FastQCFastQC Report
Thu 2 Feb 2017
SRR4062542_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062542_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085605
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30900.2846339138084294No Hit
GTACATGGAAGCAGTGGTATCAACG27000.24870924507532666No Hit
TCCATGTACTCTGCGTTGATACCAC22990.21177131645488No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22650.20863942225763515No Hit
TATCAACGCAGAGTACTTTTTTTTT19580.18036025994721838No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16220.14940977611562217No Hit
GTCCTAAAGTGTGTATTTCTCATTT14970.1378954592139867No Hit
GTCCTACAGTGGACATTTCTAAATT14000.1289603492983175No Hit
CTGTAGGACGTGGAATATGGCAAGA13430.12370982079117174No Hit
CTTTAGGACGTGAAATATGGCGAGG12620.11624854343891194No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12010.11062955679091382No Hit
GAGTACATGGAAGCAGTGGTATCAA12010.11062955679091382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAG402.7603045E-416.6248785
AACCGCG456.758006E-414.7783517
ACGATCA653.3652723E-614.6172999
GTCTAAC400.005307136614.2380751
ACAATAC1351.8189894E-1213.367193
CTAGTAG500.00150172713.2968373
CGAACGA655.440469E-513.156177516
TTCTCGT1403.6379788E-1212.89454711
CTAAAAC751.4812258E-512.6636533
ATCGCCA1155.302354E-912.39350116
TAACGAA701.0894495E-412.21532313
CGTCTTA1251.4042598E-912.16159415
CGCGCGC550.00306440612.09249414
ACGCGCG550.003065465312.09193613
CTAATCC550.003072886712.0880333
AATACGA802.8647017E-511.8749135
GTATTAG1701.8189894E-1211.7254731
CTATACT907.4667278E-611.609424
CAATACG907.4667278E-611.609424
CAAGACG1900.011.4983254