##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062542_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1085605
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234845086380407	32.0	32.0	32.0	32.0	32.0
2	31.39431469088665	32.0	32.0	32.0	32.0	32.0
3	31.488657476706535	32.0	32.0	32.0	32.0	32.0
4	31.598874360379696	32.0	32.0	32.0	32.0	32.0
5	31.52512285776134	32.0	32.0	32.0	32.0	32.0
6	35.10696616172549	36.0	36.0	36.0	36.0	36.0
7	35.12545447008811	36.0	36.0	36.0	36.0	36.0
8	35.06522814467509	36.0	36.0	36.0	36.0	36.0
9	35.188696625384004	36.0	36.0	36.0	36.0	36.0
10	35.08067575223032	36.0	36.0	36.0	36.0	36.0
11	35.204358859806284	36.0	36.0	36.0	36.0	36.0
12	35.112663445728415	36.0	36.0	36.0	36.0	36.0
13	35.149693488884076	36.0	36.0	36.0	36.0	36.0
14	35.112280249261936	36.0	36.0	36.0	36.0	36.0
15	35.08172954251316	36.0	36.0	36.0	36.0	36.0
16	35.09848057074166	36.0	36.0	36.0	36.0	36.0
17	35.07575591490459	36.0	36.0	36.0	36.0	36.0
18	35.086950594369036	36.0	36.0	36.0	36.0	36.0
19	35.059118187554404	36.0	36.0	36.0	36.0	36.0
20	35.03579018151169	36.0	36.0	36.0	36.0	36.0
21	35.02374160030582	36.0	36.0	36.0	36.0	36.0
22	35.012924590435745	36.0	36.0	36.0	36.0	36.0
23	34.983342928597416	36.0	36.0	36.0	36.0	36.0
24	34.95547275482335	36.0	36.0	36.0	36.0	36.0
25	34.92625310310841	36.0	36.0	36.0	32.0	36.0
26	34.88942294849416	36.0	36.0	36.0	32.0	36.0
27	34.86648274464469	36.0	36.0	36.0	32.0	36.0
28	34.83383458992912	36.0	36.0	36.0	32.0	36.0
29	34.81243546225377	36.0	36.0	36.0	32.0	36.0
30	34.79402637239143	36.0	36.0	36.0	32.0	36.0
31	34.78464819156139	36.0	36.0	36.0	32.0	36.0
32	34.74403212954988	36.0	36.0	36.0	32.0	36.0
33	34.717405502001185	36.0	36.0	36.0	32.0	36.0
34	34.702878118652734	36.0	36.0	36.0	32.0	36.0
35	34.656140124630966	36.0	36.0	36.0	32.0	36.0
36	34.62213051708494	36.0	36.0	36.0	32.0	36.0
37	34.600111458587605	36.0	36.0	36.0	32.0	36.0
38	34.55803998691974	36.0	36.0	36.0	32.0	36.0
39	34.50160601692144	36.0	36.0	36.0	32.0	36.0
40	34.49955462622224	36.0	36.0	36.0	32.0	36.0
41	34.46752824461936	36.0	36.0	36.0	32.0	36.0
42	34.42645437336785	36.0	36.0	36.0	32.0	36.0
43	34.41064383454387	36.0	36.0	36.0	32.0	36.0
44	34.36806481178698	36.0	36.0	36.0	32.0	36.0
45	34.31267449947264	36.0	36.0	36.0	32.0	36.0
46	34.29894482799914	36.0	36.0	36.0	32.0	36.0
47	34.25513791848785	36.0	36.0	36.0	32.0	36.0
48	34.21465450140705	36.0	36.0	36.0	32.0	36.0
49	34.19373344816945	36.0	36.0	36.0	32.0	36.0
50	33.68314257948333	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	33.0
22	89.0
23	299.0
24	836.0
25	1912.0
26	3984.0
27	7380.0
28	12621.0
29	18857.0
30	26233.0
31	37456.0
32	53965.0
33	86459.0
34	186183.0
35	649287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28152114430382	18.024203875641902	11.439997936332162	26.25427704372212
2	15.698087344861033	20.671377603612516	37.924884782863025	25.705650268663426
3	18.823968312453943	24.696849668386143	28.620670596904933	27.85851142225497
