	36.0
7	35.15672746143633	36.0	36.0	36.0	36.0	36.0
8	35.101531727461435	36.0	36.0	36.0	36.0	36.0
9	35.19733850205013	36.0	36.0	36.0	36.0	36.0
10	35.086007239626504	36.0	36.0	36.0	36.0	36.0
11	35.2268746378353	36.0	36.0	36.0	36.0	36.0
12	35.118161963721185	36.0	36.0	36.0	36.0	36.0
13	35.1780933449715	36.0	36.0	36.0	36.0	36.0
14	35.13189396551092	36.0	36.0	36.0	36.0	36.0
15	35.10499017112511	36.0	36.0	36.0	36.0	36.0
16	35.116098083369394	36.0	36.0	36.0	36.0	36.0
17	35.080620612764335	36.0	36.0	36.0	36.0	36.0
18	35.09448556108939	36.0	36.0	36.0	36.0	36.0
19	35.06602950129462	36.0	36.0	36.0	36.0	36.0
20	35.058071963501135	36.0	36.0	36.0	36.0	36.0
21	35.04521740884452	36.0	36.0	36.0	36.0	36.0
22	35.0330816823513	36.0	36.0	36.0	36.0	36.0
23	35.00258374714853	36.0	36.0	36.0	36.0	36.0
24	34.972842231888094	36.0	36.0	36.0	36.0	36.0
25	34.944079019753104	36.0	36.0	36.0	32.0	36.0
26	34.91461256628989	36.0	36.0	36.0	32.0	36.0
27	34.89995910747948	36.0	36.0	36.0	32.0	36.0
28	34.87671088437869	36.0	36.0	36.0	32.0	36.0
29	34.856346225785394	36.0	36.0	36.0	32.0	36.0
30	34.83616952608686	36.0	36.0	36.0	32.0	36.0
31	34.83589079679901	36.0	36.0	36.0	32.0	36.0
32	34.7955062237316	36.0	36.0	36.0	32.0	36.0
33	34.77057370555918	36.0	36.0	36.0	32.0	36.0
34	34.75400948413077	36.0	36.0	36.0	32.0	36.0
35	34.718076327814984	36.0	36.0	36.0	32.0	36.0
36	34.691680480881374	36.0	36.0	36.0	32.0	36.0
37	34.67655758327037	36.0	36.0	36.0	32.0	36.0
38	34.62630104230084	36.0	36.0	36.0	32.0	36.0
39	34.62489089215377	36.0	36.0	36.0	32.0	36.0
40	34.612888112195876	36.0	36.0	36.0	32.0	36.0
41	34.57210653326781	36.0	36.0	36.0	32.0	36.0
42	34.533188956452214	36.0	36.0	36.0	32.0	36.0
43	34.54603617612757	36.0	36.0	36.0	32.0	36.0
44	34.47758154665415	36.0	36.0	36.0	32.0	36.0
45	34.43365326076592	36.0	36.0	36.0	32.0	36.0
46	34.42750012836217	36.0	36.0	36.0	32.0	36.0
47	34.39851503304409	36.0	36.0	36.0	32.0	36.0
48	34.34774412651376	36.0	36.0	36.0	32.0	36.0
49	34.32691002911987	36.0	36.0	36.0	32.0	36.0
50	33.80142922109834	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	9.0
21	18.0
22	102.0
23	301.0
24	772.0
25	1746.0
26	3700.0
27	6648.0
28	11385.0
29	17233.0
30	24940.0
31	35390.0
32	52134.0
33	84780.0
34	185721.0
35	665782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.210085181812595	18.573743387153126	11.325723917024533	25.890447514009747
2	16.8760865259776	21.217239178751406	35.86911075413155	26.03756354113945
3	18.38391432710469	24.894467570095173	28.724808831349826	27.996809271450314
4	12.175885515612507	15.775160819464107	36.41304746466373	35.63590620025966
5	14.345848033858275	36.94034092992892	32.932690544475655	15.781120491737145
6	33.36621871037366	36.28656855455014	16.800332276783628	13.54688045829257
7	29.863826072924383	30.817373636610355	21.061848561976923	18.256951728488332
8	28.472746877131726	31.70105550380319	19.830855332164628	19.99534228690046
9	28.027880263765926	13.543859520438925	18.98760754916271	39.44065266663244
10	16.613549177381852	25.89120022298343	30.56257472512158	26.93267587451314
11	38.1388768676696	21.15436101310761	22.003751842914042	18.703010276308742
12	24.9544314289277	24.178757160775454	28.166419722297608	22.70039168799924
13	29.28858016767767	19.256617986841043	25.54471404575561	25.910087799725673
