##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062542_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1085605
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.115606505128476	32.0	32.0	32.0	32.0	32.0
2	30.815593148520872	32.0	32.0	32.0	32.0	32.0
3	30.805617144357292	32.0	32.0	32.0	32.0	32.0
4	30.86891180493826	32.0	32.0	32.0	32.0	32.0
5	30.695690421470054	32.0	32.0	32.0	32.0	32.0
6	34.31275463911828	36.0	36.0	36.0	32.0	36.0
7	34.17527922218486	36.0	36.0	36.0	32.0	36.0
8	34.16690416864329	36.0	36.0	36.0	32.0	36.0
9	34.30935100704215	36.0	36.0	36.0	32.0	36.0
10	34.01516757936819	36.0	36.0	36.0	32.0	36.0
11	34.33056037877497	36.0	36.0	36.0	32.0	36.0
12	34.110521782784716	36.0	36.0	36.0	32.0	36.0
13	34.23084731555216	36.0	36.0	36.0	32.0	36.0
14	34.15120232497087	36.0	36.0	36.0	32.0	36.0
15	34.09821528088025	36.0	36.0	36.0	32.0	36.0
16	34.09550619239963	36.0	36.0	36.0	32.0	36.0
17	34.026807172037714	36.0	36.0	36.0	32.0	36.0
18	34.034607430879554	36.0	36.0	36.0	32.0	36.0
19	34.02247226201058	36.0	36.0	36.0	32.0	36.0
20	33.986846965516925	36.0	36.0	36.0	32.0	36.0
21	33.9606191939057	36.0	36.0	36.0	32.0	36.0
22	33.9110532836529	36.0	36.0	36.0	32.0	36.0
23	33.89668710074106	36.0	36.0	36.0	32.0	36.0
24	33.86317951741195	36.0	36.0	36.0	32.0	36.0
25	33.407459435061554	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	44.0
5	91.0
6	183.0
7	52.0
8	175.0
9	173.0
10	134.0
11	43.0
12	95.0
13	69.0
14	276.0
15	326.0
16	418.0
17	646.0
18	819.0
19	1087.0
20	1687.0
21	2326.0
22	3484.0
23	4902.0
24	7003.0
25	9280.0
26	13277.0
27	16687.0
28	22659.0
29	29752.0
30	38768.0
31	52737.0
32	75923.0
33	108224.0
34	241821.0
35	452438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.948363537206404	17.41583695067397	10.875605244345525	25.7601942677741
2	16.246916355154823	20.262793786835623	38.19601940370277	25.294270454306783
3	19.470997731110316	24.552278884586933	28.116084613095815	27.860638771206936
4	12.313938501705726	15.4604357042768	36.390529001041955	35.83509679297552
5	13.89838005197103	37.32035900554726	34.0446176812075	14.736643261274212
6	33.567841696717466	35.7182287092425	17.284839190973795	13.42909040306624
7	29.551948794147904	30.70710385334361	21.223255007945607	18.51769234456288
8	27.507564596398126	33.93373763896086	19.802370885569463	18.756326879071562
9	27.443000346681814	14.166654372985374	18.74414513428388	39.64620014604893
10	15.494346283561017	27.22415941257789	32.787774212855666	24.49372009100542
11	36.95808955356023	21.082769534967795	22.904477678011496	19.05466323346048
12	24.569141640679117	23.89867668720767	29.784084274373186	21.74809739774003
13	29.969270043641703	20.159662966529066	25.427471031574754	24.44359595825448
14	22.723910605456936	20.522064574202783	25.815293579470833	30.938731240869448
15	24.483016835613004	28.79105617281218	22.92035545128591	23.805571540288906
16	24.44406122565664	26.086563591059242	25.749781903425177	23.71959327985894
17	22.979190818616207	26.61109066745613	26.4401017817337	23.96961673219396
18	23.854192608170305	25.171329219587335	28.259774758571073	22.714703413671288
19	24.925795770036064	25.72068955975539	25.893079498602617	23.46043517160593
20	24.950058196266216	25.043670256819844	26.57448107560719	23.43179047130675
21	25.52900463565042	24.98934704290513	24.904123386146175	24.57752493529827
22	24.86540093730396	25.93647367061515	25.555555555555554	23.642569836525333
23	23.252450379017784	25.83537954355522	26.280441796926162	24.63172828050083
24	24.040755109608224	25.867558807306107	26.389911210237848	23.70177487284782
25	23.99581622761345	25.67633503014696	26.33821224897135	23.98963649326824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	237.0
1	237.0
2	513.0
3	789.0
4	789.0
5	789.0
6	2183.5
7	3578.0
8	3578.0
9	3578.0
10	3596.5
11	3615.0
12	3615.0
13	3615.0
14	3761.0
15	3907.0
16	3907.0
17	3907.0
18	6312.5
19	8718.0
20	8718.0
21	8718.0
22	13840.5
23	18963.0
24	18963.0
25	18963.0
26	28521.5
27	38080.0
28	38080.0
29	38080.0
30	48537.5
31	58995.0
32	58995.0
33	58995.0
34	68428.5
35	77862.0
36	77862.0
37	77862.0
38	85738.0
39	93614.0
40	93614.0
41	93614.0
42	105246.0
43	116878.0
44	116878.0
45	116878.0
46	132647.0
47	148416.0
48	148416.0
49	148416.0
50	144106.5