4	12.068039605638525	15.901945738911923	36.57036688365821	35.459647771791346
5	13.637372709226653	37.53105411268371	34.00481759019164	14.826755587897992
6	33.79839020193404	35.87852638443923	17.013417440277376	13.30966597334935
7	29.541039328300812	30.7300537488313	21.165248870445513	18.563658052422383
8	27.573380741614123	34.13359371041953	19.587971683991874	18.705053863974467
9	27.430050524822562	13.876317813569392	18.787127914849325	39.90650374675872
10	15.444383546501719	27.247940088706297	32.70987145416611	24.59780491062587
11	37.02387148180047	20.980467112808064	22.913490634254632	19.08217077113683
12	24.553774162794017	23.761957618102347	29.853031259067524	21.83123696003611
13	30.072724425550728	20.065493434536503	25.369724715711516	24.492057424201253
14	22.806085086196177	20.369655629810108	25.779910741015378	31.04434854297834
15	24.612174778119115	28.614090760451543	22.890001427775296	23.883733033654046
16	24.629031738063105	26.084072936288983	25.654358629519947	23.632536696127964
17	23.069809000511235	26.51765605353697	26.428489183450704	23.984045762501093
18	23.929329728584523	24.961380981111915	28.314165833797745	22.79512345650582
19	24.925456312378813	25.556348764053222	26.082599103725574	23.43559581984239
20	25.021071199929995	24.838960763813724	26.66522353894833	23.474744497307952
21	25.55423012974332	24.710460987191475	25.08066930421286	24.654639578852343
22	24.80589164567223	25.809663735889206	25.77189677645184	23.612547841986725
23	23.345784148009635	25.45345682822021	26.429963016014113	24.770796007756044
24	24.153812850898806	25.588957309518655	26.483481560972916	23.773748278609624
25	24.211108091801346	25.311416214921636	26.3770892728018	24.10038642047522
26	23.055623362088422	26.114286503838873	26.96579326734862	23.864296866724086
27	24.230912716872158	25.997669502259107	26.093744962486355	23.677672818382376
28	23.308109303107482	25.694520566872846	26.420290989816735	24.57707914020293
29	23.545027887675534	25.591628631039836	26.386392840858324	24.476950640426306
30	23.597809516352633	25.623868718364413	26.904352872361493	23.873968892921457
31	24.216542849378918	25.301559959653837	26.003380603442324	24.47851658752493
32	23.49500969505483	26.0483324966263	26.056714919330698	24.399942888988168
33	23.106839043666895	25.703639905858942	26.85396622159994	24.335554828874223
34	23.733125768580653	25.929136288060572	27.164115861662392	23.173622081696383
35	24.37737482786096	25.762869552000957	26.614099971905063	23.24565564823301
36	23.2490638860359	26.068966152514033	26.667710631399082	24.014259330050987
37	24.21663496391413	25.771988890987053	25.79078025617052	24.220595888928294
38	23.582979076183328	26.15656707550168	26.087481174091863	24.17297267422313
39	24.195356506279907	25.44249519852985	26.01314474417491	24.349003551015333
40	24.836565785898184	25.409886653064422	26.457413147507612	23.296134413529785
41	23.84900585387871	25.432178370585984	27.050170181603804	23.668645593931494
42	24.48128002358132	26.34070403139263	26.422685967732278	22.755329977293766