14	23.875363998444985	20.252158318235498	24.62050640710613	31.251971276213393
15	25.6125626224025	27.135579793593628	22.25983437612317	24.992023207880703
16	26.151408683150812	25.708009066036837	23.75085269157137	24.389729559240976
17	24.039850953180814	25.70094914657493	25.00403423969252	25.255165660551736
18	25.368766182802403	24.002809297822246	26.728488333712306	23.899936185663044
19	25.751010393668444	24.719987090432983	25.463570815576563	24.06543170032201
20	25.943737026251902	23.680345184218055	24.96185809745256	25.414059692077483
21	27.899609778997014	23.690797532512303	24.087620018630854	24.32197266985983
22	26.34578568651757	24.212589761833158	24.804797811241592	24.636826740407678
23	24.279338091291176	23.946421629392738	25.677202144748517	26.09703813456757
24	24.733556805762362	25.186766960310415	25.124236245076393	24.955439988850827
25	24.755378375008252	24.374326098596818	25.394897053538028	25.475398472856902
26	24.225334291770885	25.517666302362596	25.963816537448746	24.29318286841777
27	25.783467685740064	24.416777302633992	24.85540918192954	24.944345829696406
28	24.11008339873692	24.612437927721096	26.132062670079875	25.145416003462113
29	24.285939574424386	24.818184152039493	25.73744067833907	25.158435595197055
30	23.97374443458297	25.11149171513867	26.401439856821167	24.5133239934572
31	24.902719811050883	24.918673395289474	24.543030667556646	25.635576126103
32	24.63847711119098	24.784993361841963	24.712377047376645	25.864152479590413
33	24.12750397922733	24.144282748857577	25.808956745615514	25.919256526299577
34	25.224633801060637	24.503696830554595	25.829861442204017	24.441807926180747
35	26.147557817989775	24.220474866686715	25.543430424035268	24.088536891288243
36	24.142265629011316	25.154493042770277	25.212439394717347	25.49080193350106
37	25.751652204528618	24.879614620084645	24.757212120323032	24.611521055063704
38	24.270994550108924	24.43786537375397	25.825002017119846	25.466138059017258
39	25.378576720236477	24.23927075616322	24.895843266120455	25.486309257479846
40	25.527018403467977	24.58346475174756	25.762838050948776	24.126678793835683
41	24.015462140494233	25.23866195271871	26.28004591698268	24.465829989804377
42	25.88148137281509	25.362256386934934	25.4582529541637	23.298009286086273
43	24.598868212391718	23.89269289166966	25.76943953408199	25.738999361856628
44	24.062314333286878	24.840280783082598	25.390496064782553	25.706908818847968
45	24.440157555397445	25.285605832777097	25.329615720331834	24.94462089149362
46	23.953342098652012	24.600655381777305	25.706680896556406	25.739321623014277
47	24.28364739278091	24.15656884246661	26.33359128017428	25.226192484578203
48	25.17585617568747	25.319896870163493	24.963416780970125	24.540830173178907
49	23.66741727974885	25.591107802219565	25.380502152816998	25.360972765214584
50	23.67550746655933	25.857391329860828	25.20301871430497	25.264082489274877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	12.0
2	14.0
3	60.5
4	107.0
5	113.5
6	120.0
7	141.0
8	162.0
9	229.0
10	296.0
11	427.5
12	559.0
13	995.5
14	1432.0
15	2203.5
16	2975.0
17	3637.5
18	4300.0
19	4876.0
20	5452.0
21	6061.5
22	6671.0
23	6961.5
24	7252.0
25	8241.0
26	9230.0
27	10800.0
28	12370.0
29	14410.5
30	16451.0
31	18058.5
32	19666.0
33	22354.0
34	25042.0
35	28390.5
36	31739.0
37	38408.0
38	45077.0
39	47360.5
40	49644.0
41	53602.5