51	139797.0
52	139797.0
53	139797.0
54	126590.0
55	113383.0
56	113383.0
57	113383.0
58	102744.5
59	92106.0
60	92106.0
61	92106.0
62	80932.0
63	69758.0
64	69758.0
65	69758.0
66	57733.5
67	45709.0
68	45709.0
69	45709.0
70	34755.0
71	23801.0
72	23801.0
73	23801.0
74	18478.0
75	13155.0
76	13155.0
77	13155.0
78	10689.0
79	8223.0
80	8223.0
81	8223.0
82	5800.5
83	3378.0
84	3378.0
85	3378.0
86	2459.0
87	1540.0
88	1540.0
89	1540.0
90	1015.0
91	490.0
92	490.0
93	490.0
94	303.0
95	116.0
96	116.0
97	116.0
98	306.5
99	497.0
100	497.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008566651774816808
2	0.003592466873310274
3	0.004789955831080365
4	0.013264493070684088
5	0.03546409605703732
6	0.0458730385361158
7	0.06715149617033819
8	0.0872324648467905
9	0.09552277301596805
10	0.11735391786146895
11	0.11910409403051755
12	0.13190801442513622
13	0.12168330101648389
14	0.1227886754390409
15	0.11394568005858485
16	0.11265607656560167
17	0.12195964462212315
18	0.13218435803077547
19	0.13218435803077547
20	0.12370982079117174
21	0.12886823476310444
22	0.1515284104255231
23	0.13697431386185582
24	0.1355004812984465
25	0.13043418186172687
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1085605.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.480911396110976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91489511588775	45.361000939897345
2	13.538224403852395	15.563789548370139
3	3.821621607758478	6.590108790750847
4	1.4105934695145674	3.2432879294839845
5	0.6609037029147766	1.8994673594302964
6	0.36847494232900896	1.270816530702052
7	0.2226837089208123	0.8960043779284204
8	0.15434347046192157	0.7097442680151987
9	0.10882049414279306	0.562959106371261
>10	0.6246502853103968	6.915020388445662
>50	0.08668437738087206	3.528348423793097
>100	0.07990492359907307	9.037689527230485
>500	0.006109429828157434	2.3042663885739714
>1k	0.0020900680991064904	2.1174964210072753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3090	0.2846339138084294	No Hit
GTACATGGAAGCAGTGGTATCAACG	2700	0.24870924507532666	No Hit
TCCATGTACTCTGCGTTGATACCAC	2299	0.21177131645488	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2265	0.20863942225763515	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1958	0.18036025994721838	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1622	0.14940977611562217	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1497	0.1378954592139867	No Hit
GTCCTACAGTGGACATTTCTAAATT	1400	0.1289603492983175	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1343	0.12370982079117174	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1262	0.11624854343891194	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1201	0.11062955679091382	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1201	0.11062955679091382	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.842290704261679E-4
2	0.0	0.0	0.0	0.0	1.842290704261679E-4
3	0.0	0.0	0.0	0.0	1.842290704261679E-4
4	0.0	0.0	0.0	0.0	1.842290704261679E-4
5	0.0	0.0	0.0	0.0	1.842290704261679E-4
6	0.0	0.0	0.0	0.0	1.842290704261679E-4
7	0.0	0.0	0.0	0.0	1.842290704261679E-4
8	0.0	0.0	0.0	0.0	1.842290704261679E-4
9	0.0	0.0	0.0	0.0	1.842290704261679E-4
10	0.0	0.0	0.0	0.0	2.763436056392518E-4
11	0.0	9.211453521308395E-5	0.0	9.211453521308395E-5	2.763436056392518E-4
12	0.0	9.211453521308395E-5	0.0	9.211453521308395E-5	4.6057267606541976E-4
13	0.0	9.211453521308395E-5	0.0	1.842290704261679E-4	4.6057267606541976E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGATAG	40	2.7603045E-4	16.624878	5
AACCGCG	45	6.758006E-4	14.778351	7
ACGATCA	65	3.3652723E-6	14.617299	9
GTCTAAC	40	0.0053071366	14.238075	1
ACAATAC	135	1.8189894E-12	13.36719	3
CTAGTAG	50	0.001501727	13.296837	3
CGAACGA	65	5.440469E-5	13.1561775	16
TTCTCGT	140	3.6379788E-12	12.894547	11
CTAAAAC	75	1.4812258E-5	12.663653	3
ATCGCCA	115	5.302354E-9	12.393501	16
TAACGAA	70	1.0894495E-4	12.215323	13
CGTCTTA	125	1.4042598E-9	12.161594	15
CGCGCGC	55	0.003064406	12.092494	14
ACGCGCG	55	0.0030654653	12.091936	13
CTAATCC	55	0.0030728867	12.088033	3
AATACGA	80	2.8647017E-5	11.874913	5
GTATTAG	170	1.8189894E-12	11.725473	1
CTATACT	90	7.4667278E-6	11.60942	4
CAATACG	90	7.4667278E-6	11.60942	4
CAAGACG	190	0.0	11.498325	4
>>END_MODULE