43	23.536369121365507	25.519687179038414	26.54105314548109	24.402890554114986
44	23.101772744230175	26.052293421640467	26.553857065875707	24.292076768253644
45	23.27844842276887	26.29667328356078	26.22473183155936	24.20014646211099
46	23.266863423495124	25.386052372688383	26.561066466225252	24.78601773759124
47	24.122678137996786	25.256147493793783	26.679501291906355	23.941673076303076
48	23.77420885128569	26.483297331902488	25.57974585599735	24.162747960814475
49	23.48837744851949	25.879486553580723	26.2820270724619	24.350108925437887
50	22.88364552484559	27.15656246977492	25.573389953067643	24.386402052311844
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	45.0
2	50.0
3	179.5
4	309.0
5	321.5
6	334.0
7	304.0
8	274.0
9	362.0
10	450.0
11	664.5
12	879.0
13	1436.5
14	1994.0
15	2794.5
16	3595.0
17	4030.0
18	4465.0
19	4771.0
20	5077.0
21	5849.0
22	6621.0
23	7099.0
24	7577.0
25	8485.5
26	9394.0
27	11681.5
28	13969.0
29	16653.0
30	19337.0
31	21946.0
32	24555.0
33	29223.5
34	33892.0
35	39137.5
36	44383.0
37	48458.0
38	52533.0
39	53166.0
40	53799.0
41	56190.0
42	58581.0
43	59243.5
44	59906.0
45	64688.0
46	69470.0
47	74021.0
48	78572.0
49	78839.0
50	79106.0
51	74722.0
52	70338.0
53	68389.5
54	66441.0
55	66206.5
56	65972.0
57	63440.5
58	60909.0
59	55995.5
60	51082.0
61	45066.0
62	39050.0
63	33999.5
64	28949.0
65	25239.0
66	21529.0
67	18831.5
68	16134.0
69	14697.5
70	13261.0
71	10525.5
72	7790.0
73	6891.0
74	5992.0
75	4463.5
76	2935.0
77	2569.0
78	2203.0
79	1907.0
80	1611.0
81	1285.5
82	960.0
83	800.0
84	640.0
85	521.5
86	403.0
87	283.0
88	163.0
89	107.0
90	51.0
91	32.0
92	13.0
93	9.5
94	6.0
95	6.0
96	6.0
97	4.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014646211098880348
2	0.0042372686198018615
3	4.6057267606541976E-4
4	1.842290704261679E-4
5	0.0
6	2.763436056392518E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.211453521308395E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1085605.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.8964342147542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.69166089503899	49.326296460542814
2	13.237506684092729	16.387089232786288
3	3.6183194890484702	6.718832226655549
4	1.341097172963321	3.3203653176766816
5	0.631526887282358	1.9544631216760482
6	0.3471088314758015	1.289087937168129
7	0.21376140497061558	0.9261748118291998
8	0.14239227265095786	0.7050859149463486
9	0.09063885638366428	0.5049199810306828
>10	0.5493843729624881	6.4765194680435245
>50	0.06869305444952462	3.0298519083263367
>100	0.06311290035755747	7.365399156354531
>500	0.004347442855702184	1.7067805965484648
>1k	4.497354678312605E-4	0.2891338664154081
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.211453521308395E-5	0.0
5	0.0	0.0	0.0	9.211453521308395E-5	0.0
6	0.0	0.0	0.0	9.211453521308395E-5	9.211453521308395E-5
7	0.0	0.0	0.0	9.211453521308395E-5	9.211453521308395E-5
8	0.0	0.0	0.0	9.211453521308395E-5	9.211453521308395E-5
9	0.0	0.0	0.0	9.211453521308395E-5	9.211453521308395E-5
