FastQCFastQC Report
Thu 2 Feb 2017
SRR4062542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085605
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAT1700.022.0049061
CTAACGG500.002579689522.0008533
TTAACGG1158.769348E-921.04332435
GTCCTAC9050.020.1812951
TCCTACA9250.019.9791532
TCTAGCG2550.019.84299328
CTAGCGG2550.019.84299329
GCAATAC2750.019.1998637
GCCGGTC3350.019.0446368
TTAGGAC7900.018.3804593
CGCAATA2900.018.20676436
CCGGTCC3400.018.1175149
GTCCTAA5600.018.0754591
TAGCGGC2800.018.07129730
TAGAGTA1102.7960868E-617.9998684
TGTAGGA11150.017.9558532
GTAGGAC11050.017.9192453
ATACGAA2950.017.89817440
TAGACTG1601.1004886E-917.8748685
TAGGACG10600.017.8489274