42	57561.0
43	56895.5
44	56230.0
45	61925.0
46	67620.0
47	71788.0
48	75956.0
49	78628.5
50	81301.0
51	74712.5
52	68124.0
53	65758.0
54	63392.0
55	66310.5
56	69229.0
57	68711.5
58	68194.0
59	62311.0
60	56428.0
61	49626.5
62	42825.0
63	38393.0
64	33961.0
65	30249.5
66	26538.0
67	24696.5
68	22855.0
69	21922.5
70	20990.0
71	16698.5
72	12407.0
73	11399.5
74	10392.0
75	7729.5
76	5067.0
77	4703.5
78	4340.0
79	3786.5
80	3233.0
81	2632.5
82	2032.0
83	1810.0
84	1588.0
85	1318.0
86	1048.0
87	808.5
88	569.0
89	364.0
90	159.0
91	94.5
92	30.0
93	21.5
94	13.0
95	9.5
96	6.0
97	5.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015861896972853236
2	0.0029339925036491535
3	3.667490629561442E-4
4	0.0
5	0.0
6	6.418108601732522E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.833745314780721E-4
47	0.0
48	0.0
49	0.0
50	9.168726573903605E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1090664.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.99298350746197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.37287706883627	41.494457783258994
2	12.139037963353761	12.380115253235065
3	3.016730087665084	4.614962028203099
4	1.0876881359269368	2.218578527063374
5	0.5482125265445237	1.3977496162334477
6	0.31980981727077046	0.9784834042567695
7	0.20039878508680045	0.7153252359992642
8	0.15166409796599398	0.6187043877003222
9	0.11628638114669747	0.5336810564360384
>10	0.7843768776244536	8.039884230824306
>50	0.11699881060049949	4.243797160337783
>100	0.1238597547250986	13.411265526970315
>500	0.016635178518792527	5.544322540047072
>1k	0.0054245147343888675	3.808673249434142
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2013	0.18456646593267953	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2011	0.1843830914012015	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1887	0.173013870449561	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1866	0.17108843786904127	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1753	0.16072777684053016	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1680	0.15403460644158054	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1672	0.15330110831566826	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1655	0.15174242479810462	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1623	0.1488084322944555	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1574	0.14431575627324272	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1447	0.13267147352438513	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	1357	0.1244196196078719	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1319	0.12093550350978854	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	1309	0.12001863085239818	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	1299	0.11910175819500782	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1280	0.11735970014596614	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	1195	0.10956628255814807	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC	1192	0.10929122076093097	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	1180	0.10819097357206253	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1174	0.10764084997762831	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	1160	0.1063572282572818	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1109	0.10168117770459097	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1104	0.10122274137589578	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1095	0.10039755598424446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.8337453147807207E-4