10	0.0	0.0	0.0	9.211453521308395E-5	9.211453521308395E-5
11	0.0	0.0	0.0	9.211453521308395E-5	9.211453521308395E-5
12	0.0	0.0	0.0	9.211453521308395E-5	3.684581408523358E-4
13	0.0	0.0	0.0	1.842290704261679E-4	6.448017464915876E-4
14	0.0	0.0	0.0	1.842290704261679E-4	6.448017464915876E-4
15	0.0	0.0	0.0	1.842290704261679E-4	7.369162817046716E-4
16	0.0	0.0	0.0	2.763436056392518E-4	8.290308169177556E-4
17	0.0	0.0	0.0	2.763436056392518E-4	8.290308169177556E-4
18	0.0	0.0	0.0	2.763436056392518E-4	0.0011053744225570073
19	0.0	9.211453521308395E-5	0.0	3.684581408523358E-4	0.0011974889577700913
20	0.0	9.211453521308395E-5	0.0	6.448017464915876E-4	0.0011974889577700913
21	9.211453521308395E-5	9.211453521308395E-5	0.0	7.369162817046716E-4	0.0014738325634093433
22	9.211453521308395E-5	9.211453521308395E-5	0.0	0.002026519774687847	0.0014738325634093433
23	9.211453521308395E-5	9.211453521308395E-5	0.0	0.004145154084588778	0.0014738325634093433
24	9.211453521308395E-5	9.211453521308395E-5	0.0	0.0077376209578990515	0.0014738325634093433
25	9.211453521308395E-5	9.211453521308395E-5	0.0	0.009764140732586898	0.0014738325634093433
26	9.211453521308395E-5	9.211453521308395E-5	0.0	0.012527576788979417	0.0016580616338355112
27	9.211453521308395E-5	9.211453521308395E-5	0.0	0.01584370005665044	0.0016580616338355112
28	9.211453521308395E-5	9.211453521308395E-5	0.0	0.02422612276104108	0.0016580616338355112
29	9.211453521308395E-5	9.211453521308395E-5	0.0	0.03767484490215133	0.0016580616338355112
30	9.211453521308395E-5	9.211453521308395E-5	0.0	0.062085196733618585	0.0016580616338355112
31	9.211453521308395E-5	9.211453521308395E-5	0.0	0.10887938062186522	0.0016580616338355112
32	9.211453521308395E-5	9.211453521308395E-5	0.0	0.16442444535535486	0.001842290704261679
33	9.211453521308395E-5	9.211453521308395E-5	0.0	0.22697021476503884	0.001842290704261679
34	9.211453521308395E-5	9.211453521308395E-5	0.0	0.2982668650199658	0.002026519774687847
35	9.211453521308395E-5	9.211453521308395E-5	0.0	0.3770247926271526	0.002026519774687847
36	9.211453521308395E-5	9.211453521308395E-5	0.0	0.48525937150252624	0.002026519774687847
37	9.211453521308395E-5	9.211453521308395E-5	0.0	0.6509734203508643	0.002026519774687847
38	9.211453521308395E-5	9.211453521308395E-5	0.0	0.8684558379889554	0.0021186343099009308
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTAAT	170	0.0	22.004906	1
CTAACGG	50	0.0025796895	22.000853	3
TTAACGG	115	8.769348E-9	21.043324	35
GTCCTAC	905	0.0	20.181295	1
TCCTACA	925	0.0	19.979153	2
TCTAGCG	255	0.0	19.842993	28
CTAGCGG	255	0.0	19.842993	29
GCAATAC	275	0.0	19.19986	37
GCCGGTC	335	0.0	19.044636	8
TTAGGAC	790	0.0	18.380459	3
CGCAATA	290	0.0	18.206764	36
CCGGTCC	340	0.0	18.117514	9
GTCCTAA	560	0.0	18.075459	1
TAGCGGC	280	0.0	18.071297	30
TAGAGTA	110	2.7960868E-6	17.999868	4
TGTAGGA	1115	0.0	17.955853	2
GTAGGAC	1105	0.0	17.919245	3
ATACGAA	295	0.0	17.898174	40
TAGACTG	160	1.1004886E-9	17.874868	5
TAGGACG	1060	0.0	17.848927	4
>>END_MODULE