11	0.0	9.168726573903603E-5	0.0	0.0	1.8337453147807207E-4
12	0.0	9.168726573903603E-5	0.0	0.0	1.8337453147807207E-4
13	0.0	9.168726573903603E-5	0.0	9.168726573903603E-5	1.8337453147807207E-4
14	0.0	9.168726573903603E-5	0.0	9.168726573903603E-5	1.8337453147807207E-4
15	0.0	9.168726573903603E-5	0.0	9.168726573903603E-5	3.6674906295614413E-4
16	0.0	9.168726573903603E-5	0.0	1.8337453147807207E-4	4.584363286951802E-4
17	0.0	9.168726573903603E-5	0.0	1.8337453147807207E-4	4.584363286951802E-4
18	0.0	9.168726573903603E-5	0.0	1.8337453147807207E-4	5.501235944342162E-4
19	0.0	9.168726573903603E-5	0.0	1.8337453147807207E-4	5.501235944342162E-4
20	0.0	9.168726573903603E-5	9.168726573903603E-5	5.501235944342162E-4	5.501235944342162E-4
21	0.0	9.168726573903603E-5	9.168726573903603E-5	8.251853916513243E-4	6.418108601732522E-4
22	0.0	9.168726573903603E-5	9.168726573903603E-5	0.0013753089860855405	6.418108601732522E-4
23	0.0	9.168726573903603E-5	9.168726573903603E-5	0.002108807111997829	6.418108601732522E-4
24	0.0	9.168726573903603E-5	9.168726573903603E-5	0.0036674906295614414	6.418108601732522E-4
25	0.0	9.168726573903603E-5	9.168726573903603E-5	0.004584363286951802	7.334981259122883E-4
26	0.0	9.168726573903603E-5	9.168726573903603E-5	0.005409548678603126	7.334981259122883E-4
27	0.0	9.168726573903603E-5	9.168726573903603E-5	0.007701730322079027	7.334981259122883E-4
28	0.0	9.168726573903603E-5	9.168726573903603E-5	0.017970704084851064	7.334981259122883E-4
29	0.0	9.168726573903603E-5	9.168726573903603E-5	0.037683466218743814	7.334981259122883E-4
30	0.0	9.168726573903603E-5	9.168726573903603E-5	0.07903442306704907	7.334981259122883E-4
31	0.0	9.168726573903603E-5	9.168726573903603E-5	0.1664123873163504	7.334981259122883E-4
32	0.0	9.168726573903603E-5	9.168726573903603E-5	0.2643343871256409	7.334981259122883E-4
33	0.0	9.168726573903603E-5	9.168726573903603E-5	0.35501309294154754	7.334981259122883E-4
34	0.0	9.168726573903603E-5	9.168726573903603E-5	0.46421262643673944	7.334981259122883E-4
35	0.0	9.168726573903603E-5	9.168726573903603E-5	0.5887239333103504	7.334981259122883E-4
36	0.0	9.168726573903603E-5	9.168726573903603E-5	0.7653136071237338	8.251853916513243E-4
37	0.0	9.168726573903603E-5	9.168726573903603E-5	1.0087432976608746	8.251853916513243E-4
38	0.0	9.168726573903603E-5	9.168726573903603E-5	1.3414763850278362	9.168726573903604E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTTAT	75	2.0631742E-6	23.467695	1
TGCGTAC	70	3.2150332E-5	21.999954	16
GATCGAA	50	0.002580309	21.999952	11
TATACTG	100	4.6231435E-8	21.999952	5
ATTCGTG	230	0.0	21.043436	44
TAGGACG	315	0.0	20.952337	4
TAGTACG	120	1.4302714E-8	20.16755	2
TGCGTAG	120	1.4311809E-8	20.166624	13
TCTATAC	110	1.26538E-7	19.999958	3
AAACGCT	420	0.0	19.380913	29
CGCTTCG	420	0.0	19.380913	32
AGGACGT	320	0.0	19.249958	5
CGTACTT	80	8.9876914E-5	19.249958	18
TAAACGC	430	0.0	18.930193	28
GTACGCC	140	4.0799932E-9	18.857105	4
TGTAGGA	365	0.0	18.68575	2
CGTCGTA	430	0.0	18.418566	10
CGCGGGA	420	0.0	18.333296	44
TAAGCGC	60	0.0074117384	18.333294	18
GTAAACG	435	0.0	18.20686	27
>>END_MODULE